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  4. Human casein alpha s1 (CSN1S1) ELISA kit

Human casein alpha s1 (CSN1S1) ELISA kit

  • 中文名称：
    人酪蛋白α-S1(CSN1S1)酶联免疫试剂盒
  • 货号：
    CSB-EL006060HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CSN1S1 ELISA Kit was designed for the quantitative measurement of Human CSN1S1 protein in breast milk. It is a Competitive ELISA kit, its detection range is 37.5 μg/mL-600 μg/mL and the sensitivity is 18.75 μg/mL.

  • 别名：
    Alpha casein ELISA Kit; alpha-S1-casein ELISA Kit; CASA ELISA Kit; CASA1_HUMAN ELISA Kit; Casein alpha s1 ELISA Kit; casein; alpha ELISA Kit; casein; alpha-S1; included ELISA Kit; Casoxin-D ELISA Kit; CSN1 ELISA Kit; CSN1S1 ELISA Kit; Csna ELISA Kit; MGC149368 ELISA Kit
  • 缩写：
    CSN1S1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    breast milk
  • 检测范围：
    37.5 μg/mL-600 μg/mL
  • 灵敏度：
    18.75 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleBreast milk(n=4)
    1:10Average %90
    Range %86-97
    1:20Average %93
    Range %89-99
    1:40Average %95
    Range %90-107
    1:80Average %92
    Range %87-103
  • 回收率：
    The recovery of human CSN1S1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Breast milk (n=5) 9587-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    6000.215 0.233 0.224
    3000.349 0.374 0.362
    1500.655 0.612 0.634
    751.358 1.326 1.342
    37.51.764 1.727 1.746
    02.631 2.419 2.525
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Important role in the capacity of milk to transport calcium phosphate.; Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
  • 基因功能参考文献：
    1. Serine-71 in alpha-S1 casein is phosphorylated in the breast milk. PMID: 28786558
    2. Results suggest that autoimmunity to insulin can occur infrequently via cross-reactivity to food protein alpha casein. PMID: 21361910
    3. Casein alpha s1 is expressed in human monocytes and stimulates expression of proinflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) via an immunomodulatory role beyond the nutritional function of CSN1S1 in milk. PMID: 21135174
    4. alphas1-Casein may be a potential biomarker for early identification of BPH patients. PMID: 16683014
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-casein family
  • 组织特异性：
    Mammary gland specific. Secreted in milk.
  • 数据库链接：

    HGNC: 2445

    OMIM: 115450

    KEGG: hsa:1446

    STRING: 9606.ENSP00000246891

    UniGene: Hs.3155