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Human apelin 36(AP36)ELISA Kit

  • 中文名称:
    人apelin 36(AP36)酶联免疫试剂盒
  • 货号:
    CSB-E13566h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human AP36 ELISA Kit was designed for the quantitative measurement of Human AP36 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 22.86 pg/mL-400 pg/mL and the sensitivity is 14.29 pg/mL.

  • 缩写:
    AP36
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    22.86 pg/mL-400 pg/mL
  • 灵敏度:
    14.29 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human AP36 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %102
    Range %89-106
    1:2Average %95
    Range %92-99
    1:4Average %88
    Range %85-92
    1:8Average %99
    Range %95-103
  • 回收率:
    The recovery of human AP36 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104100-108
    EDTA plasma (n=4)9391-97
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.128 1.958 2.043 2.007
    228.51.114 1.070 1.092 1.056
    91.40.336 0.344 0.340 0.304
    45.70.144 0.150 0.147 0.111
    22.860.082 0.084 0.083 0.047
    00.035 0.036 0.036
  • 数据处理:
  • 货期:
    3-5 working days

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产品评价

问答及客户评论

 客户评论
  • Sample type: Serum
    Sample species: Human
    Review: The use of the kit was very fast and easy and very excellent values were obtained.