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  4. Human anti glial fibrillary acidic protein (GFAP) autoantibody ELISA kit

Human anti glial fibrillary acidic protein (GFAP) autoantibody ELISA kit

  • 中文名称：
    人抗神经胶质纤维酸性蛋白(GFAP)自身抗体酶联免疫试剂盒
  • 货号：
    CSB-EQ027671HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 缩写：
    GFAP auto-Ab
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    Request Information
  • 灵敏度：
    Request Information
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    qualitative
  • 测定方法：
    Indirect
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 标准曲线：
    Test parameter specification test result
    Positive control >0.8 1.750
    Negative control <0.2 0.148
    Positive rate 10，Positive 100%
    Negative rate 10，Negative 100%
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
The customer also needs to know which isotype can be detected by the kit?
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry!
CSB-EQ027671HU
This kit is for qualitative determination of GFAP autoantibody in human serum and plasma.
There is no capture antibody and detection antibody. There is no standard either in it.
The plate is coated with specific antigen.
Q:

If there is no detection antibody in the kit please can you clarify how the signal is created in the color reaction. Is there labeled antigen rather than a detection antibody?
The principle seems to mention the incorporation of anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). If this is correct then which types of Ig are detected, is it all of them or just certain such as IgG and IgM.

A:
CSB-EQ027671HU
This is an indirect kit. There is no detection antibody in the kit. The target is GFAP autoantibody（it is total, including IgG/IgM）. The plate is coated with specific antigen( the antigen binds to the target specifically). The secondary antibody in the kit is anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP)（the secondary antibody binds to the target). Then add TMB substrate, and the color will be developed under the catalysis of HRP.
Q:

The customer specifically asked what isotypes this kit would detect. The answer to their question lies in the secondary antibody being used in the assay. Most commercial antibodies against Immunoglobulins will be specific to a certain isotype IgG, IgM, IgA etc.
I have tried to find examples of commercially available antibodies which would recognize all immunoglobulins however so far I have not been able to find any. Is the secondary antibody you use a mixture of antibodies that will bind to several different isotypes? If so do you know which ones?

A:
The secondary antibody is anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP).
It is mixed. It detects total GFAP autoantibody（including IgG/IgM）.
Q:

Regarding your ELISA kit CSB-EQ027671HU, could you please advise what isotypes this kit is capable of detecting? All isotypes, or specific ones?

A:
Thanks for your inquiry!
CSB-EQ027671HU
This kit detects total antibodies, including all isotypes.