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  4. Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit

Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit

  • 中文名称：
    人抗双链DNA(dsDNA)抗体/天然DNA抗体(IgG)酶联免疫试剂盒
  • 货号：
    CSB-E04911h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The product CSB-E04911h is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of human anti-double-stranded DNA (dsDNA) antibody (IgG) (dsDNA Ab (IgG)) in serum. Based on the Indirect-ELISA technique in combination with enzyme-substrate chromogenic reaction, the dsDNA Ab (IgG) levels can be calculated referring to the standard curve. This Human dsDNA Ab (IgG) ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 15%, good linearity, and lot-to-lot consistency.

    This assay employs the qualitative enzyme immunoassay technique. Samples are pipetted into the wells with anti-human IgG conjugated HRP. Any antibodies specific for the antigen present will bind to the pre-coated antigen-specific for dsDNA Ab (IgG) on the microwell plate. Following a wash to remove any unbound reagent, substrate A and B solutions are added to the wells and color develops in proportion to the amount of human dsDNA Ab (IgG) bound in the initial step. The color development is terminated and the intensity of the color is measured.

    Anti-dsDNA antibody (IgG) is an isoform of anti-dsDNA antibodies that are related to active systemic lupus erythematosus (SLE) and lupus nephritis (LN). Anti-dsDNA antibodies recognize diverse DNA structures, including single-stranded (ss)DNA, Z-DNA, bent or elongated dsDNA, DNA: RNA hybrids, locked-nucleic acids, and peptide: nucleic acid hybrids. Xiaoyu Wang and Yumin Xia demonstrated that anti-dsDNA IgG contributes to renal fibrosis by downregulating suppressor of cytokine signaling 1 (SOCS1) and activating Jak-STAT1 signals, which modulate the expression of profibrotic genes, TGF-β1, platelet-derived growth factor B, and connective tissue growth factor.

  • 别名：
    N/A
  • 缩写：
    dsDNA Ab (IgG)
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Autoimmunity
  • 测定原理：
    qualitative
  • 测定方法：
    Indirect
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 标准曲线：
    Test parameter specification test result  
    Positive control >0.6 1.246  
    Negative control <0.15 0.053  
    Positive rate 10,Positive 100%  
    Negative rate 10,Negative 100%  
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with antigen-specific for dsDNA Ab (IgG). This dismountable microplate can be divided into 12 x 8 strip plates.
    • Negative Control (1 x 800 μl)
    • Positive Control (1 x 800 μl)
    • HRP-conjugate (1 x10 ml) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Sample Diluent (1 x 20 ml) --- Dilute the standard and sample solution.
    • Wash Buffer (25 x concentrate) (1 x20 ml) --- Wash away unbound or free substances.
    • Substrate A 1 x 5 ml --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Substrate B 1 x 5 ml --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Stop Solution (1 x 5 ml) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 货期：
    3-5 working days

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