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  4. Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit

Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit

  • 中文名称：
    人抗环胍氨酸肽(CCP)抗体酶联免疫试剂盒
  • 货号：
    CSB-E09077h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody, ACCPA) ELISA Kit is designed to qualitatively detect the anti-CCP antibody in the serum. It employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CCP antigen. Samples are pipetted into the wells with anti-human IgG conjugated HRP. Any antibodies specific for CCP present in the samples will bind to the pre-coated CCP antigen. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human anti-CCP antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm.

    This assay has high sensitivity and excellent specificity for detection of human anti-CCP antibody. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

    ACCPA is a type of autoantibodies against citrullinated peptides and proteins. They target healthy tissues in the joints. And they are detected in the majority of patients suffering from rheumatoid arthritis (RA). An ACCPA test is helpful for the diagnosis of RA, along with or after a rheumatoid factor (RF) test. ACCPA is predictive of the progression of patients, indicating more erosive disease or increase joint involvement.

  • 缩写：
    ACCPA
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum
  • 检测范围：
    Request Information
  • 灵敏度：
    Request Information
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Autoimmunity
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 标准曲线：
                 
                 
    Test parameter specification test result  
    Positive control >1 2.078  
    Negative control <0.1 0.075  
    Positive rate 10,Positive 100%  
    Negative rate 10,Negative 100%  
  • 本试剂盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with CCP antigen.
    • Negative Control (1 x 0.8 ml)
    • Positive Control (1 x 0.8 ml)
    • HRP-conjugated IgG antibody(1 x 10m)l --Bind to the human anti-CCP antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Sample Diluent (2 x 20 ml) --Dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
    • Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
    • Stop Solution (1 x 5ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I would like to order Human ELISA kit for anti-CCP3.

A:
Thanks for your inquiry.
Regarding your request,we have this kit for you:#CSB-E09077h,Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit. I will ask our distributor in your area to send you a quote by email.
Q:

I found out that that it was a qualitative kit. Is there any way for me to obtain the concentration of the positive control?
I can only get the volume from the kit and not the concentration. Would like to have the concentration so that i can use it for quantitative analysis later on.

A:
Thanks for your inquiry.
Yes this kit is for qualitative determination.Thus you can not obtain the concentration.It can not be used for quantitative analysis.