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  4. Human alpha-L-fucosidase,AFU ELISA Kit

Human alpha-L-fucosidase,AFU ELISA Kit

  • 中文名称：
    人αL岩藻糖苷酶(AFU)酶联免疫试剂盒
  • 货号：
    CSB-E08348h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FUCA2 ELISA Kit was designed for the quantitative measurement of Human FUCA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 μU/mL-2000 μU/mL and the sensitivity is 7.8 μU/mL.

  • 别名：
    Alpha L fucosidase 2 ELISA Kit; Alpha L fucosidase fucohydrolase 2 ELISA Kit; Alpha-L-fucosidase 2 ELISA Kit; Alpha-L-fucoside fucohydrolase 2 ELISA Kit; dJ20N2.5 ELISA Kit; FUCA 2 ELISA Kit; FUCA2 ELISA Kit; FUCO2 ELISA Kit; FUCO2_HUMAN ELISA Kit; Fucosidase alpha L 2 plasma ELISA Kit; Fucosidase; alpha-L; plasma ELISA Kit; MGC1314 ELISA Kit; Plasma alpha L fucosidase ELISA Kit; Plasma alpha-L-fucosidase ELISA Kit; RP1 20N2.5 ELISA Kit
  • 缩写：
    FUCA2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 μU/mL-2000 μU/mL
  • 灵敏度：
    7.8 μU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AFU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %88
    Range %81-95
    1:40Average %91
    Range %86-97
    1:80Average %97
    Range %91-102
    1:160Average %102
    Range %97-107
  • 回收率：
    The recovery of human AFU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10395-110
    EDTA plasma (n=4)9385-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µU/mlOD1OD2AverageCorrected
    20002.720 2.546 2.633 2.546
    10002.235 2.200 2.218 2.131
    5001.554 1.633 1.594 1.507
    2500.821 0.845 0.833 0.746
    1250.471 0.445 0.458 0.371
    62.50.311 0.302 0.307 0.220
    31.250.185 0.188 0.187 0.100
    00.084 0.090 0.087 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
  • 基因功能参考文献：
    1. Genetic polymorphisms within the FUCA2 and IL18 gene regions are also associated with diastolic function in SCD, likely by affecting expression levels of the genes PMID: 27636371
    2. serum alpha-L-fucosidase was identified as a useful marker for close monitoring of patients during post-treatment follow-up PMID: 18521898
    3. Findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for diagnosis and therapy of H. pylori-related diseases. PMID: 19666478
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycosyl hydrolase 29 family
  • 数据库链接：

    HGNC: 4008

    OMIM: 136820

    KEGG: hsa:2519

    STRING: 9606.ENSP00000002165

    UniGene: Hs.17680