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  4. Human Zyxin(ZYX) ELISA kit

Human Zyxin(ZYX) ELISA kit

  • 中文名称：
    人斑联蛋白(ZYX)酶联免疫试剂盒
  • 货号：
    CSB-EL027165HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ZYX ELISA Kit was designed for the quantitative measurement of Human ZYX protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    ZYX ELISA Kit; Zyxin ELISA Kit; Zyxin-2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human ZYX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %84-94
    1:2Average %94
    Range %90-98
    1:4Average %92
    Range %85-96
    1:8Average %91
    Range %88-95
  • 回收率：
    The recovery of Human ZYX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
    EDTA plasma (n=4)8884-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.813 2.618 2.716 2.611
    7502.318 2.414 2.366 2.261
    3751.825 1.722 1.774 1.669
    187.50.984 0.968 0.976 0.871
    940.533 0.567 0.550 0.445
    470.301 0.322 0.312 0.207
    23.50.192 0.185 0.189 0.084
    00.104 0.105 0.105
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
  • 基因功能参考文献：
    1. Study findings identify zyxin as a physiological regulator of endothelial exocytosis through reorganizing local actin network in the final stage of exocytosis. Zyxin downregulation inhibits the secretion of VWF from human primary endothelial cells. PMID: 28256511
    2. these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation. PMID: 29967145
    3. Increased zyxin expression is associated with asthma. PMID: 28278518
    4. Zyxin binding to Ptc2 is due to the interaction of Zyxin 2nd LIM-domain (530-590 aa) with the under-membrane region of the cytoplasmic C-terminus of Ptc2 (1159-1412 aa). PMID: 27125030
    5. ZYX gene may be aging-related gene and involved in Osteoporosis. PMID: 25771989
    6. key regulator of stretch-induced gene expression at the cellular level PMID: 26071033
    7. Matrix density alters zyxin phosphorylation, which limits peripheral process formation and extension in endothelial cells invading 3D collagen matrices. PMID: 25038525
    8. Zyxin, which is thought to be essential for tight cell-to-cell junctions, decreased 1.8-fold in TNF-alpha-treated human brain microvascular endothelial cells. PMID: 25025691
    9. Integrin-beta5 and zyxin mediate formation of ventral stress fibers in response to transforming growth factor beta. PMID: 24036928
    10. Zyxin is a novel target for beta-amyloid peptide activities in Alzheimer's disease. PMID: 23330981
    11. Zyxin is suggested to promote growth, migration and invasiveness of fibroblastic type oral squamous cell carcinoma cells by upregulating Rac1 and Cdc42. PMID: 23292068
    12. A stretch-induced signaling pathway in vascular cells leads to the activation of zyxin, a cytoskeletal protein specifically involved in transducing mechanical stimuli. PMID: 23233529
    13. RA-induced cancer cell cytotoxicity was significantly impaired by Zyxin or PTOV1. Overall, our findings suggest that Zyxin and PTOV1 should be considered as critical determinants in cancer therapy with retinoids PMID: 23321499
    14. Zyxin is a transforming growth factor-beta (TGF-beta)/Smad3 target gene that regulates lung cancer cell motility via integrin alpha5beta1. PMID: 22778267
    15. Zyxin phenotype is mediated by partners alpha-actinin and p130Cas and ensures that motile cells in a three-dimensional matrix explore the largest space possible in minimum time. PMID: 22395610
    16. Depletion of nectin-2 from L cells expressing E-cadherin results in a loss of zyxin localization to cell-cell contacts, demonstrating that the zyxin-nectin interaction plays a critical role in zyxin targeting to these sites. PMID: 22056563
    17. LIM domain of zyxin recruits zyxin to force-bearing sites at the leading edge of migrating cells PMID: 21889443
    18. Findings suggest an unforeseen role for Zyxin in DNA damage-induced cell fate control through modulating the HIPK2-p53 signaling axis. PMID: 21248071
    19. Zyxin, whose expression is negatively regulated by microRNA-16 (miR-16), is confirmed to be a direct target gene of miR-16. Overexpression of zyxin could also restrain cell movement and enhance cell-cell adhesion. PMID: 21360639
    20. Stretch appears to be an important factor in the development of endothelial dysfunction with zyxin as a potential therapeutic target to interfere with these early changes in endothelial cell phenotype PMID: 20705921
    21. The LIM domain protein zyxin was identified as an interaction partner for human synemin. PMID: 19853601
    22. This study establishes a crucial role of zyxin in thrombin signaling in endothelial cells and provides evidence for a novel PAR-1 signaling pathway mediated by zyxin. PMID: 19690217
    23. LIM-nebulette, Lasp-1, and zyxin may play an important role in the organization of focal adhesions PMID: 15004028
    24. Within Ewing tumor cells, zyxin is expressed at very low levels and remains diffusely distributed throughout the cytoplasm, instead of concentrating in actin-rich dynamic structures. Zyxin has tumor suppressor activity in Ewing tumor cells. PMID: 15748890
    25. zyxin indirectly enables the interaction of ZNF384/CIZ/NMP4 with p130CAS PMID: 16510139
    26. A sarch for genes upregulated by thymosin beta4 identified zyxin in SiHA cells in the presence of exogenously added thymosin beta4 and when thymosin beta4 is overexpressed using adenoviral vectors. PMID: 16956606
    27. These results suggest that zyxin is involved in mechanical-force-dependent facilitation of actin polymerization at focal adhesions. PMID: 18682496
    28. We identified zyxin as an interactor with endoglin in human umbilical vein vascular endothelial cells and this interaction is localized within the 47-amino acid carboxyl-terminal cytosolic domain of endoglin, and maps within zyxin residues 326-572. PMID: 15084601

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Note=Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.
  • 蛋白家族：
    Zyxin/ajuba family
  • 数据库链接：

    HGNC: 13200

    OMIM: 602002

    KEGG: hsa:7791

    STRING: 9606.ENSP00000324422

    UniGene: Hs.490415