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  4. Human Vitamin D-binding protein,DBP ELISA Kit

Human Vitamin D-binding protein,DBP ELISA Kit

  • 中文名称：
    人维生素D结合蛋白(DBP)酶联免疫试剂盒
  • 货号：
    CSB-E11859h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        GC (guanylate cyclase)，是一种将GTP转化为环GMP的酶，可以通过内源性鸟苷酸肌醇二磷酸（cGMP）的信号通路影响多个细胞和生物学过程，包括细胞增殖、分化、凋亡等。生物学含义：GC在心血管系统中发挥重要作用，参与调节血管张力、心率和血压，对于一些心血管疾病的发生和发展具有重要意义。
        j9九游会登录入口首页生物所提供的Human Vitamin D-binding protein,DBP ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测人血清、血浆、组织匀浆样本中的GC，其灵敏度为0.078 ng/ml，检测范围为0.3125 ng/ml - 20ng/ml。
     
  • 别名：
    DBP ELISA Kit; DBP/GC ELISA Kit; GC ELISA Kit; Gc globulin ELISA Kit; Gc-globulin ELISA Kit; GRD3 ELISA Kit; Group specific component ELISA Kit; Group specific component vitamin D binding protein ELISA Kit; Group-specific component ELISA Kit; hDBP ELISA Kit; VDB ELISA Kit; VDBG ELISA Kit; VDBP ELISA Kit; Vitamin D binding alpha globulin ELISA Kit; Vitamin D-binding protein ELISA Kit; VTDB_HUMAN ELISA Kit
  • 缩写：
    GC
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.3125 ng/ml - 20ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 本试剂盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with human DBP.
      • Standard(Freeze-dried) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated DBP antibody(100 x concentrate) (1 x 60 μl) --Bind to the DBP, and HRP catalyzes the TMB to elicit a chromogenic reaction.
      • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated DBP antibody solution.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    GC是一种被肾上腺分泌的类固醇激素，已被广泛研究与炎症反应、免疫调节、糖代谢等方面的关系。近期研究发现，GC还与肝脏脂质代谢和肝纤维化的发病机制密切相关。研究结果表明，GC通过调节肝脂代谢和减少炎症反应，能够改善脂肪肝和肝纤维化等疾病的病理表现，为相关疾病的治疗提供新的思路。
  • 功能：
    Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
  • 基因功能参考文献：
    1. VDBP in the cervicovaginal fluid (CVF) independently predicts intra-amniotic infection and imminent preterm delivery in women with PTL, whereas in women with preterm premature rupture of membranes, an elevated VDBP level in CVF is not associated with increased risks of these two outcome variables. PMID: 29879190
    2. SNPs of the VDR and GC genes are associated with vitamin D deficiency in postmenopausal Mexican women. PMID: 30150596
    3. GC rs7041 genotype modified the effects of pregnancy on maternal and placental vitamin D metabolism. PMID: 29196501
    4. Urinary VDBP correlated with proteinuria and renal SLE disease activity index, and predicted the development of proteinuria in lupus nephritis. PMID: 29958502
    5. Findings implied that VDBP rs7041-G and rs3733359-T variants may contribute to increased susceptibility to HCV infection in a high-risk Chinese population. PMID: 30218750
    6. Polymorphisms of VDBP rs4588 and rs2282679 may play a potentially important role in epilepsy susceptibility. PMID: 29993274
    7. The study findings suggested a possible clinical application of uVDPB as an early and a good marker for the detection of early renal disease in type 2 diabetes mellitus Saudi patients. PMID: 29850609
    8. GC gene variant has no effect on 25-hydroxyvitamin D levels. PMID: 28892641
    9. genetic association study in population in north India: Data suggest (1) GT allele of VDBP SNP rs7041, (2) VDBP allelic combination (GC1F/1F: T allele rs4588; C allele rs7041), and (3) GA allele of CYP2R1 SNP rs2060793 are associated with vitamin D deficiency in women with PCOS (polycystic ovarian syndrome) in population studied. (VDBP = vitamin D-binding protein; CYP2R1 = cytochrome P450 family 2 subfamily R member 1) PMID: 28008453
    10. The A allele of VDBP gene polymorphism might be a potential risk factor for progression of chronic urticarial. PMID: 29165650
    11. The present study indicates an association between VDR and vitamin D binding protein Single Nucleotide Polymorphisms and Type 1 Diabetes Mellitus among Turkish subjects. PMID: 29506625
    12. Survival analyses showed that the vitamin D binding protein C allele was correlated with poor disease-free survival (DFS). PMID: 29409465
    13. These findings showed that racial/ethnic variations in bioavailable vitamin D do not explain the lack of association between 25-hydroxyvitamin D and multiple sclerosis in blacks and Hispanics; and they further challenge the biological plausibility of vitamin D deficiency as causal for MS. PMID: 29414925
    14. We observed associations between VDR, GC, and CYP27B1 variants and maternal 25-hydroxyvitamin D concentration. Our results provide additional support for a possible role of genetic variation in vitamin D metabolism genes on vitamin D status during pregnancy. PMID: 29175129
    15. Data show that vitamin D-binding protein (DBP) is elevated in the CSF of temporal lobe epilepsy patients. PMID: 19109932
    16. In a Turkish Parkinson disease cohort, rs7041 of GC was associated with the PD risk. The homozygous major allele carriers for rs2282679, rs3755967 and rs2298850 of GC gene in PD patients with slower progression had significantly higher levels of serum 25OHD. This is the first study demonstrating GC gene as a risk factor for PD. PMID: 27282160
    17. Vitamin D levels are associated with severity of liver fibrosis in chronic hepatitis C genotype 1 patients. Although the rs7041 and rs4588 GC polymorphisms are strong predictors of vitamin D levels, they do not play a direct role in liver fibrosis. PMID: 28809744
    18. Vitamin D binding protein polymorphisms were frequently associated to fibrosis grade in chronic hepatitis C suggesting that they could be used as disease evaluation markers to understand the mechanisms underlying the virus-host interaction. PMID: 29465575
    19. Findings indicate that Gc globulin (GC) rs16847024, retinoid X receptor gamma (RXRG) rs17429130 and retinoid X receptor alpha (RXRA) rs4917356 were candidate susceptibility markers for gestational diabetes mellitus (GDM) in Chinese females. PMID: 27636996
    20. Increased circulating levels of vitamin D binding protein in multiple sclerosis patients. PMID: 25590278
    21. Results show that APOE, DBP and AGT identified were associated with survival outcomes in metastatic colorectal cancer patients treated with chemotherapy and bevacizumab PMID: 25428203
    22. results provide a direct evidence of cross-talk among the structural domains of DBP PMID: 18035050
    23. DBP strictly inhibited the production of 1alpha,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3-induced T cell responses. PMID: 25230725
    24. 25(OH)D2 half-life was shorter than 25(OH)D3 half-life, and half-lives were affected by DBP concentration and genotype PMID: 24885631
    25. The interaction between vitamin D status, as measured by circulating 25(OH)D and DBP rs2282679 genotypes, modified the association between 25(OH)D and BMD and bone markers PMID: 25890042
    26. Data suggest that, while low circulating levels of DBP contribute to low circulating levels of 25-hydroxyvitamin D in patients with PHPT (primary hyperparathyroidism), low DBP alone is not responsible for the hypovitaminosis D observed in these patients; vitamin D metabolism is likely to be generally disturbed in PHPT. [EDITORIAL] PMID: 27858283
    27. the purpose of this investigation was to assess the relative degree of O-linked trisaccharide glycosylation of DBP in breast, colorectal, pancreatic, and prostate cancer patients compared with healthy individuals. PMID: 19642159
    28. Comparison of Two ELISA Methods and Mass Spectrometry for Measurement of Vitamin D-Binding Protein: Implications for the Assessment of Bioavailable Vitamin D Concentrations Across Genotypes. PMID: 27250744
    29. Single-nucleotide polymorphisms in the vitamin D binding protein genewere independently associated with lower 25-hydroxy-vitamin Dand higher 24,25-dihydroxy-vitamin D. PMID: 27313313
    30. genetic association studies in a population in Brazil: Data suggest that SNPs in RXRG (rs2134095) and GC (rs7041) are associated with low-density lipoprotein cholesterol levels and hypercholesterolemia in the population studied; there was no apparent association with an SNP in VDR (rs2228570). (RXRG = retinoid X receptor gamma; GC = vitamin D-binding protein; VDR = vitamin D receptor) PMID: 27721113
    31. The study strongly suggests that there might have an association of vitamin D, and vitamin D-binding protein gene (codon 416 & 420) polymorphisms with the occurrence of type 2 diabetes mellitus PMID: 28888576
    32. Low serum vitamin D-binding protein concentrations are associated with type 1 diabetes. PMID: 27103201
    33. rs7041 polymorphism of Vitamin D Binding Protein does not affect platelet reactivity or the rate of high-residual platelet reactivity among patients receiving dual antiplatelet therapy with clopidogrel or ticagrelor. PMID: 28433569
    34. High VDBG concentration was associated with coronary heart disease events in all racial and ethnic groups. PMID: 28472285
    35. SNPs rs7041 and rs4588 of VDBP are not associated with the levels of 25-hydroxyvitamin D nor with the prevalence and extent of CAD. 25-hydroxyvitamin D levels but not VDBP genetic status independently predicted the occurrence of coronary lesions at angiography. PMID: 28779988
    36. Genetic variant in vitamin D-binding protein is associated with metabolic syndrome. PMID: 28278285
    37. Study suggests higher (versus lower) circulating DBP may be independently associated with a decreased prostate cancer risk in black men independent of 25(OH)D status. PMID: 28369777
    38. The data demonstrate a relationship between the diurnal rhythms of 1,25(OH)2D and DBP, possibly to maintain free 1,25(OH)2D concentrations. PMID: 28732681
    39. the interaction between 25(OH)D status and some maternal GC variants influence the birth weight of infants PMID: 28241988
    40. The results of this study suggest that DBP is not involved in the pathogenesis of MS in Italians. PMID: 28284354
    41. This report summerizes current understanding of vitamin D-binding protein, its genetic determinants, and their effect on calcifediol concentrations. (Review) PMID: 27742848
    42. Allelic variations in CYP2R1 and GC affect vitamin D levels, but variant alleles on VDR and DHCR7 were not correlated with vitamin D deficiency. PMID: 26038244
    43. Patients with primary hyperparathyroidism have lower serum VDBP than controls. PMID: 27682354
    44. VDBP polymorphism is associated with vitamin D deficiency. PMID: 27768857
    45. genome-wide association study in population of children/adolescents in Colorado: Data suggest that VDBP is an autoantigen in development of autoimmune type 1 diabetes (T1D) in the population studied; serum 25-hydroxyvitamin D levels are negatively correlated with VDBP-autoantibody levels in patients in whom T1D developed during the winter. [META-ANALYSIS] PMID: 26983959
    46. VDBP rs222020 C > T polymorphisms may be predisposition factors of adolescent idiopathic scoliosis and the efficacy of brace treatment. PMID: 27856225
    47. findings do not support a role of an independent effect of the investigated vitamin D-related gene variants, VDBP and CYP27B1, in the risk of Multiple Sclerosis PMID: 27904983
    48. The polymorphisms in the VDR and VDBP genes appeared to be responsible for host susceptibility to human tuberculosis in a Taiwanese population. PMID: 26869016
    49. No association was observed between GC or VDR polymorphisms and breast cancer risk. associations between vitamin D-related genetic variants and breast cancer were not observed overall, although the relationships between vitamin D pathway polymorphisms and breast cancer may be modified by menopausal status and breast tumour subtype PMID: 26631034
    50. In a Pakistani population, no statistically significant associations between SNPs in VDR, DBP, and CYP2R1 and tuberculosis was demonstrated. PMID: 27160686

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.
  • 数据库链接：

    HGNC: 4187

    OMIM: 139200

    KEGG: hsa:2638

    STRING: 9606.ENSP00000421725

    UniGene: Hs.418497