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  4. Human Vitamin B12,VB12 ELISA Kit

Human Vitamin B12,VB12 ELISA Kit

  • 中文名称：
    人维生素B12(VB12)酶联免疫试剂盒
  • 货号：
    CSB-E07903h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        VB12指的是维生素B12（Vitamin B12），是人体必需的维生素之一。VB12是一种脂溶性维生素，可以促进人体红细胞的生成，维持神经系统的正常运作，并参与细胞分裂。VB12大多数存在于动物性食品中，如肉类、奶类、蛋类和鱼类等。人体摄入VB12后，需要胃酸和一种叫因子的蛋白质的辅助才能被吸收利用。
        j9九游会登录入口首页生物所提供的Human Vitamin B12,VB12 ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测人血清、血浆样本中的VB12，其灵敏度为1.498 pg/mL，检测范围为1.56 pg/mL-100 pg/mL。
     
  • 缩写：
    VB12
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    1.498 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 线性度：

     

    To assess the linearity of the assay, samples were spiked with high concentrations of human VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

  • 标准曲线：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本试剂盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody.
      • Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl)
      • HRP-avidin (100 x concentrate) (1 x 120 μl)
      • Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12
      • HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Protocol P9, step 3 - Set a Blank well without any solution.
This is a completely empty well with no solution or liquid at all? Am I correct?
May I know if this is S0. If it is S0, do we add the wash buffer, TMB and stop solution?

A:
Thanks for your inquiry.
Actually step3 is just only one procedure during the whole experiment. You need to check the full steps according our manual.
This kit is competitive one and the blank is different from S0 indeed. The blank should be added Wash Buffer+TMB Substrate+Stop Solution.
You could see it from the protocol. Pls read the manual carefully.

靶点详情

  • 最新研究进展：
    维生素B12是一种必需营养素，可通过摄入动物性食品或补充剂获得。近年来的研究表明，维生素B12缺乏与多种慢性疾病如糖尿病、心血管疾病和某些癌症的发病率增加有关。