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  4. Human Vascuar endothelial cell growth factor receptor 3,VEGFR-3/Flt-4 ELISA Kit

Human Vascuar endothelial cell growth factor receptor 3,VEGFR-3/Flt-4 ELISA Kit

  • 中文名称：
    人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)酶联免疫试剂盒
  • 货号：
    CSB-E04765h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        VEGFR-3/Flt-4是一种血管内皮生长因子受体家族成员，参与血管生成和淋巴管生成等过程。VEGFR-3/Flt-4的功能异常与许多疾病有关，如心血管疾病、肿瘤和淋巴水肿等。
        j9九游会登录入口首页生物所提供的Human Vascuar endothelial cell growth factor receptor 3,VEGFR-3/Flt-4 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的VEGFR-3/Flt-4，其灵敏度为0.039 ng/mL，检测范围为0.156 ng/mL-10 ng/mL。
     
  • 别名：
    EC 2.7.10.1 ELISA Kit; flt 4 ELISA Kit; FLT-4 ELISA Kit; FLT4 ELISA Kit; FLT41 ELISA Kit; Fms related tyrosine kinase 4 ELISA Kit; Fms-like tyrosine kinase 4 ELISA Kit; LMPH1A ELISA Kit; PCL ELISA Kit; Soluble VEGFR3 variant 1 ELISA Kit; Soluble VEGFR3 variant 2 ELISA Kit; Soluble VEGFR3 variant 3 ELISA Kit; Tyrosine protein kinase receptor FLT4 ELISA Kit; Tyrosine-protein kinase receptor FLT4 ELISA Kit; Vascular endothelial growth factor receptor 3 ELISA Kit; Vascular endothelial growth factor receptor 3 precursor ELISA Kit; VEGF R3 ELISA Kit; VEGFR 3 ELISA Kit; VEGFR-3 ELISA Kit; VEGFR3 ELISA Kit; VGFR3_HUMAN ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-3/Flt-44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 98    
    Range % 91-105    
    1:2 Average % 90    
    Range % 85-96    
    1:4 Average % 95    
    Range % 85-105    
    1:8 Average % 92    
    Range % 83-98    
  • 回收率：
    The recovery of human VEGFR-3/Flt-44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 86-100    
    EDTA plasma (n=4) 93 87-98    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.549 2.516 2.533 2.420    
    5 1.517 1.557 1.537 1.424    
    2.5 0.796 0.758 0.777 0.664    
    1.25 0.472 0.489 0.481 0.368    
    0.625 0.302 0.315 0.309 0.196    
    0.312 0.259 0.246 0.253 0.140    
    0.156 0.164 0.171 0.168 0.055    
    0 0.111 0.115 0.113 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    VEGFR-3/Flt-4，是一种受体酪氨酸激酶，主要在淋巴管内皮细胞和淋巴组织中表达，并在淋巴管生成和淋巴细胞迁移中发挥重要作用。最新的研究表明，VEGFR-3/Flt-4在恶性肿瘤的发展中发挥了重要作用，它通过调节肿瘤血管生成和淋巴管生成来促进肿瘤的生长和转移。
  • 功能：
    Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
  • 基因功能参考文献：
    1. VEGFR3 has a role in lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms PMID: 29615616
    2. These results suggest functional interactions among ATX, VEGFR-2, and VEGFR-3 in the modulation of hemovascular and lymphovascular cell activation during vascular development. PMID: 30456868
    3. VEGFR-3 and CAV3 expression demonstrated immunohistochemically in SMCs of the tunica media of SV grafts predicted their early restenosis in triple-vessel CAD patients. CAV2 protein expression in SMCs of ITA grafts indicated the risk of early graft failure both in double-vessel and triple-vessel CAD subjects. PMID: 29557990
    4. Single nucleotide polymorphism of VEGFR3 is associated with relapse in gastroenteropancreatic neuroendocrine neoplasms. PMID: 29787601
    5. VEGFR3 single nucleotide polymorphisms association with lymphedema caused by Wuchereria bancrofti. PMID: 29122006
    6. The results imply a very good sensitivity of VEGFR-3 in ESCC. VEGFR-3 may be a good diagnostic biomarker for ESCC. PMID: 28447586
    7. VEGFR-3 expression was associated with depth of invasion and lymph node metastasis in gastric cancer PMID: 28939099
    8. The finding of rare LAMA5 variants together with FLT4 in Milroy disease suggests that these mutations may be co-responsible for these disorders and most likely interfere with the function of lymphatics. PMID: 29908552
    9. Rare inherited and de novo variants in 2,871 congenital heart disease probands identified GDF1, MYH6, and FLT4 as causative genes. PMID: 28991257
    10. There was a significant decrease in VEGFR3 expression in pulmonary arterial endothelial cells from pulmonary arterial hypertension patients. PMID: 28356442
    11. By treating LECs with VEGF-C156S and analyzing subsequent changes in gene expression, we identified several 'immediate early' transcription factors that showed a rapid transient upregulation VEGFR-3 stimulation. these results reveal an important and unanticipated role of HOXD10 in the regulation of VEGFR-3 signaling in lymphatic endothelial cells, and in the control of lymphangiogenesis and permeability. PMID: 27199372
    12. These results indicate that VEGF-C-induced MSC osteogenesis is mediated through VEGFR2 and VEGFR3, and followed the activation of the ERK/RUNX2 signaling pathway. PMID: 28163024
    13. Assessment of VEGFR-2/VEGFR-3 on tumor samples might serve as a putative prognostic factor in renal cell carcinoma cases, identifying a subset of patients that may benefit from antiangiogenic treatments targeting VEGFR receptors. PMID: 27837630
    14. This study suggests that NRP1 expression and LVD are independent factors that are likely to predict the risk of LN metastasis in squamous cell carcinoma (SCC)of the tongue, whereas the expression of VEGFC, VEGFR3, CCR7, and SEMA3E are nonindependent predictive factors PMID: 27666723
    15. The summarizes the structure and function features of pathway-related molecules of VEGFC/D-VEGFR3/NRP2 axis, stages of various tumors and their molecular mechanisms and significances in tuthe expression changes of these molecules in different anatomic organs or histopathologic types or development lymphatic metastasis. PMID: 27527412
    16. this study uncovers a unique molecular mechanism of lymphangiogenesis in which galectin-8-dependent crosstalk among VEGF-C, podoplanin and integrin pathways plays a key role. PMID: 27066737
    17. Report FLT4 genetic alterations in angiosarcomas. PMID: 26735859
    18. Data indicate that foretinib suppresses angiogenesis and lymphangiogenesis by blocking vascular endothelial growth factor receptors PMID: 25909285
    19. Genistein suppresses FLT4 and inhibits human colorectal cancer metastasis. PMID: 25605009
    20. A Novel Missense Mutation in FLT4 Causes Autosomal Recessive Hereditary Lymphedema PMID: 26091405
    21. Missense mutations in VEGFR3 confirmed Milroy disease in two unrelated patients. PMID: 25896638
    22. Case Reports: novel FLT4 gene mutation in a Chinese family with Milroy disease. PMID: 26714373
    23. TNFR1 has a role in mediating TNF-alpha-induced tumour lymphangiogenesis and metastasis by modulating VEGF-C-VEGFR3 signalling PMID: 25229256
    24. Experiments in mice and zebrafish demonstrate that changing levels of VEGFR3/Flt4 modulates aortic lumen diameter consistent with flow-dependent remodeling PMID: 25643397
    25. VEGFR-3 is a new target to improve net ultrafiltration in methylglyoxal-induced peritoneal injury by suppressing lymphatic absorption PMID: 26121315
    26. the best characterized of these signaling pathways, that involving the vascular endothelial growth factor (VEGF) family members VEGF-C and VEGF-D, together with their receptors VEGFR2 and VEGFR3. PMID: 25399804
    27. Although MYC is a valuable ancillary tool in distinguishing angiosarcomas from atypical vascular lesions , FLT4 immunohistochemistry may be used to screen for patients with FLT4 gene amplification PMID: 25864386
    28. Expression of VEGFR-3 was highly correlated with tumor metastasis in prostate cancer patients. PMID: 24858271
    29. Neuropilin-2 mediates lymphangiogenesis of colorectal carcinoma via a VEGFC/VEGFR3 independent signaling. PMID: 25543087
    30. High CD31 expression associated significantly with better survival and VEGFR3 had no association with survival. Both higher tumor grade and stage were associated with a decreased survival time PMID: 25667475
    31. analysis of how VEGF, VEGFR3, and PDGFRB protein expression is influenced by RAS mutations in medullary thyroid carcinoma PMID: 24754736
    32. VEGFR3 lymphatic endothelium signaling involves regulation of AKT activation via VEGFR3/VEGFR2/neuropilin 1 complex, ERK via VEGFR3/R3 homodimer, as well as regulatory roles of VE-PTP. PMID: 25524775
    33. increased expression in tumors of Ang-2 may individually, or in combination with VEGFR-3, predict poor prognosis of OSCC PMID: 24040410
    34. VEGF-C down-regulates VEGFR-3 in lymphatic endothelial cells PMID: 25281926
    35. Increase of VEGFR3 protein expression is associated with oral squamous cell carcinoma. PMID: 24085575
    36. Data suggest that VEGFC (vascular endothelial growth factor C) enhances cervical cancer invasiveness via up-regulation of galectin-3 via stimulation of NFkappaB/RELA pathway; galectin-3 interacts/activates VEGFR3. PMID: 24650367
    37. The expressions of VEGF-A, VEGFR2 and VEGFR3 were studied in by immunohistochemistry in 76 endometrial carcinoma specimens. VEGFR2 and VEGFR3 receptor expression were also studied by qRT-PCR in 17 tumors in comparison to normal endometrium. PMID: 24845798
    38. The present findings suggest the potential role of VEGF-C in the pathogenesis and development of a pterygium through lymphangiogenesis and the VEGF-C/VEGFR-3 pathway as a novel therapeutic target for the human pterygium. PMID: 22910845
    39. These findings suggest that the VEGFC/VEGFR3 pathway acts as an enhancer of ovarian cancer progression PMID: 24508126
    40. A novel GC-rich element (GRE) spanning -101/-66 sufficient for VEGFR3 transcription and activated by Sp1 and Sp3, respectively, was identified. PMID: 24710631
    41. Case Report: FLT4 missense mutation in Milroy disease. PMID: 25109169
    42. probe F2 facilitated the identification of the target spectrum of the two inhibitors confirming many of the previously identified (off-) targets such as AURKA, FLT4-VEGFR3, IKBKE and PDGFRbeta. PMID: 24184958
    43. The CXCL12-CXCR4 axis may influence the expression of VEGFR3 in urothelial bladder carcinoma and promote tumor recurrence. PMID: 24982366
    44. In primary ovarian cancer tissue, VEGFR3 expression, detected with an frequency of 26%, was mostly located in the vascular wall and across the stroma. PMID: 24713547
    45. VEGF-C and VEGFR-3 expression was significantly higher in luminal A subtype compared to luminal B. PMID: 24398987
    46. Transwell assays revealed that VEGF-C receptor, VEGFR-3, as well as chemokine CCL21 receptor, CC chemokine receptor 7 (CCR7), were responsible for the migration of PC3 cells toward hypoxia preconditioned MSCs PMID: 23939705
    47. Lymph node and lung metastases of HEC1A cells were completely suppressed by the muscle-mediated expression of sVEGFR-3. PMID: 23614535
    48. unlike an anti-VEGFR-3 Mab (mF4-31C1), DC101 was not capable of eliminating either tumor lymphangiogenesis or lymphogenous metastasis (60 % reduction of lymph node metastasis by DC101 vs 95 % by mF4-31C1). PMID: 23591595
    49. Data suggest that circulating VEGFR3/CD34 are biomarkers for epithelial ovarian cancer (EOC); circulating bone marrow-derived lymphatic/vascular endothelial progenitor cells are significantly increased in EOC and correlate with lymph node metastasis. PMID: 23803010
    50. Binding of VEGF-C and endostatin to recombinant VEGFR-3 is competitive. PMID: 22512651

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  • 相关疾病：
    Lymphedema, hereditary, 1A (LMPH1A); Hemangioma, capillary infantile (HCI)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Note=Ligand-mediated autophosphorylation leads to rapid internalization.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
  • 组织特异性：
    Detected in endothelial cells (at protein level). Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.
  • 数据库链接：

    HGNC: 3767

    OMIM: 136352

    KEGG: hsa:2324

    STRING: 9606.ENSP00000261937

    UniGene: Hs.646917