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  4. Human V-type proton ATPase 116 kDa subunit a isoform 2(ATP6V0A2) ELISA kit

Human V-type proton ATPase 116 kDa subunit a isoform 2(ATP6V0A2) ELISA kit

  • 中文名称：
    人V型质子ATP酶116kDa亚基α亚型3(ATP6V0A2)酶联免疫试剂盒
  • 货号：
    CSB-EL002386HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ATP6V0A2 ELISA Kit was designed for the quantitative measurement of Human ATP6V0A2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    a2 ELISA Kit; A2V ATPase ELISA Kit; ARCL ELISA Kit; ATP6a2 ELISA Kit; ATP6N1D ELISA Kit; ATP6V0A2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a isoform 2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a2 ELISA Kit; Infantile malignant osteopetrosis ELISA Kit; J6B7 ELISA Kit; Lysosomal H(+) transporting ATPase V0 subunit a2 ELISA Kit; Lysosomal H(+)-transporting ATPase V0 subunit a2 ELISA Kit; regeneration and tolerance factor ELISA Kit; Stv1 ELISA Kit; TJ6 ELISA Kit; TJ6M ELISA Kit; TJ6s ELISA Kit; V ATPase 116 kDa isoform a2 ELISA Kit; V type proton ATPase 116 kDa subunit a ELISA Kit; V type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; V-ATPase 116 kDa isoform a2 ELISA Kit; V-type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vph1 ELISA Kit; VPP2_HUMAN ELISA Kit; WSS ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ATP6V0A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of human ATP6V0A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.281 2.298 2.290 2.098  
    1000 1.615 1.642 1.629 1.437  
    500 0.995 0.954 0.975 0.783  
    250 0.634 0.626 0.630 0.438  
    125 0.468 0.449 0.459 0.267  
    62.5 0.358 0.337 0.348 0.156  
    31.25 0.287 0.298 0.293 0.101  
    0 0.191 0.193 0.192    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.
  • 基因功能参考文献：
    1. Data suggest that missense mutations in ATP6V0A2 and ATP6V0A4 that cause either cutis laxa or distal renal tubular acidosis result in enzyme subunits that are unstable, retained in endoplasmic reticulum (rather than transported to Golgi and cell membrane), and quickly degraded by proteasomes despite full glycosylation. PMID: 29311258
    2. Study shows how tumor associated a2-isoform V-ATPase can induce neutrophil migration by stimulating autocrine secretion of IL-8, suggesting a mechanism for the creation of a level of inflammation that favors cancer growth. PMID: 27845385
    3. In cisplatin resistant cells, shRNA mediated inhibition of V-ATPase-V0a2 enhanced sensitivity towards both cisplatin and carboplatin. PMID: 26899534
    4. a2V deficiency disrupts the endolysosomal route in Notch and TGF signaling, thereby impairing mammary gland development. PMID: 27809299
    5. Senescence-associated impaired expression of ATP6V0A2 triggers changes in Golgi structure and glycosylation in old fibroblasts, which demonstrates a role of ATP6V0A2 in cellular senescence program. PMID: 26611489
    6. the results from this study demonstrate that the a2-subunit isoform of Vacuolar ATPase regulates Notch signaling in breast tumor cells PMID: 26418877
    7. The granule-associated a2V isoform has a role in maintaining a pH gradient within the cell between the cytosol and granules in neutrophils. PMID: 25877929
    8. Case Report: novel ATP6V0A2 mutations in an infant with cutis laxa. PMID: 24815019
    9. Expression of a2 vacuolar ATPase in spermatozoa is associated with semen quality and chemokine-cytokine profiles in infertile men. PMID: 23936208
    10. Mutations in the ATP6V0A2 gene is associated with autosomal recessive cutis laxa. PMID: 22773132
    11. A mechanism is described by which tumor-associated macrophages mature via a nontraditional cytokine-like signal, the a2NTD peptide. PMID: 21178005
    12. Data show that the V-ATPase a2-subunit might actually be embedded into and/or closely associated with membrane phospholipids even in the absence of any obvious predicted transmembrane segments. PMID: 20669186
    13. Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO. PMID: 20153292
    14. Studies indicate that mutations in the ATP6V0A2 gene were found in families with autosomal recessive cutis laxa. PMID: 19401719
    15. RTF (Regeneration and tolerance factor), the alpha-2 isoform of the alpha subunit of vacuolar ATPase, has a role in controlling IL-1 beta secretion by regulating P2X7 activity. PMID: 15301855
    16. cells were not susceptible to apoptosis when the 70-kDa RTF was present but were when the 50-kDa RTF was present; the increase in levels of the 50-kDa RTF on cells from HIV-positive individuals is important in preventing apoptosis PMID: 15358640
    17. RTF is constitutively expressed at endometrial and decidual level, and its up-regulation during the secretory phase of the cycle may be relevant in mediating some immune-related aspects of uterine physiology. PMID: 15373763
    18. Its role in organellar proton pumping suggests that hTJ6 function may participate in protein trafficking/processing. PMID: 16113235
    19. Data suggest a role for the N-terminus domain of the a2 isoform of vacuolar ATPase in the regulation of IL-1beta pro-inflammatory cytokine production at the fetal-maternal interface. PMID: 17295899
    20. Study identified loss-of-function mutations in ATP6V0A2, encoding the a2 subunit of the V-type H+ ATPase, in several families with autosomal recessive cutis laxa type II or wrinkly skin syndrome. PMID: 18157129
    21. the relationship between ATP6V0A2 mutations, the glycosylation defect and the autosomal recessive cutis laxa type II phenotype is discussed [review] PMID: 19171192
    22. Loss-of-function mutations in ATP6V0A2 lead to tropoelastin aggregation in the Golgi and increased apoptosis of elastogenic cells. PMID: 19321599

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  • 相关疾病：
    Cutis laxa, autosomal recessive, 2A (ARCL2A); Wrinkly skin syndrome (WSS)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Endosome membrane.
  • 蛋白家族：
    V-ATPase 116 kDa subunit family
  • 数据库链接：

    HGNC: 18481

    OMIM: 219200

    KEGG: hsa:23545

    STRING: 9606.ENSP00000332247

    UniGene: Hs.25786