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  4. Human Tyrosine-protein kinase receptor UFO (AXL) ELISA Kit

Human Tyrosine-protein kinase receptor UFO (AXL) ELISA Kit

  • 中文名称：
    人酪氨酸蛋白激酶受体UFO(AXL)酶联免疫试剂盒
  • 货号：
    CSB-EL002476HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        AXL（AXL receptor tyrosine kinase）是一种跨膜酪氨酸激酶受体，参与肿瘤、免疫调节、血管生成等生物学过程。其高表达与多种肿瘤的发生和进展相关，是潜在的治疗靶点。AXL的含量在不同组织和细胞类型中有所不同。
        j9九游会登录入口首页生物所提供的Human Tyrosine-protein kinase receptor UFO (AXL) ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人 血清、血浆、组织匀浆样本中的AXL，其灵敏度为7.81 pg/mL，检测范围为31.25 pg/mL-2000 pg/mL。
     
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AXL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 84-95  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 90  
    Range % 86-95  
    1:8 Average % 95  
    Range % 85-100  
  • 回收率：
    The recovery of human AXL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 91-99  
    EDTA plasma (n=4) 95 87-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.232 2.257 2.245 2.075  
    1000 1.462 1.520 1.491 1.321  
    500 0.859 0.871 0.865 0.695  
    250 0.527 0.554 0.541 0.371  
    125 0.325 0.335 0.330 0.160  
    62.5 0.277 0.259 0.268 0.098  
    31.25 0.221 0.233 0.227 0.057  
    0 0.165 0.175 0.170 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    AXL是一种受体酪氨酸激酶，已被发现在多种癌症中起重要作用。最新的研究表明，AXL的过度表达可能导致肿瘤细胞的增殖、侵袭和耐药性增加，因此成为治疗癌症的重要靶点之一。此外，最新研究还发现，AXL还参与了免疫反应和神经元细胞的发育等生理过程。
  • 功能：
    Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.; (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.; (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
  • 相关疾病：
    AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thyroid tumorigenesis. Overexpression of AXL and its ligand was also detected in many other cancers such as myeloproliferative disorders, prostatic carcinoma cells, or breast cancer.
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily
  • 组织特异性：
    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 数据库链接：

    HGNC: 905

    OMIM: 109135

    KEGG: hsa:558

    STRING: 9606.ENSP00000301178

    UniGene: Hs.590970