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  4. Human Twisted gastrulation protein homolog 1(TWSG1) ELISA kit

Human Twisted gastrulation protein homolog 1(TWSG1) ELISA kit

  • 中文名称：
    人扭曲原肠胚蛋白同源物1(TWSG1)酶联免疫试剂盒
  • 货号：
    CSB-EL025361HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TWSG1 ELISA Kit was designed for the quantitative measurement of Human TWSG1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

  • 别名：
    PSEC0250 ELISA Kit; twisted gastrulation homolog 1 (Drosophila) ELISA Kit; TSG ELISA Kit; TSG1 ELISA Kit; Twisted gastrulation BMP signaling modulator 1 ELISA Kit; Twisted gastrulation homolog 1 ELISA Kit; Twisted gastrulation protein ELISA Kit; Twisted gastrulation protein homolog 1 ELISA Kit; TWSG1 ELISA Kit; TWSG1_HUMAN ELISA Kit
  • 缩写：
    TWSG1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TWSG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %86-96
    1:2Average %99
    Range %92-106
    1:4Average %89
    Range %83-94
    1:8Average %92
    Range %87-98
  • 回收率：
    The recovery of human TWSG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-99
    EDTA plasma (n=4)8480-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.557 2.765 2.661 2.542
    40001.816 1.902 1.859 1.740
    20001.103 1.189 1.146 1.027
    10000.707 0.714 0.711 0.592
    5000.405 0.374 0.390 0.271
    2500.312 0.309 0.311 0.192
    1250.205 0.201 0.203 0.084
    00.123 0.115 0.119 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development.
  • 基因功能参考文献：
    1. Findings indicate that twisted gastrulation homolog 1 (TWSG1) maybe serves as a tumor suppressor gene in gastric cancer and potential biomarker or therapeutic target for the diagnosis and treatment of gastric cancer. PMID: 29756996
    2. Tsg functions as a BMP-agonist by inducing conformational change in chordin making it more susceptible to tolloid cleavage and as a BMP-antagonist either independently or via a chordin-mediated mechanism. PMID: 26829466
    3. Data suggest CHRD (chordin) cleavage by tolloid-like metalloproteinases is insufficient to regulate BMP(bone morphogenetic protein)/BMPR signaling; CHRD fragments and TSG (twisted gastrulation protein) contribute to inhibition of BMP/BMPR signaling. PMID: 26517884
    4. these results suggest that BMPER and Tsg maintain a fine-tuned equilibrium that controls BMP pathway activity and is necessary for vascular cell homeostasis. PMID: 23641068
    5. mutations in the twisted gastrulation homolog 1 gene are neither a common direct cause nor a frequent modifying factor for human holoprosencephaly pathologies PMID: 21227728
    6. Tsg functions as an agonist synergizing with TGF-beta to inhibit T-cell activation. PMID: 17164348
    7. interferes with BMP-mediated hepcidin expression and may act with GDF15 to dysregulate iron homeostasis in thalassemia syndromes. PMID: 19414861

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Twisted gastrulation protein family
  • 数据库链接：

    HGNC: 12429

    OMIM: 605049

    KEGG: hsa:57045

    STRING: 9606.ENSP00000262120

    UniGene: Hs.514685