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  4. Human Tissue factor pathway inhibitor,TFPI ELISA Kit

Human Tissue factor pathway inhibitor,TFPI ELISA Kit

  • 中文名称：
    人组织因子途径抑制物(TFPI)酶联免疫试剂盒
  • 货号：
    CSB-E08260h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        TFPI，全称为Tissue Factor Pathway Inhibitor，是一种天然的抗凝血蛋白质。TFPI主要通过抑制凝血酶在TF-FVIIa复合物上的活性，从而抑制凝血级联反应的进行。此外，TFPI还能够抑制细胞外基质的降解、细胞增殖和炎症反应等生物学过程。在正常情况下，TFPI的含量比较低，通常在几ng/mL的水平范围内。
        j9九游会登录入口首页生物所提供的Human Tissue factor pathway inhibitor,TFPI ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的TFPI，其灵敏度为0.293 ng/mL，检测范围为0.782 ng/mL-50 ng/mL。
     
  • 别名：
    Anti convertin ELISA Kit; EPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; LACI ELISA Kit; Lipoprotein associated coagulation inhibitor ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; TFI ELISA Kit; TFPI 1 ELISA Kit; TFPI ELISA Kit; TFPI1 ELISA Kit; TFPI1_HUMAN ELISA Kit; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) ELISA Kit; Tissue factor pathway inhibitor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.782 ng/mL-50 ng/mL
  • 灵敏度：
    0.293 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-97  
    1:2 Average % 101  
    Range % 97-105  
    1:4 Average % 94  
    Range % 89-98  
    1:8 Average % 96  
    Range % 91-102  
  • 回收率：
    The recovery of human TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 86 81-92  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.519 2.623 2.571 2.440  
    25 2.021 2.154 2.088 1.957  
    12.5 1.434 1.456 1.445 1.314  
    6.25 0.813 0.838 0.826 0.695  
    3.125 0.436 0.445 0.441 0.310  
    1.563 0.304 0.310 0.307 0.176  
    0.782 0.221 0.216 0.219 0.088  
    0 0.130 0.131 0.131    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    TFPI是一种血管内皮细胞表达的抗凝血蛋白，可以通过抑制凝血酶的活性来防止血栓形成。近年来的研究表明，TFPI在多种疾病的发生和发展中起到重要作用，如心血管疾病、肺动脉栓塞和癌症等。
  • 功能：
    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 基因功能参考文献：
    1. plasma level not different between pre-eclampsia and normal pregnancy PMID: 28521572
    2. Patients with early onset preeclampsia are characterised by an attenuated coagulation response characterised by reduced thrombin generation stimulated by low-dose TF and elevated plasma TFPI activity. PMID: 28569919
    3. Developed functional protein S assays that measure both the activated protein C- and TFPI-cofactor activities of protein S in plasma, which are hardly if at all affected by the FV Leiden mutation. PMID: 28211163
    4. These data indicate that both isoforms of TFPI are present in advanced plaques and that anti-inflammatory M2 macrophages may be a potential source of TFPI. PMID: 28482260
    5. Among the various candidate genes involved in acute rejection, CD47 inhibits monocyte/macrophage-mediated phagocytosis by identifying the CD47 signal regulatory protein alpha (SIRP-alpha) as self/non-self. Tissue factor pathway inhibitor (TFPI) is involved in the regulation of the coagulation pathway and is able to bind to another ligand of CD47, thrombospondin-1 (TSP-1). PMID: 28393401
    6. Tissue Factor Pathway Inhibitor Gene Polymorphism is associated with response to therapy in Colorectal Cancer. PMID: 26968713
    7. For the first time, we show that a TFPI variant is significantly associated with fibrinogen levels and risk of coronary artery disease. PMID: 28894953
    8. miR-27a/b and miR-494 regulate TFPIalpha expression; there may be a role of these miRNAs in the estrogen-mediated downregulation of TFPIalpha PMID: 26999003
    9. Genetic variations of the TFPI genes seem to be related with coronary artery disease, which likely cooperate with metabolic risk factor (diabetes mellitus) and play critical roles in the pathogenesis of coronary artery disease in Han Chinese. PMID: 28716011
    10. Cleavage of FV at Arg(1545) , which abolishes the anticoagulant properties of FV and commits FV to the procoagulant pathway, is inhibited by binding of the TFPIalpha C-terminus to the FV acidic region PMID: 27801970
    11. The aim of this study was to evaluate the concentration of TF and its inhibitor TFPI in blood plasma, the impact of traditional and non-traditional cardiovascular risk factors on their concentration and the impact of both markers of haemostasis on the severity of subclinical atherosclerosis. PMID: 28749986
    12. The results show that cholesterol crystals induce TFPI and cytokine expression in M2-polarized macrophages through activation of the endoplasmic reticulum stress pathway and that TFPI has a protective effect against TNF-alpha and IL-6 mediated inflammation. PMID: 28712870
    13. Among high-risk women, the tissue factor pathway inhibitor activity of patients with gestational vascular complications is higher than that in other patients. Hence, these markers could augment a screening strategy. PMID: 28328938
    14. TFPI single nucleotide polymorphism is associated with venous thromboembolism. PMID: 28421636
    15. a concentration of tissue factor pathway inhibitor was significantly lower in patients with essential thrombocythemia with JAK2 V617F mutation as compared with patients without the mutation PMID: 26945263
    16. This review presents a comprehensive understanding of TFPI structure, biochemistry, and cellular expression which is necessary to understand how it modulates bleeding in hemophilia and the physiological impact of therapeutic agents targeting TFPI. PMID: 27207418
    17. the main finding of the current study is the remarkable elevation in the plasma levels of both total and free TFPI in septic patients at baseline. The levels of both forms of the inhibitor remained elevated throughout the first 24 h with further elevation after enoxaparin administration. PMID: 26377606
    18. TFPI-1 was a valuable predictor of deep venous thrombosis and tumor metastasis in non-small cell lung cancer patients. PMID: 28246607
    19. Circulating FVII, FVIIa and TFPI were significantly elevated in women with severe preeclampsia in the absence of comparable changes in plasma TF levels. PMID: 26765308
    20. HIF-1alpha is involved in the transcriptional regulation of the TFPI gene, and suggests that a hypoxic microenvironment inside a breast tumor may induce a procoagulant state in breast cancer patients. PMID: 26598923
    21. ERalpha can interact with all three ERE half-sites in the TFPI 5'-flanking region. PMID: 26999742
    22. rs5940 and rs7586970 moderate TFPI plasma levels and are candidate risk factors for thrombosis, but the regulation of TFPI plasma levels involves genetic factors beyond the TFPI gene. Review. PMID: 25879386
    23. Tissue factor pathway inhibitor activity and APC resistance are related to coronary heart disease risk in women, but may not explain the increased CHD risk due to estrogen plus progestin therapy. PMID: 26681757
    24. Association between genetic polymorphisms and deep vein thrombosis in a Chinese population. PMID: 26233570
    25. coagulation system, TFPI play an important role in the pathogenesis of CRSwNP. PMID: 26163243
    26. A significant degradation of TFPI was induced by ROS exposure, resulting in a decreased ability to bind FXa. ROS induce a procoagulant state in endothelial cells by altering TFPI structure, resulting in inhibition of TFPI binding to Factor Xa PMID: 25712553
    27. TNFalpha and Stx-1 compared to TNFalpha alone leads to an increase in procoagulant TF on endothelial cells, which is explained by a significant decrease in TFPI not co-localized with TF and not to an increase in TF not co-localized with TFPI PMID: 25864889
    28. Direct inhibition of FXa by TFPIalpha inhibits thrombin generation and contributes to the downregulation of coagulation. PMID: 25348176
    29. Tissue factor pathway inhibitor-2 gene polymorphisms were associated with coronary atherosclerosis in the Chinese population PMID: 26496276
    30. This study indicates that genetic and phenotypic variation of both TFPIalpha and TFPIbeta, more than TF, are markers of cancer progression. PMID: 25882602
    31. Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells PMID: 25617766
    32. Describe inhibition of tissue factor:factor VIIa-catalyzed factor IX and factor X activation by TFPI and TFPI constructs. PMID: 25163770
    33. Neither genetic polymorphisms nor the plasma levels of TFPI seem to act as direct risk factors for venous thromboembolism. PMID: 24448154
    34. Thrombin decreases TFPI expression in human pleural mesothelial cells. PMID: 25303460
    35. Colon TFPI levels are increased in ulcerative colitis. PMID: 24966616
    36. TFPI inhibits lectin pathway of complement activation by direct interaction with MASP-2. PMID: 25359215
    37. Most capillary endothelial cells of the cholecystitis group presented weak expression for TFPI. PMID: 24716194
    38. TFPI is an early biomarker in myocardial infarct and may explain the final infarct size. PMID: 24461489
    39. genetic polymorphism affects endogenous thrombin potential among FV Leiden carriers PMID: 24226152
    40. Data indicate that the protein S sex hormone-binding globulin (SHBG)-like domain was important for binding and enhancement of tissue factor pathway inhibitor (TFPI). PMID: 24740810
    41. Studies indicate differential expression of tissue factor pathway inhibitor isoforms TFPIalpha and TFPIbeta in platelets and endothelial cells. PMID: 24620349
    42. TFPI mRNA expression was increased in clear cell carcinoma (P<0.01). PMID: 24094893
    43. Both the protein S and the TFPI ratios were increased in patients with cirrhosis compared to controls. PMID: 23841464
    44. Small peptides blocking inhibition of factor Xa and tissue factor-factor VIIa by tissue factor pathway inhibitor (TFPI). PMID: 24275667
    45. Demonstrate a 5' untranslated region alternative splicing event that alters translation of TFPI isoforms produced via independent 3' splicing events within the same gene. PMID: 24233486
    46. PS exerts anticoagulant cofactor activity with TFPIalpha from any physiological pool, likely by localizing TFPIalpha to membrane surfaces, stabilizing its interaction with membrane-bound FXa, and slowing thrombin generation. PMID: 24233490
    47. Tissue factor pathway inhibitor-alpha inhibits prothrombinase during the initiation of blood coagulation. PMID: 24127605
    48. The east Texas bleeding disorder-associated F5(A2440G) mutations leads to the formation of the TFPIalpha:FV-short complex, which inhibits activation and propagation of coagulation. PMID: 23979162
    49. The effect of oral contraceptives on TFPI and PS is a possible explanation for the increased risk of venous thrombosis associated with oral contraceptives. PMID: 23407778
    50. tissue factor pathway inhibitor has a role in coagulation in hemostasis, particularly when FVIII is missing PMID: 23348798

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  • 亚细胞定位：
    [Isoform Alpha]: Secreted.; [Isoform Beta]: Microsome membrane; Lipid-anchor, GPI-anchor.
  • 组织特异性：
    Mostly in endothelial cells.
  • 数据库链接：

    HGNC: 11760

    OMIM: 152310

    KEGG: hsa:7035

    STRING: 9606.ENSP00000233156

    UniGene: Hs.516578