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  4. Human Thromboxane B2,TX-B2 ELISA Kit

Human Thromboxane B2,TX-B2 ELISA Kit

  • 中文名称：
    人血栓素B2(TXB2)酶联免疫试剂盒
  • 货号：
    CSB-E08046h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TX-B2 ELISA Kit was designed for the quantitative measurement of Human TX-B2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.38 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL .

  • 缩写：
    TX-B2
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    9.38 pg/mL-600 pg/mL
  • 灵敏度：
    2.34 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 80-105  
    1:2 Average % 95  
    Range % 85-108  
    1:4 Average % 106  
    Range % 90-112  
    1:8 Average % 95  
    Range % 80-110  
  • 回收率：
    The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 80-110  
    EDTA plasma (n=4) 98 90-108  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    600 3.147 3.121 3.134 2.997  
    300 2.534 2.521 2.528 2.390  
    150 1.750 1.744 1.747 1.610  
    75 1.052 1.046 1.049 0.912  
    37.5 0.659 0.644 0.652 0.514  
    18.75 0.443 0.428 0.436 0.298  
    9.37 0.263 0.284 0.274 0.136  
    0 0.134 0.141 0.138 　  
  • 数据处理：
  • 货期：
    3-5 working days

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