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  4. Human Thrombospondin type-1 domain-containing protein 4(THSD4) ELISA kit

Human Thrombospondin type-1 domain-containing protein 4(THSD4) ELISA kit

  • 中文名称：
    人血小板反应素1型结构域蛋白4(THSD4)酶联免疫试剂盒
  • 货号：
    CSB-EL023518HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human THSD4 ELISA Kit was designed for the quantitative measurement of Human THSD4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 别名：
    THSD4 ELISA Kit; UNQ9334/PRO34005 ELISA Kit; Thrombospondin type-1 domain-containing protein 4 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6 ELISA Kit; ADAMTS-like protein 6 ELISA Kit; ADAMTSL-6 ELISA Kit
  • 缩写：
    THSD4
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human THSD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 88  
    Range % 81-95  
    1:40 Average % 91  
    Range % 86-97  
    1:80 Average % 97  
    Range % 91-102  
    1:160 Average % 102  
    Range % 97-107  
  • 回收率：
    The recovery of human THSD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 103 95-110  
    EDTA plasma (n=4) 93 85-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    800 2.675 2.870 2.773 2.674  
    400 2.324 2.345 2.335 2.236  
    200 1.618 1.655 1.637 1.538  
    100 0.864 0.901 0.883 0.784  
    50 0.502 0.496 0.499 0.400  
    25 0.324 0.345 0.335 0.236  
    12.5 0.198 0.200 0.199 0.100  
    0 0.101 0.096 0.099 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN.
  • 基因功能参考文献：
    1. In breast cancer, some GATA3 effects shift from tumor suppressing to tumor promoting during tumorigenesis, with deregulation of three genes, BCL2, DACH1, THSD4, representing major GATA3-controlled processes in cancer progression. PMID: 25410484
    2. ADAMTSL6beta has a role in fibrillin-1 microfibril formation PMID: 21880733
    3. THSD4 polymorphism was not found to be associated with COPD risk PMID: 21965014
    4. Variations may be important determinants of osteoporosis in Japanese women. PMID: 18488137
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 数据库链接：

    HGNC: 25835

    OMIM: 614476

    KEGG: hsa:79875

    STRING: 9606.ENSP00000261862

    UniGene: Hs.387057