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Human Thrombospondin-4(THBS4) ELISA kit

  • 中文名称：
    人血小板反应蛋白4(THBS4)酶联免疫试剂盒
  • 货号：
    CSB-EL023490HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human THBS4 ELISA Kit was designed for the quantitative measurement of Human THBS4 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL .

  • 别名：
    Thbs4 ELISA Kit; thrombospondin 4 ELISA Kit; thrombospondin 4b ELISA Kit; Thrombospondin IV ELISA Kit; Thrombospondin-4 ELISA Kit; TSP4 ELISA Kit; TSP4_HUMAN ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human THBS4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %101
    Range %98-105
    1:4Average %99
    Range %95-104
    1:8Average %92
    Range %88-96
  • 回收率：
    The recovery of human THBS4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-99
    EDTA plasma (n=4)9389-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.673 2.698 2.686 2.539
    2502.034 2.086 2.060 1.913
    1251.242 1.268 1.255 1.108
    62.50.695 0.675 0.685 0.538
    31.20.403 0.415 0.409 0.262
    15.60.278 0.299 0.289 0.142
    7.80.217 0.227 0.222 0.075
    00.146 0.148 0.147 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.
  • 基因功能参考文献：
    1. establish a novel molecular mechanism by which miR-148a-3p upregulates Tsp-4 expression in tenocytes to promote angiogenesis by targeting KLF6, which could be helpful for the treatment of tendinopathy in the future PMID: 29807011
    2. Findings indicate that loss of miR-142 results in the over-expression of THBS4, which enhances HCC migration and vascular invasion. PMID: 28177895
    3. the present study demonstrates that THBS4 and lncRNA-THBS4-003 serve a significant role in PCa proliferation and migration via the MMP-9 and p38 MAPK signaling pathway. PMID: 27357608
    4. The THBS4 A387P polymorphism was associated with coronary artery disease in American population. PMID: 25976449
    5. Tsp4 functions as an ECM scaffold at myotendinous junctions, with potential therapeutic uses in tendon strengthening and repair PMID: 24941943
    6. THBS4 expression in breast cancer-associated extracellular matrix contributes to the activated stromal response exhibited during tumour progression PMID: 23942617
    7. the balancing selection target in THBS4 is likely represented by one or more variants that regulate tissue-specific and sex-specific gene expression PMID: 23420636
    8. The results of thid study suggested that injury-induced spinal TSP4 may contribute to spinal presynaptic hypersensitivity and neuropathic pain states. PMID: 22745497
    9. genetic polymorphism predicts cardiovascular risk in postinfarction patients with high HDL cholesterol and C-reactive protein levels PMID: 22011848
    10. Identify THBS4 as a powerful marker for diffuse-type gastric adenocarcinomas and to provide an initial characterization of its expression in the course of this disease. PMID: 21701537
    11. High THBS4 methylation resulting in inactivation of the gene is associated with colorectal cancer. PMID: 20846368
    12. thrombospondin-4 C-terminal peptide has a role in stimulating erythroid cell proliferation PMID: 15474480
    13. Promoter hypermethylation of thrombospondin 4 is associated with cutaneous T-cell lymphoma PMID: 15897551
    14. Homozygosity for thrombospondin-4 1186C (Arg387Pro) variant is a risk factor for myocardial infarction especially in older women. PMID: 16923428
    15. The functional analysis of the rat counterpart. PMID: 7490284

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  • 亚细胞定位：
    Endoplasmic reticulum. Sarcoplasmic reticulum. Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Thrombospondin family
  • 数据库链接：

    HGNC: 11788

    OMIM: 600715

    KEGG: hsa:7060

    STRING: 9606.ENSP00000339730

    UniGene: Hs.211426