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  4. Human Tenascin-N(TNN) ELISA kit

Human Tenascin-N(TNN) ELISA kit

  • 中文名称：
    人肌腱蛋白N(TNN)酶联免疫试剂盒
  • 货号：
    CSB-EL024008HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TNN ELISA Kit was designed for the quantitative measurement of Human TNN protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    TNN ELISA Kit; TNW ELISA Kit; Tenascin-N ELISA Kit; TN-N ELISA Kit; Tenascin-W ELISA Kit; TN-W ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %88-99
    1:2Average %102
    Range %97-105
    1:4Average %94
    Range %88-98
    1:8Average %98
    Range %93-102
  • 回收率：
    The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9286-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.022 2.001 2.012 1.855
    51.733 1.812 1.773 1.616
    2.51.307 1.288 1.298 1.141
    1.250.989 0.973 0.981 0.824
    0.6250.673 0.666 0.670 0.513
    0.3120.466 0.454 0.460 0.303
    0.1560.301 0.312 0.307 0.150
    00.156 0.158 0.157
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1. Involved in neurite outgrowth and cell migration in hippocampal explants. During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells. Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells.
  • 基因功能参考文献：
    1. This study demonstrated that the genetic defect was totally or partly clarified in 21 patients with nine of them having potential disease-causing mutations in TTN. PMID: 26627873
    2. Results show that tenascin-W acts as a niche component for breast cancer metastasis to bone by supporting cell migration and cell proliferation of the cancer cells. PMID: 25868708
    3. The expression of tenascin-W is dependent on p38MAPK and JNK signaling pathways in mammary tumors. (tenascin-W) PMID: 15592496
    4. data imply that tenascin-W expression in the activated tumor stroma facilitates tumorigenesis by supporting the migratory behavior of breast cancer cells PMID: 17909022
    5. results reveal a clear association between elevated levels of tenascin-W and the presence of colorectal cancer and breast cancer PMID: 18306355

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Tenascin family
  • 组织特异性：
    Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested. In brain tumors, detected around the endothelial cell layer of the clood vessel
  • 数据库链接：

    HGNC: 22942

    OMIM: 617472

    KEGG: hsa:63923

    STRING: 9606.ENSP00000239462

    UniGene: Hs.156369