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  4. Human TGF-beta-inducible early response gene-1,TIEG1 ELISA Kit

Human TGF-beta-inducible early response gene-1,TIEG1 ELISA Kit

  • 中文名称：
    人TGF-β诱导早期基因1(TIEG1)酶联免疫试剂盒
  • 货号：
    CSB-E09186h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human TIEG1 Elisa kit is suitable for the quantitative measurement of human TIEG1 in serum, plasma, tissue homogenates, or cell lysates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of human TIEG1 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

    TIEG1, also known as KLF10, is inducibly expressed by TGF-β, bone morphogenetic proteins (BMPs), and other cytokines. It is expressed in specific cell types in numerous tissues and is involved in inhibiting cell proliferation and inflammation as well as inducing apoptosis similar to that of TGFβ. TIEG1 binds to Sp-1-GC-rich DNA sequences and can activate or suppress the transcription of numerous genes. TIEG1 also plays an important role in regulating cell apoptosis and proliferation in various diseases, including pancreatic cancer, leukemia, and osteoporosis.

  • 别名：
    Early growth response; alpha ELISA Kit; Early growth response; alpha like ELISA Kit; EGR A ELISA Kit; EGR alpha ELISA Kit; EGR(A) ELISA Kit; EGR-alpha ELISA Kit; EGRA ELISA Kit; Egral ELISA Kit; EGRalpha ELISA Kit; Gdnfif ELISA Kit; KLF 10 ELISA Kit; KLF10 ELISA Kit; KLF10_HUMAN ELISA Kit; Krueppel like factor 10 ELISA Kit; Krueppel like factor10 ELISA Kit; Krueppel-like factor 10 ELISA Kit; Kruppel like factor 10 ELISA Kit; mGIF ELISA Kit; TGF-beta- inducible early gene ELISA Kit; TGFB inducible early growth response 1 ELISA Kit; TGFB inducible early growth response ELISA Kit; TGFB inducible early growth response protein 1 ELISA Kit; TGFB-inducible early growth response protein 1 ELISA Kit; TIEG 1 ELISA Kit; TIEG ELISA Kit; TIEG-1 ELISA Kit; TIEG1 ELISA Kit; Transforming growth factor beta inducible early growth response ELISA Kit; Transforming growth factor beta inducible early growth response protein 1 ELISA Kit; Transforming growth factor-beta-inducible early growth response protein 1 ELISA Kit; Zinc finger transcription factor TIEG ELISA Kit
  • 缩写：
    KLF10
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    34.38 pg/mL-2200 pg/mL
  • 灵敏度：
    8.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TIEG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 81-89
    1:2 Average % 93
    Range % 90-96
    1:4 Average % 84
    Range % 81-86
    1:8 Average % 95
    Range % 91-99
  • 回收率：
    The recovery of human TIEG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 100-105
    EDTA plasma (n=4) 98 94-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2200 2.435 2.431 2.433 2.310
    1100 1.708 1.687 1.698 1.575
    550 1.020 1.000 1.010 0.887
    275 0.533 0.570 0.552 0.429
    137.5 0.361 0.358 0.360 0.237
    68.75 0.291 0.287 0.289 0.166
    34.38 0.203 0.212 0.208 0.085
    0 0.122 0.124 0.123 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis. May play a role in the cell cycle regulation.
  • 基因功能参考文献：
    1. Results from a study on gene expression variability markers in early-stage human embryos shows that KLF10 is a putative marker for the 3-day, 8-cell embryo stage. PMID: 26288249
    2. Zinc is capable of ameliorating the allogeneic immune reaction by enhancement of antigen-specific iTreg cells due to modulation of essential molecular targets by upregulation of Foxp3 and KLF-10 and downregulation of IRF-1. PMID: 27260002
    3. Thus, our study uncovers a novel regulatory mechanism underlying which KLF10 stability and its biological function are mediated by FBW7. PMID: 29198712
    4. In clinical specimens of lung adenocarcinoma, low KLF10 expression associated with decreased patient survival, consistent with a pivotal role for KLF10 in distinguishing the antiproliferative versus prometastatic functions of TGFbeta. Our results establish that KLF10 functions to suppress TGFb-induced EMT, establishing a molecular basis for the dichotomy of TGFb function during tumor progression. PMID: 28249899
    5. the findings show that TIEG1 is highly expressed in human keloids and that it directly binds and represses Smad7 promoter-mediated activation of TGF-beta/Smad2 signaling PMID: 28108300
    6. photoexposure decreases expression in fibroblasts and keratinocytes PMID: 26119394
    7. TIEG-1 overexpression in normal human skin fibroblasts results in improved resistance to oxidative stress PMID: 26922828
    8. CDK2 up-regulates the protein level of KLF10 through reducing its association with SIAH1, a KLF10 E3-ubiqutin ligase involved in proteasomal degradation. PMID: 25728284
    9. KLF10 directly binds to TGF-BRII promoter in T cells, leading to enhanced gene expression. PMID: 25472963
    10. The tumor suppressor function of LSAMP is most likely exerted by reducing the proliferation rate of the tumor cells, possibly by indirectly upregulating one or more of the genes HES1, CTAG2 or KLF10. PMID: 24885297
    11. KLF10, functions as a toggle to integrate antagonistic signals regulating FOXP3 via Sin3-HDAC/PCAF pathway and, thus, immune activation. PMID: 24944246
    12. RAF-1 phosphorylation and PIN1 isomerization together regulate KLF10 stability and further affect the role of KLF10 in tumor progression. PMID: 23994618
    13. KLF10 is an effective repressor of myoblast proliferation and represses FGFR1 promoter activity in these cells via an Sp1 binding site. PMID: 23569208
    14. KLF10 may constitute a pharmacogenomic marker to discriminate between response and nonresponse to hydroxyurea treatment in beta-hemoglobinopathy patients. PMID: 23057549
    15. KLF10 is a potential clinical predictor for progression of pancreatic cancer. PMID: 22688058
    16. study identified 6 novel, hypertrophic cardiomyopathy associated TIEG1 missense variants and have demonstrated that a number of these variants have abnormal function with regard to mutant TIEG1's ability to regulate either the PTTG1 or SMAD7 promoters PMID: 22234868
    17. TIEG1 inhibits breast cancer cell invasion by inhibition of the EGFR signaling pathway. PMID: 22025675
    18. TIEG1 has a role in regulating the expression and activity of Runx2 in osteoblasts PMID: 21559363
    19. KLF10 is upregulated in human PDLCs subjected to tensile stress related to mechano-induced cell cycle arrest. PMID: 20934684
    20. Results indicate that HPV-16 oncoprotein E7 binds to the C-terminus of TIEG1 and induces its degradation via the ubiquitin pathway. E7 not only increased the ubiquitination of TIEG1 but also influenced the ability of TIEG1 to affect apoptosis. PMID: 20691807
    21. JARID1B is the first TIEG1 corepressor identified, explaining how TIEG1 represses transcription through inducing histone H3 lysine 4 demethylation, which may be important for TIEG1 function in both normal and cancer cells. PMID: 20863814
    22. KLF10 has been shown to play a major role in the TGFbeta inhibition of cell proliferation and inflammation and induction of apoptosis, and its overexpression in human osteoblasts and pancreatic carcinoma cells mimics the actions of TGFbeta[review] PMID: 20087894
    23. no sequence alterations or alterations in expression were found in TIEG1 in pancreatic cancer; an essential role of TIEG1 in pancreatic cancer can be excluded PMID: 12065093
    24. TIEG amplies Smad signaling. PMID: 12173049
    25. Up-regulation of CD11d expression following differentiation of myeloid cells is mediated through increased binding of TIEG1 binding to the CD11d promoter. PMID: 15087465
    26. Differential gene expression of TIEG, a tumor suppressor gene, plays a significant role in the proliferation of breast cancer PMID: 15218362
    27. Expression of TIEG is known to inhibit Smad7 expression in bronchial epithelial cells. PMID: 17377371
    28. TIEG1 induces apoptosis through mitochondrial apoptotic pathway and promotes apoptosis induced by homoharringtonine and velcade. PMID: 17659279
    29. Gene variants may weakly contribute to a particular genetic background that increases the susceptibility to development of type 2 diabetes. PMID: 17931948
    30. TIEG is rapidly induced in response to estrogen in osteoblasts by ERbeta, but not ERalpha PMID: 18483178
    31. Data suggest that TGFbeta and BMP-6 in the bone marrow microenvironment allow leukemia cells to escape therapy. Further, the data indicate that TIEG1 might be involved in mediating this effect from the microenvironment onto the leukemia cells. PMID: 18798273

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Sp1 C2H2-type zinc-finger protein family
  • 数据库链接：

    HGNC: 11810

    OMIM: 601878

    KEGG: hsa:7071

    STRING: 9606.ENSP00000285407

    UniGene: Hs.435001