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  4. Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

  • 中文名称：
    人小而密低密度脂蛋白（sLDL）酶联免疫试剂盒
  • 货号：
    CSB-E13350h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human sLDL ELISA Kit was designed for the quantitative measurement of Human sLDL protein in serum, plasma, saliva, urine, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 nmol/mL-20 nmol/mL and the sensitivity is 0.078 nmol/mL.

  • 缩写：
    sLDL
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, saliva, urine, tissue homogenates
  • 检测范围：
    0.312 nmol/mL-20 nmol/mL
  • 灵敏度：
    0.078 nmol/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human sLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 92-103  
    1:200 Average % 92  
    Range % 86-96  
    1:400 Average % 104  
    Range % 97-107  
    1:800 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human sLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 83-93  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/ml OD1 OD2 Average    
    20 0.086 0.085 0.086    
    10 0.177 0.181 0.179    
    5 0.384 0.375 0.380    
    2.5 0.718 0.704 0.711    
    1.25 1.253 1.269 1.261    
    0.625 1.728 1.714 1.721    
    0.312 2.223 2.121 2.172    
    0 2.526 2.425 2.476    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

What kind of dsLDL does the kit measure? (LDL-cholesterol? LDL-ApoB…. Etc?)
How does the kit differentiate de sdLDL from other LDL?

A:
Thanks for your inquiry.
This kit specifically use the sLDL raw material and detects the total sLDL(including the free SLDL or in combination with other proteins).
sLDL is the small dense Low-Density Lipoprotein,sd LDL or sLDL, which has much smaller grains and lower densities.
While those larger grains and lower densities LDL called large LDL.
You also can check more detailed informaiton on sLDL from this link: https://www.sciencedirect.com/science/article/pii/S0167527399001072 Pls let me know if you have any further questions. Thank you.