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  4. Human Serum amyloid A,SAA ELISA Kit

Human Serum amyloid A,SAA ELISA Kit

  • 中文名称：
    人血清淀粉样蛋白A(SAA)酶联免疫试剂盒
  • 货号：
    CSB-E08589h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SAA1 ELISA Kit was designed for the quantitative measurement of Human SAA1 protein in serum, urine. It is a Sandwich ELISA kit, its detection range is 156 ng/mL-10000 ng/mL and the sensitivity is 39 ng/mL.

  • 别名：
    SAA1 ELISA Kit; Serum amyloid A-1 protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA); Serum amyloid protein A(2-104); Serum amyloid protein A(3-104); Serum amyloid protein A(2-103); Serum amyloid protein A(2-102); Serum amyloid protein A(4-101)] ELISA Kit
  • 缩写：
    SAA1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine
  • 检测范围：
    156 ng/mL-10000 ng/mL
  • 灵敏度：
    39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %101
    Range %90-110
    1:2Average %95
    Range %91-98
    1:4Average %100
    Range %92-105
    1:8Average %89
    Range %86-96
  • 回收率：
    The recovery of human SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    100002.721 2.598 2.660 2.541
    50002.187 2.087 2.137 2.018
    25001.542 1.424 1.483 1.364
    12500.962 0.941 0.952 0.833
    6250.579 0.547 0.563 0.444
    312.50.387 0.371 0.379 0.260
    1560.263 0.259 0.261 0.142
    00.122 0.116 0.119 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human SAA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled SAA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Major acute phase protein.
  • 基因功能参考文献：
    1. SAA1 can be produced in human fetal membranes, which can be greatly induced in the presence of proinflammatory cytokines and glucocorticoids thereby producing effects associated with parturition PMID: 28386088
    2. High SAA1 expression in the stromal component is associated with pancreatic tumors. PMID: 29351990
    3. Data suggest that anti-inflammatory capacity of HDL (high-density lipoprotein) from patients with type 2 diabetes and diabetic nephropathy is impaired; this effect might be attributable to SAA enrichment in HDL in these patients; up-regulation of SAA in HDL appears to be associated with increased risk of diabetic angiopathy or vasculitis in such patients. PMID: 28760652
    4. The frequencies of the CC genotype and the C allele of SAA rs12218 were higher in participants with ischemic stroke than in the control group. The frequencies of the AG genotype and the G allele of rs2468844 were higher in participants with ischemic stroke than in the control group. Multiple logistic regression analysis revealed the significance of the rs12218 in males and in large-artery atherosclerosis group. PMID: 28870546
    5. we show that astrocytoma patients have increased levels of serum SAA and SAA1 is expressed and secreted in glioblastoma, and its co-expression with tumor-related genes supports its involvement in glioblastoma angiogenesis and progression. PMID: 28283801
    6. These results suggested that the C-terminal truncation of human SAA accelerates amyloid fibril formation. PMID: 29288051
    7. SAA1 can be a potential mediator for UV-induced MMP-1 expression in human skin. PMID: 26900010
    8. Data suggest that serum amyloid A (SAA) immunoreactivity in tumor-associated macrophage (TAM) is associated with worse recurrence-free survival, and is perhaps a therapeutic target for breast cancer. PMID: 27058895
    9. Findings indicate that in Lyme disease patients, circulating CRP and SAA (Serum Amyloid A) levels are highest when the concentration of spirochetes is greatest in skin and/or blood and that levels decline after the dissemination of the organism to extracutaneous sites in subsequent stages of infection. PMID: 27585799
    10. The Serum amyloid A (SAA)/formyl peptide receptor-like 1 (FPRL1) contributed to pathogenesis of psoriasis by promoting keratinocyte proliferation and inflammation, thus providing a potential therapeutic target for disease therapy. PMID: 27910163
    11. High SAA expression is associated with lung cancer. PMID: 27809798
    12. SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB PMID: 28278182
    13. Novel truncated form of serum amyloid A is elevated in the plasma of Kawasaki disease (KD) when compared with Febrile control subjects. Future studies will evaluate its relevance as a diagnostic biomarker and its potential role in the pathophysiology of KD. PMID: 27271757
    14. SR-A1 suppresses lung cancer metastasis by downregulating SAA1 production in tumor-associated macrophages (TAM). PMID: 28202524
    15. The SAA1.1 allele was found in four familial Mediterranean fever patients, including two with AA amyloidosis. PMID: 27150194
    16. genetic variants at the CRP and SAA1 loci independently affect both CRP and SAA levels, and their respective circulating levels act as suppressors. PMID: 27313400
    17. Serum amyloid A could represent a novel marker of primary unexplained recurrent early pregnancy loss because affected women exhibited elevated levels of this protein. PMID: 28099717
    18. The polymorphism of SAA1 is not associated with susceptibility and severity of Familial Mediterranean Fever in Egyptian children PMID: 27300189
    19. The findings identify SR-BII as a functional SAA receptor that mediates SAA uptake and contributes to its proinflammatory signaling via the MAPK-mediated signaling pathways. PMID: 28423002
    20. Correlate MEFV genotype and the SAA1 polymorphisms with the clinical manifestations of familial Mediterranean fever and the occurrence of amyloidosis in a large cohort of Armenian patients. PMID: 27791951
    21. Study found increased SAA concentration in patients with sarcoidosis. PMID: 26919159
    22. the detection of residues 76 and 77 of SAA (AA76) may alter the ability to diagnose AA amyloidosis. PMID: 27098620
    23. The results demonstrated that SAA upregulated Visfatin expression in cultured RAW264.7 macrophages and in the primary monocytes. PMID: 27006946
    24. Structure, function and SAA1 gene polymorphisms PMID: 26945629
    25. Serum Amyloid A induces inflammation, proliferation and cell death in activated hepatic stellate cells. PMID: 26937641
    26. Amyloid A overexpressed in renal cell carcinoma patients and can serve as a prognostic marker. PMID: 26750935
    27. incorporation of SAA into HDL preparations reduced antiinflammatory properties but not to the same extent as HDL from AgNO3-injected mice PMID: 26642365
    28. human SAA is possibly only expressed in a subset of septic patients; SAA induces HMGB1 release via TLR4 and RAGE receptors; SAA supplementation worsens the outcome of lethal endotoxemia PMID: 26052716
    29. The SAA1 rs12218 polymorphism was significantly more prevalent in ankylosing spondylitis patients with amyloidosis. PMID: 26300108
    30. Autocrine/paracrine and recombinant human SAA1 via TLR4 stimulate a proinflammatory phenotype that is both part of the early phase of osteogenic differentiation and the development of senescence. PMID: 26135899
    31. Serum amyloid A1alpha induces paracrine IL-8/CXCL8 via TLR2 and directly synergizes with this chemokine via CXCR2 and formyl peptide receptor 2 to recruit neutrophils. PMID: 26297794
    32. These functional properties set SAA apart from well-characterized inflammatory factors, such as LPS and TNF-alpha, suggesting that it may play a homeostatic role during the course of inflammation. PMID: 26130702
    33. The detailed analysis of the docking results points to the fact that the best serum amyloid A-Cystatin C (SAA-hCC) binding is achieved by the peptides inclined toward interacting via Lys90 and Arg 96 (with ffhCC Ser98 and Tyr102, respectively). PMID: 25736604
    34. Our results showed PCT as a valuable marker of bacterial infections in febrile patients. PCT was superior to CRP, IL-6 or SAA in the early identification of bacterial infection PMID: 25963492
    35. Mutations in genetic biomarker SAA1 gene related to amyloidosis processes may play a crucial role in chronic renal failure patients who require long-term hemodialysis. PMID: 25394530
    36. The relative abundance of the N-terminal arginine truncation of SAA1.1 is significantly decreased in diabetes and negatively correlates with measures of glycemic and lipid control. PMID: 25607823
    37. potential marker for detecting the activity of srcoidosis PMID: 25623898
    38. Genetic polymorphisms in SAA1 are associated with coronary artery disease in the Han and Uygur populations in Western China. PMID: 25656165
    39. High SAA serum level is associated with hepatocellular carcinoma. PMID: 25605163
    40. HDL3 subpopulations in ST segment elevation myocardial infarction were enriched 10 times in SAA patients. PMID: 26037829
    41. SAA has a role in activity of rheumatoid arthritis and risk of cardiovascular and renal involvement PMID: 25525305
    42. SR-B1 and p38 MAPK are involved in signaling pathway of serum amyloid A-induced angiogenesis in rheumatoid arthritis. PMID: 25932604
    43. Although SAA concentrations were elevated in the sera, there was no significant difference in these concentrations between familial Mediterranean fever patients and rheumatoid arthritis patients. PMID: 25240611
    44. Zn deficiency enhances the acute phase response and particularly the JAK-STAT3 pathway, resulting in increased serum amyloid A production. PMID: 24732911
    45. Serum amyloid A is a retinol binding protein that transports retinol during bacterial infection. PMID: 25073702
    46. Data suggest that serum amyloid A (SAA) is a candidate for diabetic kidney disease therapeutic and biomarker discovery. PMID: 25531567
    47. The CSF biomarker amyloid 1-42 is important and useful as part of the diagnostic procedure for detecting AD and other dementia in elderly patients displaying psychotic symptoms. PMID: 23597931
    48. homozygous SAA1.5/1.5 genotype appears to be a recessive susceptibility gene, which has lost the antiangiogenic function, whereas SAA1.1 and SAA1.3 are the dominant alleles of the tumor suppressor phenotype. PMID: 24608426
    49. keratinocyte-derived SAA triggers a key inflammatory mediator, IL-1beta, via NLRP3 inflammasome activation, providing new potential targets for the treatment of this chronic skin disease. PMID: 25231464
    50. SAA1 gene expression in COPD patients is responsible for the secretion of this molecule in different cell types. PMID: 24884805

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  • 相关疾病：
    Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    SAA family
  • 组织特异性：
    Expressed by the liver; secreted in plasma (at protein level).
  • 数据库链接：

    HGNC: 10513

    OMIM: 104750

    KEGG: hsa:6288

    STRING: 9606.ENSP00000348918

    UniGene: Hs.632144