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  4. Human Serpin B5(SERPINB5) ELISA kit

Human Serpin B5(SERPINB5) ELISA kit

  • 中文名称：
    人丝氨酸蛋白酶抑制剂B5(SERPINB5) 酶联免疫试剂盒
  • 货号：
    CSB-EL021073HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SERPINB5 ELISA Kit was designed for the quantitative measurement of Human SERPINB5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 别名：
    Maspin ELISA Kit; Ovalbumin ELISA Kit; Peptidase inhibitor 5 ELISA Kit; PI-5 ELISA Kit; PI5 ELISA Kit; protease inhibitor 5 (maspin) ELISA Kit; Protease inhibitor 5 ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade B; member 5 ELISA Kit; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 5 ELISA Kit; Serpin B5 ELISA Kit; serpin family B member 5 ELISA Kit; Serpin peptidase inhibitor ELISA Kit; Serpin peptidase inhibitor; clade B (ovalbumin); member 5 ELISA Kit; Serpinb5 ELISA Kit; SPB5_HUMAN ELISA Kit; Spi5 ELISA Kit; Spi7 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %86-93
    1:2Average %100
    Range %97-102
    1:4Average %94
    Range %89-97
    1:8Average %97
    Range %91-100
  • 回收率：
    The recovery of human SERPINB5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9790-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.286 2.226 2.256 2.074
    51.876 1.846 1.861 1.679
    2.51.562 1.550 1.556 1.374
    1.251.157 1.142 1.150 0.968
    0.6250.718 0.729 0.724 0.542
    0.3120.480 0.498 0.489 0.307
    0.1560.318 0.324 0.321 0.139
    00.181 0.183 0.182 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • 基因功能参考文献：
    1. data suggest that maspin inhibits high glucose-induced proliferation, oxidative stress, and angiogenesis of HRMECs at least by modulating the PI3K/AKT pathway. Maspin may be a potential therapeutic agent for the prevention and treatment of proliferative diabetic retinopathy. PMID: 29363056
    2. SERPINB5 may play an important role in rectal cancer progression and response to neoadjuvant CCRT and serve as a novel prognostic factor. PMID: 29511373
    3. maspin expression has a prognostic role in breast cancer. Maspin expression is related to increased angiogenesis. Subcellular localization of maspin can strongly affect cancer prognosis. Cytoplasmic maspin relates to poor prognostic parameters whereas nuclear maspin relates to good prognostic ones. PMID: 29126758
    4. Maspin nuclear expression in colorectal carcinoma buds may be helpful for a proper budding assessment and may serve as a negative prognostic factor. PMID: 28780084
    5. The expression of maspin in the cytoplasm alone could be useful for predicting unfavorable prognoses in patients with p-stage IA lung adenocarcinoma PMID: 29715131
    6. The severity of hip OA can be related to angiogenesis pathways that are not maspin-mediated. PMID: 27704157
    7. SNPs affected SERPINB5 expression and protein stability, which significantly correlated with tumour expression and subsequently with tumour development and aggressiveness. Taken together, our findings regarding these biomarkers provide a prediction model for risk assessment. PMID: 27221742
    8. Maspin is spontaneously secreted as an exosomal protein to regulate tumor/stromal interactions. PMID: 28009978
    9. Study results provide new insights into the molecular mechanisms of maspin suppression in response to HBx, and revealed nuclear IKKalpha as a prognostic biomarker and a potential therapeutic target to improve the clinical outcome of HBV-associated HCC patients. PMID: 27409165
    10. Mechanistic investigations found that quercetin suppressed Snail-dependent Akt activation by upregulating maspin and Snail-independent a disintegrin and metalloproteinase (ADAM) 9 expression pathways to modulate the invasive ability of NSCLC cells. PMID: 28648644
    11. High HDAC1 expression may contribute to the aggressiveness of human breast cancer with cytoplasmic-only expression of maspin PMID: 28870936
    12. These results reveal a novel biological function of Maspin in modulating macrophage activity PMID: 28064070
    13. Heterozygous TC of the SERPINB5 rs17071138 polymorphism may be a factor that increases susceptibility to oral cancer. PMID: 28339463
    14. Impact of Maspin Polymorphism rs2289520 G/C and Its Interaction with Gene to Gene, Alcohol Consumption Increase Susceptibility to Oral Cancer Occurrence PMID: 27525723
    15. Cytoplasmic expression of maspin could be an independent unfavourable prognostic indicator in patients with lung squamous cell carcinoma (SCC). PMID: 27297724
    16. Results demonstrated that Maspin suppressed growth, proliferation and invasion by delaying cell cycle transition and promoting apoptosis in cutaneous squamous cell carcinoma cells. PMID: 28405681
    17. This is the first time that these parts of maspin have been highlighted as having key roles affecting cell function PMID: 27888098
    18. In GC with associated metaplasia, cytoplasmic maspin is predominant; the nuclear shift induces local aggressiveness and risk of node metastases, whereas total loss can indicate a risk of distant metastases PMID: 26067133
    19. maspin mRNA is significantly up-regulated in tissues of pulmonary adenocarcinoma patients PMID: 26757744
    20. The data suggest that methylation-induced silencing of maspin contributes to the proliferation of human glioma cells, and maspin may be a potential therapeutic target in glioma. PMID: 27177016
    21. epithelial cytoplasmic expression of maspin and CD138 may have a significant role in tumorigenesis in ovarian high-grade serous carcinomas and clear cell carcinomas; these markers may regulate tumor cell proliferation, and their significant correlation to each other may suggest that CD138 probably induces maspin expression to protect tumor growth factors from being lysed by proteolytic enzymes PMID: 26526579
    22. Compared with the control group, maspin expression was down-regulated in the fungal rhinosinusitis group, and lower in the invasive fungal rhinosinusits group compared to the noninvasive fungal rhinosinusitis group. PMID: 28031066
    23. maspin may mediate the therapeutic efficacy of gemcitabine in hormone-independent prostate cancer. PMID: 26490978
    24. High CXCR4 expression in primary breast tumors (PTs) was found to be associated with luminal A type tumor, suggesting more favorable outcome. In contrast, CCR7 and FOXP3 expressions in PTs represented luminal B tumors, pointing to more aggressive tumor behavior. Maspin expression did not differ between luminal types. PMID: 28011488
    25. Data suggest that serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 protein (SERPINB5) seems to assume an oncogenic role in pancreatic ductal adenocarcinoma (PDAC). PMID: 26646275
    26. The report reveals an association between maspin expression and the prognosis of patients with soft tissue sarcomas (STS). PMID: 25358722
    27. Study shows that Maspin could enhance cisplatin chemosensitivity induced by cell apoptosis in muscle invasive bladder cancer (MIBC) cell lines. Its expression correlated with prognosis of MIBC patients who received cisplatin-based chemotherapy. PMID: 26733306
    28. Suggest maspin as immunohistochemical marker anticipating survival in non-small cell lung carcinomas. PMID: 26456962
    29. In this review, we discuss the maspin and maspin/HDAC1 interplay in tumor biology and immunology--{REVIEW} PMID: 26614844
    30. Results suggest insight into the dynamics of maspin as a metastasis suppressor gene for further molecular research. PMID: 26497917
    31. The expression of maspin in the A549 and SPCA1 cells was hardly detected. PMID: 26329803
    32. Intensity of maspin expression is more in benign tumors when compared with malignant tumors. The benign tumors showed both nuclear and cytoplasmic expression. Some malignant tumors did express maspin, but mainly in the cytoplasm PMID: 25098993
    33. Data suggest that hepatitis B virus X protein (HBx) induces gene expressions of microRNAs microRNA-7, -21, and -107 to target the 3' untranslational region (3' UTR) of maspin mRNA. PMID: 26296971
    34. The methylation status of the maspin promoter is an important factor that affects the migration and invasion of EVT cells during early pregnancy. PMID: 26263377
    35. Maspin protein expression is related to the carcinogenesis of oral cavity squamous cell carcinoma. PMID: 24801268
    36. The expression levels of maspin were lower in the cisplatin-resistant tissue and cells, and was enhanced by IGFBP-2 inhibition. PMID: 26080829
    37. maspin is a useful addition to the diagnostic immunohistochemical panel (S100P, pVHL, and insulin-like growth factor II mRNA-binding protein 3) to help distinguish malignant from benign epithelia on challenging bile duct biopsies. PMID: 26362203
    38. VEGF-C inhibition reverses resistance of bladder cancer cells to cisplatin via upregulating maspin. PMID: 25936422
    39. Maspin levels are elevated in colonic polyps. PMID: 25977386
    40. Report endothelial cell pathology and maspin expression in liposarcomas versus non-lipomatous soft tissue tumors. PMID: 25973067
    41. Nuclear maspin expression is associated with CPG island methylator phenotype, and clinicopathologically correlates with tumor aggressiveness in colorectal cancer. PMID: 25973084
    42. Depending on maspin expression, tumor cells in suspension and 3D collagen I show the phenotypes of stem-like and dormant tumor cell populations, respectively. Maspin reduces prostate tumor cell plasticity and enhances tumor sensitivity to salinomycin. PMID: 26208903
    43. Cytoplasmic maspin expression could be an independent poor prognostic indicator of patients with lung adenocarcinoma measuring <3 cm. PMID: 25322663
    44. Maspin expression in laryngeal carcinoma is a good indicator for postoperative adjuvant radiotherapy treatment results. PMID: 25459315
    45. Unmethylated-maspin DNA in maternal blood is associated with severe preeclampsia. PMID: 26095742
    46. U-SERPINB5 and M-SERPINB5 concentrations in maternal plasma during early pregnancy are not associated with pregnancies who delivered small for gestational age neonates. PMID: 25553975
    47. Maspin was not expressed in the normal mucosa. PMID: 26124368
    48. Tissue array analysis suggests role for maspin in regulating the occurrence of glioma and disease progression. PMID: 26051724
    49. These data show that changes of expression and subcellular localization of maspin may constitute an important biological end point of tumour progression and aid in the classification of lung adenocarcinoma. PMID: 25040445
    50. Hypomethylation of the maspin promoter results in increased expression of maspin in preeclamptic placentas. PMID: 25151033

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Serpin family, Ov-serpin subfamily
  • 组织特异性：
    Normal mammary epithelial cells.
  • 数据库链接：

    HGNC: 8949

    OMIM: 154790

    KEGG: hsa:5268

    STRING: 9606.ENSP00000372221

    UniGene: Hs.55279