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  4. Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

  • 中文名称：
    人丝氨酸/苏氨酸蛋白激酶LMTK3(LMTK3)酶联免疫试剂盒
  • 货号：
    CSB-EL013017HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LMTK3 ELISA Kit was designed for the quantitative measurement of Human LMTK3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

  • 别名：
    LMTK3 ELISA Kit; KIAA1883 ELISA Kit; TYKLM3 ELISA Kit; Serine/threonine-protein kinase LMTK3 ELISA Kit; EC 2.7.11.1 ELISA Kit; Lemur tyrosine kinase 3 ELISA Kit
  • 缩写：
    LMTK3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.7 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LMTK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-94
    1:2 Average % 97
    Range % 92-101
    1:4 Average % 89
    Range % 84-93
    1:8 Average % 94
    Range % 90-98
  • 回收率：
    The recovery of human LMTK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 87-98
    EDTA plasma (n=4) 105 100-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1200 2.687 2.532 2.610 2.450
    600 1.994 2.018 2.006 1.846
    300 1.269 1.295 1.282 1.122
    150 0.716 0.728 0.722 0.562
    75 0.475 0.483 0.479 0.319
    37.5 0.321 0.307 0.314 0.154
    18.75 0.243 0.239 0.241 0.081
    0 0.158 0.162 0.160 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription. Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons.
  • 亚细胞定位：
    Membrane; Single-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Golgi apparatus membrane. Note=Punctate pattern in cell projections.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family
  • 数据库链接：

    HGNC: 19295

    UniGene: Hs.207426