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  4. Human Semenogelin-2(SEMG2) ELISA kit

Human Semenogelin-2(SEMG2) ELISA kit

  • 中文名称：
    人精囊蛋白-2(SEMG2)酶联免疫试剂盒
  • 货号：
    CSB-EL021002HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SEMG2 ELISA Kit was designed for the quantitative measurement of Human SEMG2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    SEMG2 ELISA Kit; Semenogelin-2 ELISA Kit; Semenogelin II ELISA Kit; SGII ELISA Kit
  • 缩写：
    SEMG2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SEMG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 85-94
    1:2 Average % 92
    Range % Sep-87
    1:4 Average % 92
    Range % 85-96
    1:8 Average % 89
    Range % 87-92
  • 回收率：
    The recovery of human SEMG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 86-96
    EDTA plasma (n=4) 103 98-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.423 2.579 2.501 2.393
    1000 1.954 2.054 2.004 1.896
    500 1.265 1.302 1.284 1.176
    250 0.712 0.734 0.723 0.615
    125 0.416 0.405 0.411 0.303
    62.5 0.251 0.266 0.259 0.151
    31.25 0.176 0.183 0.180 0.072
    0 0.107 0.108 0.108 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
  • 基因功能参考文献：
    1. SgII transcripts were demonstrated in several tissues, with the strongest signals coming from seminal vesicles, vas deferens, prostate, epididymis and trachea. PMID: 12200457
    2. SGII is a novel target for protein S-nitrosylation in spermatozoa. PMID: 17683036
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Semenogelin family
  • 组织特异性：
    Seminal vesicles, and to a much lesser extent, epididymis.
  • 数据库链接：

    HGNC: 10743

    OMIM: 182141

    KEGG: hsa:6407

    STRING: 9606.ENSP00000361855

    UniGene: Hs.537218