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  4. Human S100 calcium binding protein A6/calgranulin A(S100A6) ELISA Kit

Human S100 calcium binding protein A6/calgranulin A(S100A6) ELISA Kit

  • 中文名称：
    人S100钙结合蛋白A6/钙粒蛋白A(S100A6)酶联免疫试剂盒
  • 货号：
    CSB-E13089h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human S100A6 ELISA Kit was designed for the quantitative measurement of Human S100A6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.2 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/ml.

  • 别名：
    2A9 ELISA Kit; 5B10 ELISA Kit; CABP ELISA Kit; CACY ELISA Kit; Calcyclin ELISA Kit; Growth factor inducible protein 2A9 ELISA Kit; Growth factor-inducible protein 2A9 ELISA Kit; MLN 4 ELISA Kit; MLN4 ELISA Kit; OTTHUMP00000015472 ELISA Kit; OTTHUMP00000015473 ELISA Kit; PRA ELISA Kit; PRAGrowth factor inducible protein 2A9 ELISA Kit; Prolactin receptor associated protein ELISA Kit; Prolactin receptor-associated protein ELISA Kit; Protein S100 A6 ELISA Kit; Protein S100-A6 ELISA Kit; S100 A6 ELISA Kit; S100 calcium binding protein A6 (calcyclin) ELISA Kit; S100 calcium binding protein A6 ELISA Kit; S100 calcium-binding protein A6 ELISA Kit; S100A6 ELISA Kit; S10A6_HUMAN ELISA Kit
  • 缩写：
    S100A6
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/ml-2000 pg/ml
  • 灵敏度：
    7.81 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:20 Average % 95    
    Range % 87-99    
    1:40 Average % 101    
    Range % 94-105    
    1:80 Average % 97    
    Range % 87-101    
    1:160 Average % 105    
    Range % 95-109    
  • 回收率：
    The recovery of human S100A6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 102 93-106    
    EDTA plasma (n=4) 100 91-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.635 2.578 2.607 2.512    
    1000 2.036 2.193 2.115 2.020    
    500 1.663 1.612 1.638 1.543    
    250 1.190 1.165 1.178 1.083    
    125 0.675 0.686 0.681 0.586    
    62.5 0.385 0.389 0.387 0.292    
    31.2 0.233 0.237 0.235 0.140    
    0 0.093 0.097 0.095 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
  • 基因功能参考文献：
    1. aberrant S100A16 expression might be modulated by its DNA hypomethylation and serves as an independent prognostic indicator of unfavorable overall survival and recurrence-free survival in non-small cell lung adenocarcinoma PMID: 29746588
    2. High expression of S100A6 in ICC defines a special group of patients with worse clinical characteristics and outcomes. Besides, S100A6 may promote ICC proliferation via activating p38/MAPK pathway. PMID: 28984474
    3. The results suggest that binding of S100A6 to integrin beta1 affects cell adhesion/proliferation due to activation of ILK and FAK signaling pathways. PMID: 29020611
    4. s demonstrated that the expression of S100A6 was significantly associated with patient age and tumor differentiation. PMID: 29053662
    5. Data show that S100 calcium binding protein A6 (S100A6) is required for the Ca2+-dependent nuclear translocation of calcyclin binding protein (CacyBP/SIP) in colon cancer SW480 cells. PMID: 29534068
    6. Isothermal titration calorimetry measured the binding of peptides with diverse sequence and biochemistry to human S100A5 and S110A6. These proteins bound distinct, but overlapping, sets of peptide targets. The specificity of S100 peptide interfaces is likely important for the biology of these proteins. PMID: 29240404
    7. S100A6 is a potential risk marker for screening of cholangiocarcinoma. PMID: 29629840
    8. The S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca(2+)-dependent regulation of centrosomal function. PMID: 28765046
    9. REVIEW: summary of novel discoveries concerning S100A6 targets, its involvement in cellular signaling pathways, and presence in stem/progenitor cells, extracellular matrix and body fluids of diseased patients PMID: 28343163
    10. Results showed that S100A6 was markedly up-regulated in nasopharyngeal carcinoma (NPC) tissues and cell lines and, may promote NPC development via the activation of p38/MAPK signaling pathways. PMID: 27596819
    11. As an intracellular protein, S100A6 has been implicated in the regulation of several cellular functions, such as proliferation, apoptosis, the cytoskeleton dynamics, and the cellular response to different stress factors. S100A6 can be secreted/released by certain cell types which points to extracellular effects of the protein. [review] PMID: 28417162
    12. suppression of S100A6 expression with RNAi significantly decreased the expression levels of beta-catenin, inhibited the growth and migration of eutopic endometrial stromal cells, and promoted their apoptosis. PMID: 28075439
    13. Study reports the crystallographic structure of an S100-bound full-length RAGE ectodomain. The structure reveals a unique dimeric conformation of RAGE, which appears suited for signal transduction, and shows that the S100A6 protein adopts a non-canonical homodimeric arrangement. PMID: 27818100
    14. STAT1 suppression by S100A6 may represent a promising therapeutic target to facilitate reendothelialization in damaged vessels. PMID: 27386938
    15. findings strongly suggest that S100A6 may promote OS cell proliferation and OS tumor growth at least in part by facilitating cell cycle progression, preventing apoptosis, and inhibiting osteogenic differentiation PMID: 26646427
    16. S100A6 induces EMT and promotes cell migration and invasion in a beta-catenin-dependent manner. PMID: 25799022
    17. Weak or absent S100A6 staining supports a diagnosis of pilar leiomyoma, whereas strong positive staining supports a diagnosis of cutaneous leiomyosarcoma. PMID: 26238340
    18. Elevated S100A6 enhances tumorigenesis and suppresses CXCL14-induced apoptosis in clear cell renal cell carcinoma. PMID: 25760073
    19. Applying this serum marker to clinical practice would require less-invasive examinations of patients and would help to detect life-threatening cancerous lesions earlier than current modalities. PMID: 25743341
    20. Results obtained proved that increased S100A6 content in keratinocytes dramatically changed the pace and extent of epidermal differentiation PMID: 25450463
    21. High-level S100A6 promotes metastasis and predicts the outcome of T1-T2 stage in clear cell renal cell carcinoma PMID: 25120023
    22. Data indicate that the interaction between S100A6 and integrin beta1, was confirmed by ELISA. PMID: 25256682
    23. In Wharton's jelly of preeclamptic tissue S100 calcium binding protein A6(S100A6) is up-regulated and binds to different targets than in control suggesting involvement in development of preeclampsia PMID: 24746261
    24. Following myocardial infarction, S100A6 expression levels increased in cardiomyocytes. PMID: 23844739
    25. S100A6 may be involved in promotion and progression of human liver cancer. PMID: 24281831
    26. Expression of S100A6 and MMP9 in combination is associated with the development of squamous cell carcinoma. PMID: 23993025
    27. S100A6 and the TAZ2 domain of p300 bind p53 with similar affinities and S100A6 effectively competes with TAZ2 for binding to p53. PMID: 23796514
    28. The S100A6 mutant protein interacts with the RAGE V domain in a symmetrical fashion forming a heterotetrameric complex. PMID: 23537648
    29. native S1006 seeds SOD1 aggregation, shortening its nucleation process. This suggests a cross-talk between these two proteins involved in ALS. PMID: 23076148
    30. Results show strong correlations between mRNA levels of PRA and PRB, protein levels of hormone receptors, HER/ErbB receptors and ligands network, and suggest that crosstalks between PR and the HER family are a hallmark of breast cancer growth. PMID: 22505232
    31. Depletion of endothelial S100A6 levels also elevated beta-galactosidase expression, which is an important hallmark of cellular senescence and exit from the mammalian cell cycle PMID: 23095053
    32. Upregulated expression of S100A6 is associated with gastric cancer. PMID: 22681645
    33. Introduced a simple and efficient method for producing high-purity recombinant human S100A6 from Escherichia coli culture with low level of endotoxin. We further demonstrated its biological activities for triggering SH-SY5Y cells apoptosis in vitro. PMID: 22450162
    34. S100A6 interacts with lamin A/C, a protein known to be implicated in colon carcinogenesis. PMID: 22560296
    35. the overexpression of thioredoxin,S100-A10 and S100-A6 specifically distinguished metastatic from non-metastatic tumors PMID: 21938494
    36. new insight into the interaction between S100 proteins and CacyBP/SIP PMID: 22295074
    37. Calcyclin was identified to be underexpressed in small cell lung cancers, as compared with non-small cell lung cancers and normal lung tissue. PMID: 22192801
    38. results show the presence of S100A6 in umbilical cord and suggest the involvement of this protein in intra- and extra-cellular signalling pathways in this tissue PMID: 21923636
    39. S100A6 is necessary for nuclear translocation of the Sgt1 protein PMID: 20213445
    40. Serum levels of S100B, S100A6 and S100P are associated with acute coronary syndrome, and serum levels and myocardial expression of these proteins are related to infarct size. PMID: 21663912
    41. In melanocytic neoplasms composed of small spindle cells, patchy S100A6 staining should not be interpreted as evidence of supporting a diagnosis of melanoma PMID: 21039744
    42. Results suggested that S100A6 plays an important role in the progression of gastric cancer, affecting patient prognosis, and is up-regulated by epigenetic regulation. PMID: 20581057
    43. This study supports a role of S100A6 in thyroid tumorigenesis and as a potential aid in the discrimination between follicular thyroid tumors and papillary thyroid carcinoma. PMID: 20629554
    44. S100A6 expression decreases along with cancerization in oral squamous cell carcinoma. PMID: 20596636
    45. S100A6 expression level in cancer and metastatic lymph node was significantly higher than their matched non-neoplastic mucosa. S100A6 overexpression was associated with larger tumor size and deeper invasion. PMID: 19062716
    46. S100A6 concentration predicts peritoneal tumor burden in mice with epithelial ovarian cancer and is associated with advanced stage in patients PMID: 19888321
    47. Proteomic profiling in distinct cellular compartments led to the identification of a novel p53-dependent biomarker of telomere dysfunction, S100A6. PMID: 19834903
    48. findings suggest that up-regulation of FRalpha gene and calcyclin gene expressions induced by Allitridi may play an important role in human gastric cancer cell differentiation PMID: 11925593
    49. Results are consistent with S100A6, and most likely other S100 proteins, functioning as Ca(2+) sensors in a way analogous to the prototypical sensors calmodulin and troponin C. PMID: 11937060
    50. Sgt1 binds to S100A6 in a calcium-regulated manner PMID: 12746458

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  • 亚细胞定位：
    Nucleus envelope. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    S-100 family
  • 数据库链接：

    HGNC: 10496

    OMIM: 114110

    KEGG: hsa:6277

    STRING: 9606.ENSP00000357708

    UniGene: Hs.275243