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  4. Human Resistin ELISA KIT

Human Resistin ELISA KIT

  • 中文名称：
    人抵抗素(Resistin)酶联免疫试剂盒
  • 货号：
    CSB-E06884h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        RETN（全称Retinol-binding protein 4）是一种由脂肪细胞分泌的蛋白质，主要参与能量代谢和炎症反应等生理过程。其含量水平与肥胖、糖尿病、代谢综合征等疾病相关，研究表明RETN可促进胰岛素抵抗和炎症反应，从而导致多种代谢异常。因此，RETN可能成为这些疾病的治疗和预防的潜在靶点。
        j9九游会登录入口首页生物所提供的Human Resistin ELISA KIT属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的RETN，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    Adipose tissue specific secretory factor ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 ELISA Kit; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein ELISA Kit; CG5403 ELISA Kit; Cysteine rich secreted protein A12 alpha like 2 ELISA Kit; Cysteine rich secreted protein FIZZ3 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 2 ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit; dri ELISA Kit; FIZZ 3 ELISA Kit; FIZZ3 ELISA Kit; Found in inflammatory zone 3 ELISA Kit; HXCP 1 ELISA Kit; HXCP1 ELISA Kit; MGC126603 ELISA Kit; MGC126609 ELISA Kit; PRO1199 ELISA Kit; Resistin ELISA Kit; Resistin delta2 ELISA Kit; RETN 1 ELISA Kit; RETN ELISA Kit; RETN_HUMAN ELISA Kit; RETN1 ELISA Kit; RSTN ELISA Kit; UNQ407 ELISA Kit; XCP 1 ELISA Kit; XCP1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-103  
    1:2 Average % 94  
    Range % 91-98  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 86  
    Range % 80-92  
  • 回收率：
    The recovery of human resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 90-104  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.285 2.330 2.308 2.204  
    10 1.350 1.427 1.389 1.285  
    5 0.701 0.733 0.717 0.613  
    2.5 0.400 0.424 0.412 0.308  
    1.25 0.256 0.268 0.262 0.158  
    0.625 0.178 0.169 0.174 0.070  
    0.312 0.132 0.129 0.131 0.027  
    0 0.105 0.102 0.104 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    RETN是一个脂肪细胞因子，也被称为“Resistin”，可以影响体内脂肪代谢和胰岛素敏感性。最近的研究表明，RETN可能参与肥胖、糖尿病、心血管疾病、炎症等疾病的发生和发展，成为一个重要的生物标志物。此外，一些研究还发现RETN在癌症的发生和进展中也可能扮演一定的角色。
  • 功能：
    Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Promotes chemotaxis in myeloid cells.
  • 基因功能参考文献：
    1. The results of this study indicated that resistin gene polymorphisms might affect the genetic predisposition of rheumatoid arthritis in Chinese population. PMID: 29978502
    2. It was demonstrated that high resistin expression was predominantly observed in lung adenocarcinoma tissues. It is associated with a more malignant clinicopathological status and poorer survival. Resistin could promote A549 and H1975 tumor cell proliferation, migration and invasion while inhibit their apoptosis in vitro. PMID: 30272365
    3. A multi-cytokine resistin pathway is operational in humans, and it plays a substantial role in cardiovascular events in high-risk individuals. PMID: 28290549
    4. An increase in resistin concentration expressed also as resistin/BMI, and resistin/adiponectin ratios, observed in children with cystic fibrosis may suggests that this adipokine is involved in the inflammatory process underlying the disease and is related to worse spirometric parameters describing airways obstruction. PMID: 29465158
    5. n T2 mice, the mRNA expression of Retn showed a moderate up-regulation (fold change=8.32; p=0.0019) in the adipose tissues. Iapp expression was also significantly up-regulated (fold change=9.78; p=0.012). Moreover, a 6.36-fold up-regulation for Drd5 was observed in the adipose tissues of T2 mice PMID: 29936463
    6. RETN and CAP1 polymorphisms and gene expression may be potential biomarkers for breast cancer risk PMID: 29641286
    7. resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID: 29604272
    8. Study provides clear evidence that resistin is a clinically relevant endogenous ligand for TLR4, which promotes tumor progression via TLR4/NF-kappaB/STAT3 signaling. PMID: 28991224
    9. women with gestational diabetes showed significantly low adiponectin and high resistin levels when compared with control group. PMID: 29207892
    10. increased levels of resistin in erosive and non-erosive hand osteoarthritis PMID: 29105498
    11. Resistin promotes a shift from proliferation to apoptosis in vascular smooth muscle cells and macrophage co-culture systems with cellular composition similar to that found in vulnerable regions of plaques. PMID: 29361366
    12. The inverse association between serum resistin and n-3 PUFA intake was strongest in SNP-420 G/G genotype in the Japanese cohort. PMID: 29044636
    13. Our results do not support a relationship among resistin levels, obesity and insulin resistance in humans PMID: 29280648
    14. rs1862513 and rs3745368 in resistin genes are not associated with the presence of MetS in a Han Chinese population. PMID: 29386698
    15. For T2D, when the subjects were separated by resistin into tertiles, elevated resistin was associated with a benefit for the Castelli 1 index PMID: 28760596
    16. The -420C>G resistin gene polymorphism appears to be associated with more severe stroke and higher in-hospital mortality in patients with acute ischemic stroke. Higher leptin levels appear to be related to favorable functional outcome. PMID: 29217361
    17. Resistin contributes to the pathogenesis of rheumatoid arthritis by increasing chemokine production by fibroblast-like synoviocytes PMID: 29191223
    18. Studies identify a critical role for hRetn in blocking lipopolysaccharide function with important clinical significance in helminth-induced immunomodulation and sepsis. PMID: 29133417
    19. Plasma resistin did not show any association with the degree of vascular damage in non-hypersomnolent obstructive sleep apnoea patients. PMID: 27256793
    20. In anorexia nervosa subjects, GG genotype of RETN c.-180 was significantly more frequent than in control group. PMID: 28759190
    21. serum and follicular fluid resistin levels were significantly higher in the endometriosis group PMID: 29442466
    22. Our data suggest that adiponectin and resistin are altered in non-obese chronic migraineurs. PMID: 27553954
    23. Studied the association of resistin (RETN); single nucleotide polymorphisms (SNPs) and serum RETN levels in obese and non-obese Tunisians. PMID: 28393393
    24. This study has shown Resistin, and Visfatin synergistically increased gastric cancer cell proliferation and enhanced the telomerase gene expression, showing that these two hormones in gastric cancer tissue could cooperatively accelerate cancer cell growth PMID: 29228527
    25. resistin is differentially expressed in endometrial tissues from women with endometriosis and imply a role for resistin in endometriosis-associated pelvic inflammation PMID: 28681517
    26. Resistin, but not copeptin levels are higher in acute ischemic stroke patients early after the stroke onset, than in age and gender matched stroke-free controls. Moreover, higher copeptin concentrations are predictive of poor short term functional outcome after ischemic stroke PMID: 28845746
    27. Significant up-regulation of PBMCs CAP1, CD36 mRNA and plasma resistin found in significant coronary artery disease, as well as in nonsignificant coronary artery disease compared to control group, indicates that resistin could be able to exert its effects stronger on cells with up-regulated CAP1 mRNA thus contributing atherosclerosis development. PMID: 28707728
    28. Results demonstrate an association between RETN gene variants and risk of rheumatoid arthritis disease. RETN SNPs were significantly associated with clinical therapies and C-reactive protein serum marker of rheumatoid arthritis in the Chinese Han population. PMID: 29561430
    29. Studied adiponectin (ADIPOQ), leptin (LEP), leptin receptor (LEPR), and resistin (RETN) single nucleotide polymorphisms and their association in obesity. Results showed that ADIPOQ 4522CPMID: 28195351
    30. Results show that adiponectin, resistin and visfatin are present in osteophytes but have no direct influence on osteophyte development. Although these adipokines induce several inflammatory mediators, they do not affect osteoblast- or chondrocyte-related mechanisms of osteophyte development. PMID: 27884778
    31. Fine-tuned by RETN SNPs, intrahepatic, multi-cellular resistin reinforced IFNL3 in eliminating HCV via immunomodulation to counteract pro-inflammation. PMID: 27477870
    32. Dementia of vascular origin is characterized by elevated resistin levels. PMID: 28421328
    33. Resistin probably plays a role in the pathogenesis of hepatic insulin resistance and aggravates pathologic changes in the liver of patients with non-alcoholic fatty liver disease PMID: 29363914
    34. Resistin and NGAL correlate with expression of endothelial cell adhesion molecules in sepsis. PMID: 28424824
    35. the role of resistin (an adipokine) in the development of gestational diabetes mellitus PMID: 28685604
    36. high resistin levels are associated with increased obesity-related cancer risk [meta-analysis] PMID: 27509174
    37. Results showed that the RETN SNP rs3219175 with AG or at least 1 A allele was associated with a higher risk of lung cancer than wild-type (GG) carriers. Moreover, the RETN SNP rs3219175 with AG or AG + AA alleles was associated with a higher risk of distant metastasis than that in patients carrying GG alleles. PMID: 29384942
    38. Report decreased serum resistin level in Grave's disease patients. Suggest that serum resistin is primarily secreted from circulating neutrophils and down-regulated by excessive thyroid hormones in GD patients. PMID: 27637079
    39. Change in salivary resistin level was not significantly associated with periodontal outcomes. In obese Malaysians, NSPT significantly improved PS and GBI, and improved PPD and CAL for shallow and moderately deep pockets but not for deep pockets PMID: 28049964
    40. Therefore, rs1423096 and rs10401670, in addition to SNP-420 and SNP-358, were identified as possible functional variants affecting circulating resistin by the genome-wide search in the Japanese population. PMID: 27664181
    41. In this meta-analysis, serum resistin levels are associated with and increased risk of acute cerebral infarction in Asians, but not in Caucasians. PMID: 26899574
    42. the mutant genotype of two SNPs of the RETN gene (rs1862513 and rs10401670) was associated with a lack of change in resistin secondary to biliopancreatic diversion PMID: 27118275
    43. Serum resistin level was higher in CKD ( Chronic Kidney Diseases ) patients compared to control subjects. PMID: 25385911
    44. The present study suggests that the G/G genotype of RETN rs1862513 could be a predictor of the reduction of HOMA-IR, insulin, fasting glucose and LDL cholesterol secondary to a hypocaloric diet in obese subjects. PMID: 28064279
    45. The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
    46. Results provide evidence that RETN SNPs were functional and associated with the levels of serum creatinine and cholesterol. PMID: 29101068
    47. Resistin-associated VSMC dysfunction and intimal hyperplasia are related to PKCepsilon-dependent Nox activation and ROS generation PMID: 27573736
    48. Adiponectin and resistin show an additive independent effect on all-cause mortality in patients with type 2 diabetes PMID: 27175608
    49. Amendment of the cytokine profile in macrophages subsequent to their interaction with smooth muscle cells: Differential modulation by fractalkine and resistin. PMID: 27180200
    50. The strongest OR was for CXCL8 (interleukin-8) in serum (96.8, 95% CI: 11.9-790.2). Of these 15 markers, 6 were also significantly elevated in serum from Chile (CCL20, C-reactive protein, CXCL8, CXCL10, resistin, serum amyloid A). PMID: 27173614

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Resistin/FIZZ family
  • 组织特异性：
    Expressed in white adipose tissue (at protein level). Widely expressed, with particularly strong expression in lung, bone marrow, breast and peripheral blood. Expressed strongly in bone marrow and at lower levels in lung, but not detected in other tissues
  • 数据库链接：

    HGNC: 20389

    OMIM: 605565

    KEGG: hsa:56729

    STRING: 9606.ENSP00000221515

    UniGene: Hs.283091