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  4. Human Repetin(RPTN) ELISA kit

Human Repetin(RPTN) ELISA kit

  • 中文名称：
    人Repetin蛋白(RPTN)酶联免疫试剂盒
  • 货号：
    CSB-EL020501HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human RPTN ELISA Kit was designed for the quantitative measurement of Human RPTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

  • 别名：
    RPTN ELISA Kit; Repetin ELISA Kit
  • 缩写：
    RPTN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human RPTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %88-97
    1:2Average %86
    Range %80-92
    1:4Average %96
    Range %87-102
    1:8Average %93
    Range %89-97
  • 回收率：
    The recovery of human RPTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9388-98
    EDTA plasma (n=4)9087-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.796 2.869 2.833 2.711
    202.131 1.994 2.063 1.941
    101.152 1.243 1.198 1.076
    50.705 0.732 0.719 0.597
    2.50.343 0.366 0.355 0.233
    1.250.236 0.224 0.230 0.108
    0.6250.218 0.210 0.214 0.092
    00.124 0.120 0.122 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.
  • 基因功能参考文献：
    1. The single-nucleotide polymorphisms of CRNN (rs941934) and RPTN (rs3001978, rs28441202) may contribute to Atopic Dermatitis development, but further studies on a larger group of Atopic Dermatitis patients are needed to verify this assumption. PMID: 28219068
    2. RPTN plays a potential role in emotional and cognitive processing; its decrease in serum may indicate its involvement in the pathogenesis of schizophrenia and bipolar disorder PMID: 25613293
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    S100-fused protein family
  • 组织特异性：
    Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheath and in the filiform papilli of the tongue.
  • 数据库链接：

    HGNC: 26809

    OMIM: 613259

    KEGG: hsa:126638

    STRING: 9606.ENSP00000317895

    UniGene: Hs.376144