Human Proto-oncogene serine/threonine-protein kinase pim-1(PIM1) ELISA kit

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells. Promotes brown adipocyte differentiation.

1. CD95-mediated apoptosis induces Pim-1 down-regulation in Burkitt's lymphoma (BL) B-cells, but Pim-1 down-regulation cannot fully eradicate BL and leukaemia. PMID: 27641442
2. Data show that pim-1 oncogene protein (PIM1) expression was elevated in T-cell lymphomas (TCLs) cells. PMID: 30020405
3. Results provide evidence that glucose deprivation is one of the mechanisms that leads to elevated Pim1 expression in colorectal cancer (CRC), and Pim1 upregulation ensures CRC growth in response to glucose deprivation by facilitating the Warburg effect in a compensatory way. PMID: 29516572
4. data suggest that PIM1/2 kinase overexpression is a common feature of male reproductive organs tumors, which provoke tissue alterations and a large inflammatory response that may act synergistically during the process of tumorigenesis. PMID: 27901106
5. PIM-1 mRNA levels may be an independent prognostic factor in acute myeloid leukemia. PMID: 28851457
6. PIM1 role in cell proliferation, migration and apoptosis in triple-negative breast cancer [review] PMID: 28721678
7. may contribute to placental inflammation in pregnancies complicated by maternal obesity PMID: 28487013
8. Inhibition of PIM1 kinase attenuates inflammation-induced pro-labor mediators in human fetal membranes in vitro. PMID: 28333279
9. PIM1 destabilization is associated with cancer. PMID: 26993775
10. The Ser/Thr-protein kinase-1 (PIM-1) was identified as a direct target of miR-328. PMID: 27448984
11. Data show that PIM1 contributes to melanoma cell proliferation and tumor growth in vivo; however, the presence of PIM2 and PIM3 could also influence the outcome. PMID: 27448973
12. High expression level of PIM is associated with neoplasms. PMID: 26956053
13. This review summarizes effects of PIM kinases and their substrates especially on cancer cell migration, invasion and metastatic growth, based on data from cell-based assays, animal experiments and patients. PMID: 29108877
14. Results show that PIM-1 is upregulated in pancreatic cancer tissues and plasma. Its knockdown in pancreatic cancer cells suppressed proliferation, induced cell cycle arrest, enhanced apoptosis, resensitized cells to gemcitabine and erlotinib treatment, and inhibited ABCG2 and EZH2 mRNA expression. PMID: 27596051
15. Results show that PIM1 is overexpressed in breast cancer tumors and provide evidence for its role in tumor resistance to PI3K inhibitors. PMID: 27604488
16. These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines which may indicate similar functions in primary cells. PMID: 28729093
17. Down-regulation of UHRF1 is an important mechanism of PIM1-mediated cellular senescence. PMID: 28394343
18. PIM kinases in classical Hodgkin lymphoma exhibit pleiotropic effects, orchestrating tumor immune escape and supporting Reed-Sternberg cell survival. PMID: 28698206
19. critical for the growth and metastasis of osteosarcoma cells PMID: 26687194
20. Triple negative breast cancer cells, but not nonmalignant mammary epithelial cells, were dependent on PIM1 for proliferation and protection from apoptosis. PMID: 27775704
21. PIM1 expression was higher in triple negative breast tumors than in estrogen and progesterone receptor positive tumors. PMID: 27775705
22. High PIM1 expression is associated with osteosarcoma. PMID: 27826617
23. downregulation of PIM1 led to suppression of cell proliferation by cell cycle arrest at G1 phase and suppression of cell invasion and migration. PMID: 28197633
24. Pim-1L protects hepatic ABCA1 from lysosomal degradation by facilitating the physical interaction between ABCA1 and liver X receptor beta and subsequent stabilization of the ABCA1-Pim-1L complex and thereby regulates the circulating level of high-density lipoprotein. PMID: 27765770
25. Furthermore, the Pim-1-HBP1 positive feedback loop exerts its effect by regulating the senescence markers DNMT1 and p16 and the apoptosis marker Bax. The Pim-1-HBP1 axis thus constitutes a novel checkpoint pathway critical for the inhibition of tumorigenesis. PMID: 28348080
26. Overexpression of PIM1 partially rescued miR-542-3p-mediated suppression of cell migration, invasion and EMT. Our results collectively indicate that miR-542-3p serves as a metastasis suppressor in melanoma, supporting its utility as a promising therapeutic candidate. PMID: 27107696
27. Data show that cytoplasmic irradiation mediate expression level of Pim-1, which lead to glycolytic shift in SAE cells. PMID: 28170315
28. Pim1 role in the apoptosis and cell proliferation of human esophageal cancer cells PMID: 27983525
29. findings aid in understanding the tumor-suppressive role of miR-124-3p in astrocytoma pathogenesis through the inhibition of PIM1 translation PMID: 27088547
30. hypoxia induced miR-124 and miR-144 downregulation may contribute to a prosurvival mechanism of prostate cancer cells to hypoxia and irradiation at least through attenuated suppressing of PIM1. PMID: 26990493
31. Results show loss of miR-1 and miR-214 expression and high expression of their target gene, PIM1, in malignant mesothelioma suggesting a role in carcinogenesis of mesothelioma. PMID: 26820394
32. we examine the therapeutic implications of Pim1 to encourage the personalization of cardiac regenerative therapy PMID: 26563999
33. Data suggest that combining PIM and JAK2 kinase inhibitors may offer a more efficacious therapeutic approach for myeloproliferative neoplasms (MPNs) over JAK2 inhibitor mono-therapy. PMID: 26472029
34. A high percentage of urothelial carcinomas express Pim kinases. Pim expression differs in NILG, NIHG, and IHG lesions. PMID: 26551340
35. Downregulation of microRNA33a promotes the expression CDK6, CCND1, and PIM1, and gastric cancer cell proliferation. PMID: 26352175
36. glycogen synthase kinase 3 beta (GSK3B) and the forkhead box P3 (FOXP3) transcription factors are direct PIM1 targets. PMID: 26934497
37. By associating with PIM-1L, CD180 can thus obtain autonomous signaling capabilities, and this complex is then channeling inflammatory signals into B cell survival programs PMID: 26555723
38. Pim1 kinase activity maintains airway epithelial integrity and protects against house dust mite-induced proinflammatory activation of the airway epithelium. PMID: 26453516
39. Pim-1 and Pim-3 enhance phosphorylation and cell surface expression of CXCR4 in prostate cancer cells. PC-3 prostate cancer cells overexpressing either Pim-1 or Pim-3 kinases form larger xenograft tumors than the parental PC-3 cells. PMID: 26075720
40. PIM1 is up-regulated by hypoxia in hepatocellular carcinoma and promotes tumor growth and metastasis by facilitating cancer cell glycolysis. PMID: 25834102
41. This study demonstrates the oncogenic role of Pim-1 in ACC. The findings also suggest that Pim-1 may serve as a neoteric therapeutic target and potential prognostic marker for ACC cancer PMID: 25551195
42. We now demonstrate a molecular mechanism which reveals a direct role for EBNA3C in enhancing Pim-1 expression in EBV-infected primary B-cells. PMID: 25121590
43. Pim kinase may represent a new host factor for HCV entry. Pim1 is an oncogenic serine/threonine kinase. HCV NS5A protein physically interacts with Pim1 and contributes to Pim1 protein stability. PMID: 26202252
44. PIM1 overexpression is associated with prostate cancer. PMID: 24771642
45. Molecular dynamics studies showed that only GTP decreases the formation of the GBP1:PIM1 complex through an allosteric mechanism, outlining the rational for the identification of new compounds potentially able to revert resistance to paclitaxel. PMID: 25081641
46. important role in progression of pre-malignant high grade prostatic intra-epithelial neoplasia to malignant prostatic carcinomas [review] PMID: 25553374
47. In comparison with normal brain, a strong upregulation of Pim1 was demonstrated in human GBM samples. Notably, patients with short overall survival showed a significantly higher Pim1 expression compared with GBM patients who lived longer than the median. PMID: 25155357
48. Pim1 function depends upon intracellular localization in human cardiac progenitor cells PMID: 25882843
49. These results point on PIM1 as a novel factor in regulation of the phenotype and differentiation of fibroblasts in prostate cancer. PMID: 25451079
50. Data indicate that serine/threonine-protein kinase PIM1 expression was noted in each case of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). PMID: 24547709

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[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Cell membrane.

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PIM subfamily

Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.

HGNC: 8986

OMIM: 164960

KEGG: hsa:5292

STRING: 9606.ENSP00000362608

UniGene: Hs.81170