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  4. Human Protein prune homolog(PRUNE) ELISA kit

Human Protein prune homolog(PRUNE) ELISA kit

  • 中文名称：
    人蛋白prune同源物(PRUNE)酶联免疫试剂盒
  • 货号：
    CSB-EL018831HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PRUNE ELISA Kit was designed for the quantitative measurement of Human PRUNE protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

  • 别名：
    DRES-17 ELISA Kit; DRES17 ELISA Kit; Drosophila-related expressed sequence 17 ELISA Kit; hPrune ELISA Kit; HTcD37 ELISA Kit; Protein prune homolog ELISA Kit; PRUNE ELISA Kit; Prune homolog ELISA Kit; PRUNE like protein ELISA Kit; PRUNE_HUMAN ELISA Kit; TcD37 homolog ELISA Kit
  • 缩写：
    PRUNE
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    47 pg/mL-3000 pg/mL
  • 灵敏度：
    11.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRUNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %83-94
    1:2Average %96
    Range %91-104
    1:4Average %89
    Range %93-96
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of human PRUNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-96
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.283 2.183 2.233 2.111
    15001.647 1.567 1.607 1.485
    7500.982 0.952 0.967 0.845
    3750.532 0.512 0.522 0.400
    187.50.384 0.374 0.379 0.257
    940.247 0.237 0.242 0.120
    470.199 0.189 0.194 0.072
    00.122 0.121 0.122 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.
  • 基因功能参考文献：
    1. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. PMID: 28334956
    2. These results further delineate a new PRUNE1-related syndrome, and highlight the importance of periodic data re-annotation in individuals who remain without a diagnosis after undergoing genome-wide testing. PMID: 28211990
    3. h-Prune expression is necessary for cancer cell motility and EMT and is associated with liver and lung metastasis in colorectal cancer cells. h-Prune could be a new prognostic marker and molecular target for CRLM. PMID: 27037526
    4. Together these studies define PRUNE as a molecule fundamental for normal human cortical development and define cellular and clinical consequences associated with PRUNE mutation PMID: 28334956
    5. h-prune is frequently expressed in anaplastic thyroid cancer cells and lymph nodes metastasis, and promotes migration and invasion of anaplastic thyroid cancer cells and metastasis in an anaplastic thyroid cancer model. PMID: 27109060
    6. s assessed h-Prune levels in peripheral blood of lung cancer patients using ELISA assay, showing that h-Prune is an early diagnostic marker for lung cancer. PMID: 25026278
    7. Data indicate that the D388A and D422A mutant h-Prune proteins interact weakly with Nm23-H1. PMID: 23448979
    8. Increased amplification of PRUNE is associated with T4 breast carcinoma. PMID: 20735841
    9. prune has a role in breast neoplasm aggressiveness PMID: 15671547
    10. GSK-3 and h-prune cooperatively regulate the disassembly of focal adhesions to promote cell migration and that h-prune is useful as a marker for tumor aggressiveness. PMID: 16428445
    11. Prune is composed of two independent active sites and two interaction sites for the assembly of oligomeric signalling complexes PMID: 17655525

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  • 相关疾病：
    Neurodevelopmental disorder with microcephaly, hypotonia, and variable brain anomalies (NMIHBA)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed.
  • 蛋白家族：
    PPase class C family, Prune subfamily
  • 组织特异性：
    Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
  • 数据库链接：

    HGNC: 13420

    OMIM: 617413

    KEGG: hsa:58497

    STRING: 9606.ENSP00000271620

    UniGene: Hs.78524