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  4. Human Protein S100-A7A(S100A7A) ELISA kit

Human Protein S100-A7A(S100A7A) ELISA kit

  • 中文名称：
    人S100-A7A蛋白(S100A7A)酶联免疫试剂盒
  • 货号：
    CSB-EL020636HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human S100A7A ELISA Kit was designed for the quantitative measurement of Human S100A7A protein in serum, plasma, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    S100A7A ELISA Kit; S100A15 ELISA Kit; S100A7L1 ELISA Kit; Protein S100-A7A ELISA Kit; S100 calcium-binding protein A15 ELISA Kit; S100 calcium-binding protein A7-like 1 ELISA Kit; S100 calcium-binding protein A7A ELISA Kit
  • 缩写：
    S100A7A
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, urine
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 92
    Range % 89-96
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 91
    Range % 82-97
  • 回收率：
    The recovery of human S100A7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 93-101
    EDTA plasma (n=4) 86 81-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.834 2.912 2.873 2.758
    10 2.175 2.017 2.096 1.981
    5 1.286 1.218 1.252 1.137
    2.5 0.654 0.664 0.659 0.544
    1.25 0.397 0.386 0.392 0.277
    0.625 0.249 0.242 0.246 0.131
    0.312 0.167 0.162 0.165 0.050
    0 0.115 0.114 0.115 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases.
  • 基因功能参考文献：
    1. S100A15 retains the ability to coordinate zinc through incorporation of an exogenous ligand resulting in a unique zinc-binding site among S100 proteins. PMID: 22747601
    2. Molecular cloning and characterization of alternatively spliced S100A15 isoforms from psoriatic skin. PMID: 12923069
    3. The S100A15 protein has been expressed & purified to homogeneity in 2 crystal forms. Crystals of form I are triclinic. Crystals of form II are monoclinic & have a solvent content of 51.8%, corresponding to the presence of 1 monomer in the asymmetric unit. PMID: 16682778
    4. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin PMID: 17620096
    5. E. coli modulates S100A15 and IL-8 expression of keratinocytes by recognition through TLR4. PMID: 17625598
    6. study defines RAGE (receptor for advanced glycation end products) as the hS100A7 receptor, whereas hS100A15 functions through a Gi protein-coupled receptor PMID: 18606705

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Overexpressed in psoriasis.
  • 数据库链接：

    HGNC: 21657

    OMIM: 617427

    KEGG: hsa:338324

    UniGene: Hs.442337