Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Protein FAM132B(FAM132B) ELISA kit

Human Protein FAM132B(FAM132B) ELISA kit

  • 中文名称：
    人FAM132B蛋白(FAM132B)酶联免疫试剂盒
  • 货号：
    CSB-EL008059HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FAM132B ELISA Kit was designed for the quantitative measurement of Human FAM132B protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名：
    Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; ERFE_HUMAN ELISA Kit; Erythroferrone ELISA Kit; FAM132B ELISA Kit; Myonectin ELISA Kit
  • 缩写：
    FAM132B
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %86-95
    1:2Average %95
    Range %90-101
    1:4Average %87
    Range %83-94
    1:8Average %99
    Range %94-105
  • 回收率：
    The recovery of human FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-102
    EDTA plasma (n=4)8884-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.455 2.430 2.443 2.344
    5002.201 2.250 2.226 2.127
    2501.705 1.854 1.780 1.681
    1251.110 1.202 1.156 1.057
    62.50.699 0.741 0.720 0.621
    31.20.435 0.422 0.429 0.330
    15.60.266 0.270 0.268 0.169
    00.103 0.095 0.099 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry!
CSB-EL008059HU
This kit is designed against full length. Pls refer to:https://www.uniprot.org/uniprot/Q4G0M1.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Standard is 293 cell-derived recombinant protein.
Immunogen is 293 cell-derived recombinant protein.

靶点详情

  • 功能：
    Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
  • 基因功能参考文献：
    1. From a clinical point of view, erythroferrone could become a useful biological marker of iron metabolism and a therapeutic target. [review] PMID: 28666715
    2. FAM132b expression is upregulated in Congenital Dyserythropoietic Anemia type II. PMID: 27540014
    3. In hemodialysis patients DA and CERA increased levels of ERFE that regulated hepcidin 25 and led to iron mobilization from body stores during erythropoiesis. PMID: 26978524
    4. Myonectin links skeletal muscle to lipid homeostasis in liver and adipose tissue in response to alterations in energy state, revealing a novel myonectin-mediated metabolic circuit. PMID: 22351773
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Adipolin/erythroferrone family
  • 数据库链接：

    HGNC: 26727

    OMIM: 615099

    KEGG: hsa:151176

    UniGene: Hs.24951