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  4. Human Prostaglandin D2(PGD2) ELISA kit

Human Prostaglandin D2(PGD2) ELISA kit

  • 中文名称：
    人前列腺素D2(PGD2)酶联免疫试剂盒
  • 货号：
    CSB-E13898h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PGD2 ELISA Kit was designed for the quantitative measurement of Human PGD2 protein in serum, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    N/A
  • 缩写：
    PGD2
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, cell culture supernates, tissue homogenates, urine
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-94
    1:2Average %98
    Range %91-105
    1:4Average %99
    Range %94-110
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of human PGD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.985 1.854 1.920 1.840
    7501.403 1.323 1.363 1.283
    3750.912 0.886 0.899 0.819
    187.50.545 0.525 0.535 0.455
    940.371 0.359 0.365 0.285
    470.204 0.198 0.201 0.121
    23.50.129 0.126 0.128 0.048
    00.080 0.079 0.080 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

We have previously ordered these kits from you and have been running the project since 2014. I want to keep the same assays as before but I am wondering if you can provide any quality control material? In the past we didn’t have quality control material available. I understand that the kit comes with the required standards but it would be great if a positive and negative control could be purchased to be run with the kit as well. I would purchase these separately if available.Any recommendations as controls for this kit?

A:
Thanks for your inquiry.
CSB-E13898h
The standards in the kit can be used for quantitative analysis and control, so we didn't provide extra control.
Our kit is for research use only and has been optimized. Customers only need to do blank control during the assay and additional quality control is not needed. Pls let me know if you have any further questions. Thank you.