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Human Plexin-C1(PLXNC1) ELISA kit

  • 中文名称：
    人丛状蛋白C1(PLXNC1)酶联免疫试剂盒
  • 货号：
    CSB-EL018225HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PLXNC1 ELISA Kit was designed for the quantitative measurement of Human PLXNC1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    CD232 ELISA Kit; Plexin-C1 ELISA Kit; PLXC1_HUMAN ELISA Kit; Plxnc1 ELISA Kit; Virus-encoded semaphorin protein receptor ELISA Kit
  • 缩写：
    PLXNC1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %92-98
    1:2Average %93
    Range %85-97
    1:4Average %98
    Range %94-102
    1:8Average %85
    Range %80-89
  • 回收率：
    The recovery of human PLXNC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10497-108
    EDTA plasma (n=4)8782-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.016 2.118 2.067 1.873
    101.735 1.637 1.686 1.492
    51.164 1.226 1.195 1.001
    2.50.753 0.775 0.764 0.570
    1.250.552 0.543 0.548 0.354
    0.6250.413 0.405 0.409 0.215
    0.3120.312 0.304 0.308 0.114
    00.193 0.195 0.194
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.
  • 基因功能参考文献：
    1. Results indicate that long non-coding RNA (lncRNA) CASC2 can promote PLXNC1 expression by sponging miR-181a, thereby inhibiting the proliferation and invasion of melanoma cells. PMID: 29514220
    2. Plexin C1 deficiency permits synaptotagmin 7-mediated macrophage migration and enhances mammalian lung fibrosis PMID: 27609773
    3. loss of Plexin C1 in melanoma may promote early steps in melanoma progression through suppression of migration and proliferation PMID: 23160370
    4. Study reports the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1; both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers. PMID: 20727575
    5. GTP-bound form of Rab7 promotes melanogenesis through the regulation of gp100 maturation in melanoma cells. PMID: 17671519
    6. Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression. PMID: 18987670
    7. significant loss of Plexin C1 in metastatic melanoma compared with primary melanoma, suggesting the possibility that the Plexin C1 receptor is a tumor suppressor protein for melanoma PMID: 19318806

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Plexin family
  • 组织特异性：
    Detected in heart, brain, lung, spleen and placenta.
  • 数据库链接：

    HGNC: 9106

    OMIM: 604259

    KEGG: hsa:10154

    STRING: 9606.ENSP00000258526

    UniGene: Hs.584845