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  4. Human Plexin-B1(PLXNB1) ELISA kit

Human Plexin-B1(PLXNB1) ELISA kit

  • 中文名称：
    人丛状蛋白B1(PLXNB1)酶联免疫试剂盒
  • 货号：
    CSB-EL018222HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PLXNB1 ELISA Kit was designed for the quantitative measurement of Human PLXNB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 别名：
    PLXNB1 ELISA Kit; KIAA0407 ELISA Kit; PLXN5 ELISA Kit; SEP ELISA Kit; Plexin-B1 ELISA Kit; Semaphorin receptor SEP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-96
    1:2 Average % 87
    Range % 82-91
    1:4 Average % 96
    Range % 92-100
    1:8 Average % 90
    Range % 85-95
  • 回收率：
    The recovery of human PLXNB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 96-107
    EDTA plasma (n=4) 84 80-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    5000 2.543 2.446 2.495 2.389
    2500 1.619 1.534 1.577 1.471
    1250 0.872 0.921 0.897 0.791
    625 0.508 0.520 0.514 0.408
    312 0.335 0.318 0.327 0.221
    156 0.217 0.205 0.211 0.105
    78 0.151 0.159 0.155 0.049
    0 0.104 0.107 0.106 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Receptor for SEMA4D. Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.
  • 基因功能参考文献：
    1. Analysis of the interaction of Plexin-B1 and Plexin-B2 with Rnd family proteins shows lack of binding specificity. PMID: 29040270
    2. The positive expression of both Sema4D and PlexinB1 was found to be an independent risk factor for a worse survival in colorectal cancer. PMID: 27456345
    3. Loss of plexin B1 expression might play a pivotal role in enhancing the metastatic potential of breast cancer cells. PMID: 28739743
    4. Plexin B1 expression was regulated by TMPRSS2-ERG fusion gene in prostate cancer. PMID: 28004109
    5. Plexin-B1 mediates RhoA/integrin alphavbeta3 involved in the PI3K/Akt pathway and SRPK1 to influence the growth of glioma cell, angiogenesis, and motility in vitro and in vivo. PMID: 26944058
    6. results show that Sema4D/plexin-B1 signaling promotes the translocation of androgen receptor to the nucleus and thereby enhances AR transcriptional activity PMID: 25982277
    7. Plexin-B1 induces cutaneous squamous cell carcinoma cell proliferation, migration, and invasion by interacting with Sema4D. Plexin-B1 might serve as a useful biomarker and/or as a novel therapeutic target for cSCC. PMID: 26051877
    8. Results show that decreased expression of Sema4D, plexin-B1 and -B2 was associated with local recurrence and poor prognosis of breast neoplasm. PMID: 26035216
    9. Dysregulation of the vascular endothelial growth factor and semaphorin ligand-receptor families in prostate cancer metastasis PMID: 26341082
    10. Blocking of CD100, plexin B1 and/or B2 in adhesion experiments have shown that both CD100 and plexins act as adhesion molecules involved in monocyte-endothelial cell binding. PMID: 26275342
    11. Activation of endogenous plexin-B1 enhances cell migration and tumor invasiveness in prostate cancer cells. PMID: 23775445
    12. Data indicate that Rnd1 efficiently displaces Rac1 from its complex with Plexin-B1 but not vice versa. PMID: 23603360
    13. ErbB-2 overexpression in human breast & ovarian cancer cell lines leads to phosphorylation & activation of Plexin-B1. This was required for ErbB-2-dependent activation of RhoA & RhoC & promoted invasive behavior. PMID: 22378040
    14. PlexinB1 mutations block plexinB1-mediated signalling pathways that inhibit cell motility. PMID: 22404908
    15. evidence that Sema4D/Plexin-B1-mediated NF-kappaB activation and IL-8 production is critical in the generation a pro-angiogenic phenotype in endothelial cells PMID: 22028792
    16. Two crystal structures of the human Plexin-B1 cytoplasmic region in complex with a constitutively active RhoGTPase, Rac1, are reported. PMID: 21912513
    17. The binding of Sema4D to plexinB1 induced small GTPase Ras homolog gene family, member A activation and resulted in the phosphorylation of MAPK and Akt. PMID: 21812859
    18. plexin-B1, a target of miR-214, may function as an oncogene in human cervical cancer HeLa cells PMID: 21216304
    19. Plexin B1 abrogates integrin-dependent migration and activation of pp125(FAK). PMID: 21029396
    20. Plexin-B1 expression correlates with malignant phenotypes of serous ovarian tumors, probably via phosphorylation of AKT at Ser473 PMID: 21059203
    21. crystal structures of cognate complexes of the semaphorin-binding regions of plexins B1 and A2 with semaphorin ectodomains (human PLXNB1(1-2)-SEMA4D(ecto) and murine PlxnA2(1-4)-Sema6A(ecto)), plus unliganded structures of PlxnA2(1-4) and Sema6A(ecto) PMID: 20877282
    22. Sema4D/Plexin-B1 promotes the dephosphorylation and activation of PTEN through the R-Ras GAP activity, inducing growth cone collapse. PMID: 20610402
    23. Plexin B1 may function as a tumor promoter in melanomas not driven by c-Met activation. PMID: 20164843
    24. Data show that Plex-B1 assumes a predictive value for unfavourable outcome when co-expressed with Met. PMID: 19940359
    25. The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding PMID: 11937491
    26. LARG plays a critical role in plexin-B1 signaling to stimulate Rho activation and cytoskeletal reorganization. PMID: 12196628
    27. Interaction of plexin-B1 with PDZ domain-containing Rho guanine nucleotide exchange factors PMID: 12220504
    28. Plexin-B1 is an easily accessible receptor for CD100 within the immune system. The crosstalk operated by the CD100/Plexin-B1 interaction is not malignancy related but reproduces a mechanism used by normal CD5+ B cells. PMID: 12406905
    29. cleavage by proprotein convertases is a novel regulatory step for semaphorin receptors localized at the cell surface. PMID: 12533544
    30. In some human neoplastic lines, PLXB1 is overexpressed, constitutively tyrosine phosphorylated, and associated with Scatter Factor Receptors. PMID: 15184888
    31. ErbB-2-mediated phosphorylation of plexin-B1 is critically involved in Sema4D-induced RhoA activation. PMID: 15210733
    32. demonstrate that Sema4D is angiogenic in vitro and in vivo and that this effect is mediated by its high-affinity receptor, Plexin B1, and that biologic effects elicited by Plexin B1 require coupling and activation of the Met tyrosine kinase PMID: 15632204
    33. the minimal Rac1 GTPase binding domain of plexin-B1 has a ubiquitin fold, as shown by NMR PMID: 15929008
    34. Semaphorin 4D/plexin-B1 induces endothelial cell migration through the activation of PYK2, Src, and the phosphatidylinositol 3-kinase-Akt pathway PMID: 16055703
    35. Plexin-B1 contributes to trophoblast-endometrium interactions, most likely by enhancing adhesion properties. PMID: 17383649
    36. Binding of Rac1, Rnd1, and RhoD to a novel Rho GTPase interaction motif destabilizes dimerization of the plexin-B1 effector domain PMID: 17916560
    37. 13 somatic missense mutations in the cytoplasmic domain of the Plexin-B1 gene hinder Rac & R-Ras binding & R-RasGAP activity, & increase cell motility, invasion, adhesion, & lamellipodia extension. PMID: 18024597
    38. a novel mechanism by which plexin-mediated signaling can be regulated and explains how Sema4D can exert different biological activities through the differential association of its receptor with ErbB-2 and Met. PMID: 18025083
    39. NMR solution structure of the Rho GTPase binding domain; study suggests that the oncogenic behavior of the mutants can be rationalized with reference to the structure of the RBD of plexin-B1 PMID: 18275816
    40. Plexin B1 protein is absent in more than 80% of renal cell carcinomas. when we have induced plexin B1 expression with an expression vector in the renal adenocarcinoma cell line ACHN, a marked reduction in proliferation rate was produced. PMID: 18279812
    41. Mapping of the Rac1 GTPase surface that contacts the Rho GTPase binding domain of plexin-B1 by NMR confirms the plexin domain as a GTPase effector protein and regions neighboring the GTPase switch I and II regions are also involved in the interaction. PMID: 18321527
    42. Plexin B1 expression is reduced in the group of "uncoupled" stem cell-like breast cancer tumors PMID: 18417270
    43. Plexin-B1 is a dual functional GTPase activating protein for R-Ras and M-Ras, remodelling axon and dendrite morphology, respectively. PMID: 19444311
    44. B-Raf/MKK/ERK provides a permissive environment for melanoma genesis by modulating plexin B1. PMID: 19483722
    45. show here that activation of plexin-B1 by Semaphorin 4D and its subsequent tyrosine phosphorylation creates docking sites for the SH2 domains of phospholipase Cgamma. PMID: 19805522
    46. the monomeric intracellular plexin-B1 binds R-Ras but not H-Ras. These findings suggest that the monomeric form of the intracellular region is primed for GAP activity and extend a model for plexin activation. PMID: 19843518

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.
  • 蛋白家族：
    Plexin family
  • 组织特异性：
    Highly expressed in fetal kidney, and at slightly lower levels in fetal brain, lung and liver.
  • 数据库链接：

    HGNC: 9103

    OMIM: 601053

    KEGG: hsa:5364

    STRING: 9606.ENSP00000296440

    UniGene: Hs.476209