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  4. Human Phospholipidase A2,PLA2 ELISA Kit

Human Phospholipidase A2,PLA2 ELISA Kit

  • 中文名称：
    人磷脂酶A2(PLA2)酶联免疫试剂盒
  • 货号：
    CSB-E08110h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human phospholipidase A2 (PLA2) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PLA2 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PLA2 bound in the initial step.

    PLA2 plays key roles in multiple cellular responses, including phospholipid digestion & metabolism, host defense, and signal transduction. PLA2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. PLA2 is a key enzyme in the regulation of lipid mediators of the inflammatory process. Evidence has shown that PLA2 is involved in all stages of pathogen encounter, from the initial signaling to the uptake, degradation, and presentation of the antigen.

  • 别名：
    Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.45 ng/mL-30 ng/mL
  • 灵敏度：
    0.11 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %93
    Range %88-97
    1:10Average %104
    Range %100-109
    1:20Average %95
    Range %91-99
    1:40Average %85
    Range %81-92
  • 回收率：
    The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-96
    EDTA plasma (n=4)9793-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.534 2.452 2.493 2.384
    151.652 1.576 1.614 1.505
    7.51.066 1.004 1.035 0.926
    3.750.578 0.561 0.570 0.461
    1.80.387 0.401 0.394 0.285
    0.90.238 0.245 0.242 0.133
    0.450.179 0.187 0.183 0.074
    00.105 0.112 0.109 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Upon binding to the PLA2R1 receptor can regulate podocyte survival and glomerular homeostasis. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.
  • 基因功能参考文献：
    1. sPLA2-IB was correlated with the level of proteinuria in membranous nephropathy patients suggesting to be a potential biomarker for monitoring disease severity and therapeutic effects of both primary membranous nephropathy and secondary membranous nephropathy. PMID: 29649452
    2. The sPLA2 IB-PLA2R interaction stimulated podocyte apoptosis through activating ERK1/2 and cPLA2alpha and through increasing the podocyte AA content PMID: 25335547
    3. TNF-alpha-induced cPLA2 expression and PGE2 release were mediated through a Jak2/PDGFR/PI3K/Akt/p42/p44 MAPK/Elk-1 pathway in human lung epithelial cells. PMID: 24441870
    4. Exogenously added sPLA(2)-IB decreases phagocytosis of photoreceptor outer segments regardless of enzymatic activity. PMID: 22680611
    5. stimulation of three isoforms of PLA2 by thapsigargin liberates free AA that, in turn, induces capacitative calcium influx in human T-cells PMID: 12423354
    6. results indicate a selective sorting of a cell-derived cPLA2 during human cytomegalovirus maturation, which is further required for infectivity PMID: 15220446
    7. Group IB phospholipase A2 (PLA2G1B) stimulates leukotriene B4 (LTB4) production in the absence of cytochalasin B in human neutrophils. PMID: 16005851
    8. Here, we report sPLA2-IB in rat and human brain as well as in neurons in primary culture. The distribution of sPLA2-IB seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. PMID: 16392040
    9. a critical regulatory role of arachidonate reacylation that limits leukotriene biosynthesis in concert with 5-lipoxygenase and cytosolic phospholipase A(2)alpha activation PMID: 16495221
    10. Results describe the structural basis for bile salt inhibition of pancreatic phospholipase A2. PMID: 17434532
    11. MMP-2/9 production is regulated by sPLA2-IB acting as a receptor ligand to activate cPLA2 PMID: 17981679
    12. pro-hG1B forms a trimer and PROP occupies the catalytic cavity and can be self-cleaved at 37 degrees C; A new membrane-bound surface and activation mechanism are proposed based on the trimeric model of pro-hG1B PMID: 19297324

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  • 亚细胞定位：
    Secreted. Note=Secreted from pancreatic acinar cells in its inactive form.
  • 蛋白家族：
    Phospholipase A2 family
  • 组织特异性：
    Selectively expressed in pancreas, lung, liver and kidney. Also detected at lower levels in ovary and testis.
  • 数据库链接：

    HGNC: 9030

    OMIM: 172410

    KEGG: hsa:5319

    STRING: 9606.ENSP00000312286

    UniGene: Hs.992