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  4. Human Peroxidasin homolog(PXDN) ELISA kit

Human Peroxidasin homolog(PXDN) ELISA kit

  • 中文名称：
    人过氧蛋白同源物(PXDN)酶联免疫试剂盒
  • 货号：
    CSB-EL019106HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PXDN ELISA Kit was designed for the quantitative measurement of Human PXDN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.188 ng/mL-12 ng/mL and the sensitivity is 0.047 ng/mL.

  • 别名：
    PXDN ELISA Kit; KIAA0230 ELISA Kit; MG50 ELISA Kit; PRG2 ELISA Kit; VPO ELISA Kit; VPO1 ELISA Kit; Peroxidasin homolog ELISA Kit; EC 1.11.1.7 ELISA Kit; Melanoma-associated antigen MG50 ELISA Kit; Vascular peroxidase 1 ELISA Kit; p53-responsive gene 2 protein ELISA Kit
  • 缩写：
    PXDN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.188 ng/mL-12 ng/mL
  • 灵敏度：
    0.047 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PXDN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 101    
    Range % 95-105    
    1:2 Average % 97    
    Range % 92-101    
    1:4 Average % 89    
    Range % 84-93    
    1:8 Average % 94    
    Range % 90-98    
  • 回收率：
    The recovery of human PXDN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 87-98    
    EDTA plasma (n=4) 85 80-90    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    12 2.582 2.431 2.507 2.348    
    6 2.174 2.118 2.146 1.987    
    3 1.493 1.395 1.444 1.285    
    1.5 0.738 0.728 0.733 0.574    
    0.75 0.521 0.487 0.504 0.345    
    0.375 0.307 0.302 0.305 0.146    
    0.188 0.219 0.204 0.212 0.053    
    0 0.156 0.162 0.159 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation.
  • 基因功能参考文献：
    1. PXDN might play a promoter role in the proliferation, invasion and migration of ovarian cancer (OC) cells through regulating the activation of PI3K/Akt pathway. Therefore, PXDN might be regarded as a potential target for OC therapy. PMID: 29661721
    2. e showed that collagen IV crosslinks remain intact even under strongly hypoxic conditions. Our hypothesis is that during collagen IV network formation PXDN cooperates with a NOX/DUOX-independent H2O2 source that is functional also at very low ambient oxygen levels. PMID: 29573705
    3. Data indicate that VPO1 plays a critical role in regulating eNOS expression and activity by structural change and de-phosphorylation. VPO1 expression was up-regulated in Ang-treated HUVECs. Knockdown of VPO1 was su ffi cient to attenuate the Ang induced decrease in eNOS expression and activity. Moreover, the e ff ect of VPO1 on eNOS was mainly achieved by HOCl formation. PMID: 28264790
    4. Results show that PXDN expression decreases with TGF-beta1 treatment in cervical carcinoma cell lines, and is regulated by the EMT master transcription factor Snai1. This implicates PXDN in cancer development and processes that involve EMT. PMID: 29305973
    5. Data suggest that VPO1 contributes to oxidation and retention of LDL in vessel walls, and formation foam cells, indicating VPO1 as a novel potential mediator of atherosclerosis. PMID: 27167346
    6. VPO1 plays a critical role in asymmetric dimethylarginine production via H2O2-VPO1-hypochlorous acid pathways, which may contribute to endothelial dysfunction in hypertension. PMID: 27475679
    7. The kinetic data unravel the substrate specificity and mechanism of halide oxidation of human peroxidasin 1. The heme enzyme is shown to follow the halogenation cycle that is induced by the rapid H2O2-mediated oxidation of the ferric enzyme to the redox intermediate compound I. Chloride cannot act as a two-electron donor of compound I, whereas thiocyanate, iodide, and bromide efficiently restore the ferric state. PMID: 28154175
    8. PC processing of Pxdn is a key regulatory step that contributes to its function and, therefore, supports BM integrity and homeostasis. PMID: 27697841
    9. PXDN expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    10. The catalytic domain of peroxidasin and its immunoglobulin (Ig) domains are required for efficient sulfilimine bond formation. PMID: 26178375
    11. Novel mutations in PXDN cause microphthalmia and anterior segment dysgenesis PMID: 24939590
    12. Despite sequence homology at the active site and biophysical properties similar to human peroxidases, the catalytic efficiency of bromide oxidation (kcat/KM(app)) of full-length hsPxd01 is rather low but increased upon truncation. PMID: 25713063
    13. Peroxidasin is essential for eye development in the mouse. PMID: 24895407
    14. MiR-4782-3p inhibited cell proliferation in NSCLC by targeting USP14, ZEB2 and XIAP. PMID: 24556843
    15. studies demonstrate the enzymatic properties and substrate specificity of VPO1 are similar to MPO; however, significantly lower catalytic rate constants of VPO1 relative to MPO suggest the possibility of other physiologic roles for this peroxidase PMID: 22982576
    16. VPO1 is likely to participate in host defense, with bactericidal activity mediated through the generation of HOCl. PMID: 22526679
    17. Results from this study indicate that the 2p25.3 duplication disrupting PXDN and MYT1L is a potential autism-causing variant in the pedigree reported here and should receive further consideration as a candidate for autism PMID: 22157634
    18. plays a critical role in ox-LDL-induced endothelial cell apoptosis PMID: 21820048
    19. Vascular peroxidase-1 is rapidly secreted, circulates in plasma, and supports dityrosine cross-linking reactions. PMID: 21798344
    20. Homozygous mutations in PXDN cause congenital cataract, corneal opacity, and developmental glaucoma. PMID: 21907015
    21. peroxidasin secretion represents a previously unknown pathway in extracellular matrix formation with a potentially important role in the physiological and pathological fibrogenic response. PMID: 19590037

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  • 相关疾病：
    Anterior segment dysgenesis 7 (ASGD7)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Note=Enriched in the peritubular space of fibrotic kidneys.
  • 蛋白家族：
    Peroxidase family, XPO subfamily
  • 组织特异性：
    Expressed at higher levels in heart, lung, ovary, spleen, intestine and placenta, and at lower levels in liver, colon, pancreas, kidney, thymus, skeletal muscle and prostate. Expressed in tumors such as melanoma, breast cancer, ovarian cancer and glioblas
  • 数据库链接：

    HGNC: 14966

    OMIM: 269400

    KEGG: hsa:7837

    STRING: 9606.ENSP00000252804

    UniGene: Hs.332197