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  4. Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

  • 中文名称：
    人肽脯氨酰顺反异构酶C(PPIC)酶联免疫试剂盒
  • 货号：
    CSB-EL018473HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PPIC ELISA Kit was designed for the quantitative measurement of Human PPIC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    Cyclophilin C ELISA Kit; CYPC ELISA Kit; MGC3673 ELISA Kit; Parvulin ELISA Kit; Peptidyl prolyl cis trans isomerase C ELISA Kit; Peptidyl-prolyl cis-trans isomerase C ELISA Kit; Peptidylprolyl isomerase C (cyclophilin C) ELISA Kit; peptidylprolyl isomerase C ELISA Kit; PPIase C ELISA Kit; ppiC ELISA Kit; PPIC_HUMAN ELISA Kit; Rotamase C ELISA Kit
  • 缩写：
    PPIC
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PPIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-104
    1:2 Average % 91
    Range % 82-98
    1:4 Average % 103
    Range % 98-108
    1:8 Average % 87
    Range % 82-98
  • 回收率：
    The recovery of human PPIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 85-98
    EDTA plasma (n=4) 111 105-115
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.651 2.656 2.654 2.497
    750 2.322 2.251 2.287 2.130
    375 1.645 1.655 1.650 1.493
    187.5 1.002 0.974 0.988 0.831
    94 0.587 0.544 0.566 0.409
    47 0.389 0.401 0.395 0.238
    23.5 0.262 0.245 0.254 0.097
    0 0.156 0.157 0.157 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
  • 基因功能参考文献：
    1. Independent validation with glioma patients tissue (n = 70) and normal brain tissue (n = 19) found PPIC, EMP3 and CHI3L1 were up-regulated in glioma tissue. Survival value validation showed that the three genes correlated with patient survival by Kaplan-Meir analysis, including grades, age and therapy. PMID: 27801851
    2. Cyclophilin C participates in cytomegalovirus US2 protein-mediated degradation of major histocompatibility complex class I molecules. PMID: 26691022
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Cyclophilin-type PPIase family
  • 组织特异性：
    Expressed in kidney, skeletal muscle, pancreas, heart, lung, liver and to a lower extent in brain.
  • 数据库链接：

    HGNC: 9256

    OMIM: 123842

    KEGG: hsa:5480

    STRING: 9606.ENSP00000303057

    UniGene: Hs.110364