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  4. Human Peptidase inhibitor 16(PI16) ELISA kit

Human Peptidase inhibitor 16(PI16) ELISA kit

  • 中文名称：
    人肽酶抑制剂16酶联免疫试剂盒
  • 货号：
    CSB-EL017951HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PI16 ELISA Kit was designed for the quantitative measurement of Human PI16 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-10 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    Cripi ELISA Kit; CRISP 9 ELISA Kit; CRISP-9 ELISA Kit; CRISP9 ELISA Kit; Cysteine rich protease inhibitor ELISA Kit; Cysteine rich secretory protein 9 ELISA Kit; Cysteine-rich secretory protein 9 ELISA Kit; Microseminoprotein beta binding protein ELISA Kit; MSMBBP ELISA Kit; Peptidase inhibitor 16 ELISA Kit; PI-16 ELISA Kit; PI16 ELISA Kit; PI16_HUMAN ELISA Kit; Protease inhibitor 16 ELISA Kit; PSP94 binding protein ELISA Kit; PSP94-binding protein ELISA Kit; PSPBP ELISA Kit
  • 缩写：
    PI16
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-10 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<20%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PI16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 83-98
    1:2 Average % 95
    Range % 88-105
    1:4 Average % 90
    Range % 84-105
    1:8 Average % 95
    Range % 89-100
  • 回收率：
    The recovery of human PI16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-105
    EDTA plasma (n=4) 95 86-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    10 2.254 2.266 2.260 　
    5 1.342 1.354 1.348 　
    2.5 0.662 0.669 0.666 　
    0.625 0.197 0.211 0.204 　
    0.312 0.099 0.102 0.101 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May inhibit cardiomyocyte growth.
  • 基因功能参考文献：
    1. at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
    2. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation PMID: 22533972
    3. A new blood protein PSP94-binding protein (PSPBP) is identified that binds prostate secretory protein of 94 amino acids (PSP94). PMID: 15344909
    4. a novel and independent prognostic markers following radical prostatectomy for prostate cancer. PMID: 17062675
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    CRISP family
  • 组织特异性：
    Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.
  • 数据库链接：

    HGNC: 21245

    KEGG: hsa:221476

    STRING: 9606.ENSP00000362778

    UniGene: Hs.25391