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Human POTE ankyrin domain family member E(POTEE) ELISA kit

  • 中文名称:
    人POTE锚蛋白域家庭成员E(POTEE)酶联免疫试剂盒
  • 货号:
    CSB-EL740863HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human POTEE ELISA Kit was designed for the quantitative measurement of Human POTEE protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名:
    POTEE ELISA Kit; A26C1A ELISA Kit; POTE2 ELISA Kit; POTE ankyrin domain family member E ELISA Kit; ANKRD26-like family C member 1A ELISA Kit; Prostate ELISA Kit; ovary ELISA Kit; testis-expressed protein on chromosome 2 ELISA Kit; POTE-2 ELISA Kit
  • 缩写:
    POTEE
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    25 pg/mL-1600 pg/mL
  • 灵敏度:
    6.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human POTEE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 98
    Range % 93-103
    1:4 Average % 102
    Range % 98-106
    1:8 Average % 91
    Range % 86-97
  • 回收率:
    The recovery of human POTEE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 96-108
    EDTA plasma (n=4) 89 83-94
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.786 2.934 2.860 2.729
    800 2.214 2.341 2.278 2.147
    400 1.425 1.467 1.446 1.315
    200 0.743 0.754 0.749 0.618
    100 0.456 0.446 0.451 0.320
    50 0.327 0.312 0.320 0.189
    25 0.216 0.211 0.214 0.083
    0 0.132 0.130 0.131  
  • 数据处理:
  • 货期:
    3-5 working days

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