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  4. Human P2X purinoceptor 7(P2RX7) ELISA kit

Human P2X purinoceptor 7(P2RX7) ELISA kit

  • 中文名称：
    人P2X嘌呤受体7(P2RX7)酶联免疫试剂盒
  • 货号：
    CSB-EL017325HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human P2RX7 ELISA Kit was designed for the quantitative measurement of Human P2RX7 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    ATP receptor ELISA Kit; P2rx7 ELISA Kit; P2RX7_HUMAN ELISA Kit; P2X purinoceptor 7 ELISA Kit; P2X7 ELISA Kit; P2Z receptor ELISA Kit; Purinergic receptor ELISA Kit; purinergic receptor P2X, ligand gated ion channel, 7 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human P2RX7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %91-105
    1:2Average %90
    Range %85-97
    1:4Average %93
    Range %89-102
    1:8Average %95
    Range %88-104
  • 回收率：
    The recovery of human P2RX7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9789-104
    EDTA plasma (n=4)9689-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    16002.016 1.898 1.957 1.871
    8001.152 1.115 1.134 1.048
    4000.677 0.647 0.662 0.576
    2000.363 0.374 0.369 0.283
    1000.241 0.252 0.247 0.161
    500.188 0.200 0.194 0.108
    250.127 0.134 0.131 0.045
    00.085 0.087 0.086
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

你能告诉我P2X7　ELISA kit识别的是哪个表位，或者用了哪个免疫原来提高AB吗?

A:
试剂盒CSB-EL017325HU的免疫原为293细胞源性重组蛋白。我们没有检测到抗体上的表位映射，因此无法提供识别区域信息。 包被抗体:小鼠单克隆抗体　　 检测抗体:大鼠单克隆抗体 本试剂盒参考:　https://www.uniprot.org/uniprotkb/Q99572/

靶点详情

  • 功能：
    Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.
  • 基因功能参考文献：
    1. Results suggest the pathophysiological role of purinergic receptor P2X7 (P2X7) in pancreatic disease and recovery. PMID: 29683976
    2. The P2x7 ion channel receptor was regularly absent in both the periodontal ligament and dental tissues PMID: 29289709
    3. These results suggest that ERK pathway is involved in the proliferation and migration of glioma cells induced by P2X7R activation. PMID: 29546069
    4. Increased P2X7 receptor expression in monocytes are manifestations of chronic inflammation in the early stages of chronic kidney disease. PMID: 29375053
    5. the P2X7R rs3751143 and ER-alpha PvuII two-locus interaction confers a significantly high susceptibility to osteoporosis in Chinese postmenopausal women. PMID: 28884379
    6. that the P2X7R rs3751143 functional polymorphism might contribute to osteoporosis susceptibility in Chinese postmenopausal women PMID: 28497417
    7. indicating the involvement of P2X7R in the growth of esophageal squamous cell carcinoma PMID: 28397110
    8. Data show that Pr2x7 gene deletion protects from HFD-induced NASH, possibly through blunted activation of NLRP3 inflammasome. PMID: 29270247
    9. this study demonstrates that P2X7 is not essential for development of imiquimod -induced psoriasis-like inflammation PMID: 28597172
    10. This study revealed that the P2X7R/NLRP3 pathway plays important roles in IL-1beta secretion and inhibition of Toxoplasma gondii proliferation in small intestinal epithelial cells. PMID: 29291748
    11. P2X7R contributes to the progression of spinal TB. The P2X7 -762C>T and 489C>T polymorphisms are correlated with susceptibility to spinal TB. Carrying the -762CC genotype and 489T allele increases the risk of developing spinal TB in a Southern Chinese Han population. PMID: 29158203
    12. Increased expression of P2X7R in peripheral blood mononuclear cells from patients with rheumatoid arthritis.P2X7R role in th17 cells differentiation. PMID: 27775097
    13. results show that P2X7 is a scavenger receptor with important function in the CNS but its phagocytic function has features distinct from its pore function. Both P2X7 pore formation and P2X7-mediated phagocytosis should be considered in the design of new P2X7 antagonists for the treatment of CNS diseases. PMID: 29329985
    14. This pathway provides new insight into factors that increase dynamic blebbing and identifies new targets, such as P2X7, that can be used to improve the culture of cells with therapeutic potential. PMID: 28672157
    15. a major role for P2X7R and P2Y11R in ATP-mediated inhibition of tumor-derived endothelial cell migration, is reported. PMID: 27586846
    16. To investigate the correlation between P2X7R, NLRP3 and cell growth, NLRP3 was silenced in THP-1 cells, a leukemic cell line that natively expresses both NLRP3 and P2X7R. NLRP3 silencing enhanced P2X7R expression and promoted growth. On the contrary, NLRP3 overexpression caused accelerated apoptosis. PMID: 27221966
    17. Genetic polymorphisms of the P2X7 gene associated with susceptibility to and prognosis of pulmonary tuberculosis. PMID: 28495473
    18. The results suggest that although the 1068 G>A polymorphism of the P2RX7 gene is associated with an increased beta-cell function and IL-1Ra release in type 2 diabetes patients, the glycemic control is not significantly affected by the presence of this SNP. PMID: 29425823
    19. These data suggest that P2X7R activation may contribute to the high prevalence of kidney disease found in diabetics. PMID: 28434946
    20. In conclusion, the results suggest that P2X7R may promote IL-6, IL-8 and MCP-1 production and secretion and contribute to the invasion and adhesion of craniopharyngiomas to the surrounding tissue. PMID: 28389503
    21. P2X7R is expressed by three malignant pleural mesothelioma cell lines established from MPM patients but not by mesothelial cells from healthy subjects PMID: 27391069
    22. A total of 163 patients and 201 health controls were enrolled in this study and polymorphisms of NLPR1, NLRP3, and P2X7R genes were detected by PCR..Our study demonstrated the potentially significant role of NLRP1 rs878329 (G>C) in developing susceptibility to the partial seizures in a Chinese Han population. PMID: 28503575
    23. Inheritance of the C/C genotype at position 253 in the P2RX7 gene may contribute to the risk of HPV-16 associated cervical squamous cell carcinoma in Taiwanese women. PMID: 27779103
    24. This study demonstrated that ischemic preconditioning Induces Long-Lasting Increase in P2X7 Receptor in Astrocytes. PMID: 28063215
    25. The link is being discussed between P2X7R signaling and TG2 export, a pathway that has been recently discovered and tied extracellular protein modifications into the danger signal-mediated innate immune response. (Review) PMID: 27562793
    26. P2X7R is involved in propagation of mechanically-induced intercellular signaling in addition to the known mechanisms involving calcium signaling via P2Y2 receptors and gap junction. PMID: 27856358
    27. this study shows that P2X7R is directly involved in the modulation of the antiviral and inflammatory process that occurs during Dengue virus infection in vitro PMID: 26969484
    28. These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation. PMID: 29061307
    29. The results indicate that P2X7 receptors may play a significant role in contributing to the unwanted activation of mast cells in chronic inflammatory conditions where extracellular ATP levels are elevated PMID: 26910735
    30. In vitro and in vivo results provide support for the involvement of an oxidative stress through P2X7 receptor activation and mitochondrial dysfunction in the pathophysiology of oxaliplatin-induced neuronal injury and likely to painful neuropathy. PMID: 23826152
    31. Eight Single nucleotide polymorphisms loci, including rs1653624, rs10160951, rs1718119, rs7958316, rs16950860, rs208294, rs17525809 and rs2230912, were screened and detected, and rs1653624, rs7958316 and rs17525809 were associated with gout arthritis. P2X7R function associated single nucleotide polymorphisms may be related to gouty arthritis. PMID: 28797095
    32. The loss-of-function SNP rs2230911 in P2X7, that negatively affect NLRP3-inflammasome activation, confers susceptibility toward active pulmonary tuberculosis in Brazilian Amazon cohort. PMID: 27101784
    33. this review discusses P2X7R structure and its contribution to inflammation and host defense PMID: 28723547
    34. P2X7R expression was correlated with enhanced tumor grade and metastasis in colorectal carcinoma patients. PMID: 28412208
    35. Our present study shows that loss of function of the P2X7 receptor in mice induces retinal changes representing characteristics of early age-related macular degeneration. PMID: 28628761
    36. Single nucleotide polymorphism in P2X7R gene is associated with pulmonary non-tuberculous mycobacterial disease. PMID: 28233049
    37. Our results reveal that P2RX7 rs2230911 may be associated with primary gout risk in a Chinese Han male population and allele G may be a susceptibility factor for primary gout. PMID: 28243797
    38. We think that the unbiased reader will follow our argumentation on astrocytic or microglial P2X7Rs being the primary targets of pathologically high extracellular ATP concentrations, although a neuronal localization of these receptors cannot be fully excluded either. [review] PMID: 28747388
    39. Together, this body of research suggests that P2X7R may constitute an important therapeutic target for a variety of neurological disorders. PMID: 28747389
    40. In addition, purinergic receptor P2X, ligand-gated ion channel 7 (P2X7) was downregulated in CD36-knockdown 3T3-L1 cells, suggesting that the suppression of CD36 attenuates adipogenesis via the P2X7 pathway in 3T3-L1 cells. PMID: 28712872
    41. analysis of the M1/M2 functional imprinting of primary microglia and the role played by P2X7 and miR-125b in amyotrophic lateral sclerosis microglia activation [review] PMID: 28090150
    42. Study observed a downregulation of purinergic P2X7 receptors in peripheral blood mononuclear cells of amyotrophic lateral sclerosis (ALS) patients compared to controls. PMID: 27453058
    43. Data suggest that specific serine residues in purinergic receptors play key roles in both agonist binding and receptor sensitization/desensitization: in rat P2rx7, point mutation F288S (phenylalanine 288 > serine) results in a slower rate of ATP binding/unbinding and stabilization of non-sensitized receptor states; in human P2RX7, Y288F (but not Y288S) results in a "rat-like" receptor with a fast deactivation rate. PMID: 28616989
    44. This study showed that the ATP-gated P2X7 receptor is upregulated in experimental epilepsy and resected hippocampus from epilepsy patients. PMID: 27251615
    45. ATP release by Muller cells and P2X7 upregulation in RECs. These findings are likely of in vivo relevance since CD40 upregulates P2X7 in RECs in diabetic mice and CD40 is known to be required for retinal capillary degeneration. PMID: 27893093
    46. Our study revealed a significant association between three P2X7R gene polymorphisms and pulmonary tuberculosis in a Tibetan Chinese population. PMID: 27672203
    47. circadian oscillations of intracellular calcium depend on the activation of purinergic P2X7 receptors PMID: 28139817
    48. the biochemical and molecular knowledge of P2X7R in IFCD is useful to shed further light about the participation of purinergic system in the pathogenesis of CD, and consequently, may help to avoid the progression of disease. PMID: 28062289
    49. The P2X7R rs3751142 genetic variants was not implicated in the development of gout in the male Korean population. However, we found that in a pair-wise comparison of the CA/TT P2X7R and CARD8 genotype combination was shown to have an increased trend for the risk of gout. PMID: 27550484
    50. Five gene polymorphisms, -762C>T (rs2393799), 946G>A (rs28360457), 1513A>C (rs3751143), 1068G>A (rs1718119), and 1096C>G (rs2230911), were selected. Our results suggest that 3 of the 5 polymorphisms of P2RX7 described above (1513A>C, 946G>A, and 1068G>A) are significantly associated with Hepatocellular Carcinoma susceptibility in a Chinese Han population. PMID: 27272229

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    P2X receptor family
  • 组织特异性：
    Widely expressed with highest levels in brain and immune tissues.
  • 数据库链接：

    HGNC: 8537

    OMIM: 602566

    KEGG: hsa:5027

    STRING: 9606.ENSP00000442349

    UniGene: Hs.729169