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Human P-Selectin ELISA kit

  • 中文名称：
    人P选择素(P-Selectin/CD62P)酶联免疫试剂盒
  • 货号：
    CSB-E04708h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human P-Selectin (SELP) ELISA kit is suitable for the quantitative determination of human SELP in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

    SELP is generated by activated platelets and endothelial cells and then stored in the α granules of platelets and in the Weibel-Palade bodies of endothelial cells (EC). It plays a critical role in the initial adhesion and rolling of platelets and leukocytes to sites of injury during inflammation via its receptor PSGL-1. SELP is also involved in hemostasis and thrombosis through PSGL-1 signaling in leukocytes and platelets and GPIbα in platelets. It is necessary for the progression of atherosclerosis.

  • 别名：
    Antigen CD62 ELISA Kit; CD62 ELISA Kit; CD62 antigen-like family member P ELISA Kit; CD62P ELISA Kit; GMP 140 ELISA Kit; GMP-140 ELISA Kit; GMP140 ELISA Kit; GMRP ELISA Kit; Granule membrane protein 140 ELISA Kit; Granulocyte membrane protein ELISA Kit; GRMP ELISA Kit; LECAM 3 ELISA Kit; LECAM3 ELISA Kit; Leukocyte endothelial cell adhesion molecule 3 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LYAM3_HUMAN ELISA Kit; P Selectin ELISA Kit; P-selectin ELISA Kit; PADGEM ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; Platelet alpha granule membrane protein ELISA Kit; PSEL ELISA Kit; Selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit; SELP ELISA Kit
  • 缩写：
    P-Selectin/CD62P
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.9 ng/mL-60 ng/mL
  • 灵敏度：
    0.225 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %86
    Range %80-92
    1:10Average %97
    Range %91-105
    1:20Average %98
    Range %92-110
    1:40Average %95
    Range %86-98
  • 回收率：
    The recovery of human P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9687-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    602.305 2.368 2.337 2.269
    301.795 1.805 1.800 1.732
    151.146 1.187 1.167 1.099
    7.50.653 0.689 0.671 0.603
    3.750.344 0.358 0.351 0.283
    1.80.245 0.264 0.255 0.187
    0.90.110 0.116 0.113 0.045
    00.066 0.069 0.068 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
  • 基因功能参考文献：
    1. Suggest role for soluble P-selectin in the diagnosis of venous thromboembolism. PMID: 30093507
    2. plasma level predictive of high residual platelet reactivity during maintenance-phase dual antiplatelet therapy PMID: 29334682
    3. Kidney transplant has a significant restorative effect on platelet aggregation and coagulation functions independent of platelet activation, as evaluated with P-selectin levels. PMID: 27267514
    4. In Chinese Han population cardiovascular disease (CVD) risk is significantly higher in carriers with rs1800805-A allele (GA and AA) in the selectin P gene (SELP) than those with GG genotype. CVD risk is the highest for rs1800805-GA or AA carriers with additional genetic polymorphisms 1800796-GC or CC in interleukin 6 gene. PMID: 28819827
    5. VWF, GMP-140, ADAMTS13 and the cerebral vasospasm, delayed cerebral ischemia, tumor diameter and prognosis of aneurysmal subarachnoid hemorrhage patients are closely related PMID: 29077161
    6. The highest level of sP-selectin in colorectal patients was observed in patients with metastases to the liver (stage IV), and was significantly higher than in patients without metastases (stage I/II) and with lymph node metastases (stage III), p = .02. P-selectin plays an important role in the progression of CRC PMID: 27775438
    7. Serum P-selectin levels are not altered in cystic fibrosis patients. PMID: 28646244
    8. miR-223, miR-26b, miR-126 and miR-140 are expressed at a lower level in platelets and megakaryocytes in type 2 diabetics causing up-regulation of P2RY12 and SELP mRNAs that may contribute to adverse platelet function. PMID: 27975100
    9. coronary thrombi in STEMI patients resistant to fibrinolysis are characterised by higher fibrin, P-selectin and VWF content than lysis-sensitive thrombi PMID: 26962963
    10. P-Selectin and ICAM-1 have roles in mediating THP-1 monocyte adhesion PMID: 28262902
    11. Transgenic expression of SELP is atherogenic in Apoe(-/-) mice, suggesting that P-selectin contributes to atherogenesis. PMID: 27102967
    12. These results provide a novel insight into the mechano-chemical regulation mechanism for P-selectin-induced calcium signaling of neutrophils in flow. PMID: 28097631
    13. Data indicate no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with fetal growth restriction (FGR). PMID: 28358707
    14. Study concluded that the genotype and allele frequencies of SELP S290N were not significant different between the acute ischemic stroke case group and the control group as well as between each subtype group and the control group PMID: 27423570
    15. High SELP expression is associated with Inflamed Atherosclerotic Plaque. PMID: 27237221
    16. Circulating P-selectin levels were closely associated with endocan levels in obese children and adolescents with non-alcoholic fatty liver disease. PMID: 28318371
    17. Primary Aldosteronism is related to platelet activation, expressed as higher plasma values of soluble CD40L and soluble P-selectin values. PMID: 27101095
    18. Immobilized P-selectin-induced calcium signaling of HL-60 cells is P-selectin concentration- and mechanical force-dependent PMID: 28155729
    19. results found that LMWF protected the renal function in diabetic nephropathy rats and alleviated inflammation through the modulation of P-selectin and inflammatory cytokines. LMWF may have therapeutic potential against diabetic nephropathy. PMID: 27234491
    20. The study establishes that thrombotic-inflammatory pathways enhancing P-selectin exposure unrelated to treatment might be activated in patients, while the event rate remained lowered, and hence, treatment strategies should be inclusive to control these factors. PMID: 27406211
    21. This study could not support the potential benefit of the high-dose vitamin D on plasma level of P-selectin and hs-CRP in patients with VTE. PMID: 25601896
    22. The level of d-dimer, P-selectin, and platelet count might be good candidate predictive markers for PVT in patients with cirrhotic PHT after devascularization. The combined test of the 3 markers can increase the value of prediction. PMID: 25633343
    23. deletion of P-sel disrupted megakaryocyte/neutrophil interactions in spleen, reduced TGF-beta content, and corrected the hematopoietic stem cells distribution that in Gata1(low) mice, as in primary myelofibrosis patients, is abnormally expanded in spleen. PMID: 26439305
    24. Elevated plasma P-selectin autoantibodies may play a role in the pathogenesis of thrombocytopenia in pSS patients. PMID: 26613867
    25. The addition of sialic acid enhances the affinity of CD15 to P-selectin and augments the cell adhesion to blood vessel endothelial cells. PMID: 26418972
    26. This study provides a better understanding of the biology of P-selectin and PSGL-1 and their roles in dissemination and resensitization of Multiple myeloma treatment. PMID: 26539491
    27. Data (including data from studies in transgenic mice) suggest that sequence variations limited to 1.4-kb proximal promoter region account for most differences in expression of murine Selp and human SELP genes. PMID: 26631722
    28. P-selectin gene polymorphisms and haplotypes can contribute to proliferative diabetic retinopathy development. PMID: 26344728
    29. P-selectin polymorphism of -825T/C is associated with the risk of pulmonary hypertension in congenital heart defects, and may be one of its risk factors. PMID: 26261613
    30. Soluble P-selectin was upregulated I ankylosing spondylitis patients could predict prognosis. PMID: 26261626
    31. P-selectin has a role in endothelial progenitor cells' inhibition of platelet function PMID: 25948279
    32. s. Leukocyte filtration does not affect the CD62P surface expression of apheresis platelets stored for up to 2 days, which indicates that leukocyte filtration does not damage the activation of apheresis platelets within the retention period PMID: 26125797
    33. Plasmodium falciparum MSP7 and P-selectin were shown to bind each other directly via the N-terminus of PfMSP7 and the P-selectin C-type lectin and EGF-like domains. PMID: 26045295
    34. CD62P levels may be useful as a sensitive clinical marker for the early detection of DWMLs [deep white matter lesions]with cognitive decline. PMID: 26388436
    35. time changes of sCD40L over 1 month after myocardial infarct onset were associated with G894T eNOS polymorphism and with the VEGF concentrations, but not to the platelet CD62P expression PMID: 26279254
    36. Platelet-bound P-selectin appears to be the major determinant of leukocyte-platelet interactions in patients with cardiovascular disease. PMID: 25428141
    37. Inclacumab was well tolerated by the majority of subjects after single intravenous infusion and demonstrated pharmacological activity against both cell expressed P-selectin and soluble P-selectin. PMID: 25714598
    38. There was a larger number of endothelial cells expressing SELP in the lepromatous form compared to the tuberculoid form of leprosy skin lesions. PMID: 26051909
    39. The adhesion molecules CD62P and CD44 play an important role in the pathogenesis of bronchiolitis. PMID: 26575878
    40. SELp expression on platelets of type 2 diabetes patients without prior ischemic events was similar to that of controls. PMID: 26068309
    41. Observe ethnic heterogeneity in the association of P-selectin and risk of coronary heart disease. PMID: 25744700
    42. P-selectin is significantly associated with the development of peripheral artery disease PMID: 25682040
    43. SELP polymorphisms are associated with cachexia development in pancreatic cancer patients. PMID: 25238546
    44. Data indicate that the simultaneous assay of activated platelets (aPLTs) and platelet-activating capacity by P-selectin (CD62P) detection using whole blood treated with the K2-EDTA anticoagulant was useful for the monitoring of antiplatelet drugs. PMID: 22862794
    45. Diesel exhaust exposure during exercise induces platelet activation as illustrated by a dose-response increase in the release of CD62P and CD63. PMID: 25297946
    46. Soluble P-selectin promotes acute myocardial infarction onset but not severity. PMID: 25384966
    47. P-selectin may not be a predicted risk factor for deep vein thrombosis after total hip arthroplasty PMID: 25057500
    48. Minor allele homozygotes for the variant rs6128 were less likely to develop diabetic retinopathy PMID: 25794792
    49. expression of P-selectin and P-selectin glycoprotein ligand-1, forming an auto-augmented loop of porcine aortic endothelial cells and platelet activation PMID: 25405250
    50. von Willebrand factor and IL-6, but not P-selectin in the peripheral and cardiac radiofrequency catheter ablation of atrial fibrillation circulation are affected by PMID: 25390649

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  • 相关疾病：
    Ischemic stroke (ISCHSTR)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Selectin/LECAM family
  • 组织特异性：
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • 数据库链接：

    HGNC: 10721

    OMIM: 173610

    KEGG: hsa:6403

    STRING: 9606.ENSP00000263686

    UniGene: Hs.73800