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  4. Human Orexin B,OX-B ELISA Kit

Human Orexin B,OX-B ELISA Kit

  • 中文名称：
    人食欲素/阿立新B(OX-B)酶联免疫试剂盒
  • 货号：
    CSB-E09863h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human OX-B ELISA Kit was designed for the quantitative measurement of Human OX-B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 缩写：
    OX-B
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 107    
    Range % 95-111    
    1:2 Average % 100    
    Range % 93-104    
    1:4 Average % 94    
    Range % 86-97    
    1:8 Average % 100    
    Range % 92-104    
  • 回收率：
    The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 84-95    
    EDTA plasma (n=4) 108 97-112    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.312 2.378 2.345 2.212    
    2000 1.386 1.346 1.366 1.233    
    1000 0.820 0.812 0.816 0.683    
    500 0.523 0.504 0.514 0.381    
    250 0.370 0.356 0.363 0.230    
    125 0.278 0.268 0.273 0.140    
    62.5 0.209 0.203 0.206 0.073    
    0 0.132 0.134 0.133 　    
  • 数据处理：
  • 货期：
    3-5 working days

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