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  4. Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit

Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit

  • 中文名称：
    人神经型一氧化氮合成酶(nNOS)酶联免疫试剂盒
  • 货号：
    CSB-E13872h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human nNOS ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human nNOS in multiple samples, including serum, plasma, and cell culture supernates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the nNOS levels in the sample and is measured at 450 nm through a microplate reader.

    nNOS, also called NOS1, is predominantly localized in the macula densa, neurons, Bowman's capsule, and collecting duct, where it takes part in the control of glomerular hemodynamics, renin release, and sodium excretion. NOS1 is the predominant nitric oxide (NO)-generating enzyme highly enriched in the brain. It is known to mediate multiple functions, ranging from learning and memory development to maintaining synaptic plasticity and neuronal development, Alzheimer's disease (AD), psychiatric disorders, and behavioral deficits. NOS1 is the major regulator of cardiac function and intracellular Ca2+ fluxes. Considering the physiological actions of NOS1 in the kidney, this enzyme may be involved in the pathogenesis of renal hemodynamic changes associated with diabetes.

  • 别名：
    2310005C01Rik ELISA Kit; BNOS ELISA Kit; Constitutive NOS ELISA Kit; EC 1.14.13.39 ELISA Kit; IHPS 1 ELISA Kit; IHPS1 ELISA Kit; N-NOS ELISA Kit; NC-NOS ELISA Kit; neuronal Nitric Oxide Synthase ELISA Kit; Neuronal NOS ELISA Kit; Nitric oxide synthase ; neuronal; included ELISA Kit; Nitric oxide synthase 1 (neuronal) ELISA Kit; Nitric oxide synthase 1 ELISA Kit; Nitric oxide synthase; brain ELISA Kit; Nitric oxide synthase; penile neuronal; included ELISA Kit; NNOS ELISA Kit; NO ELISA Kit; NOS 1 ELISA Kit; NOS ELISA Kit; NOS type I ELISA Kit; NOS-I ELISA Kit; NOS1 ELISA Kit; NOS1_HUMAN ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    0.156 IU/mL-10 IU/mL
  • 灵敏度：
    0.039 IU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human nNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %87
    Range %82-90
    1:10Average %105
    Range %101-110
    1:20Average %83
    Range %80-86
    1:40Average %97
    Range %91-103
  • 回收率：
    The recovery of human nNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)10095-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    IU/mlOD1OD2AverageCorrected
    102.902 2.963 2.933 2.743
    52.313 2.365 2.339 2.149
    2.51.469 1.465 1.467 1.277
    1.250.878 0.822 0.850 0.660
    0.6250.568 0.545 0.557 0.367
    0.3120.407 0.435 0.421 0.231
    0.1560.314 0.326 0.320 0.130
    00.192 0.187 0.190
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 基因功能参考文献：
    1. Raynaud's phenomenon is associated with variation in gene NOS1 PMID: 29698501
    2. A meta-analysis was performed for the association of rs2682826, rs3782206, rs499776, rs3782219, rs41279104, rs3782221, rs1879417, rs4767540, rs561712, and rs6490121 polymorphisms with schizophrenia. rs3782206 showed a strong association with schizophrenia in allelic, homozygote, dominant, and recessive models in Asians. In Caucasians, rs499776 was associated in homozygote, dominant, and recessive models. PMID: 28795310
    3. Based on a quantitative comparison of their dendritic characteristics, the NOS1 neurons of humans and rats displayed a statistically significant difference PMID: 28720408
    4. The neuronal NOS exon 7 in the experimental group has a higher ratio of G/T and T/T genotypes than the control group, and also that the neuronal NOS intron 4 in the experimental group has a significantly higher a/b genotype than the control group. This suggests that the neuronal NOS gene mutation could be related to the development of osteonecrosis of the femoral head. PMID: 28770971
    5. High NOS1 expression is associated with Dilated Hearts. PMID: 27481317
    6. NOS1-ex1f VNTR is associated with white matter microstructure in females with ADHD and healthy females. PMID: 28589541
    7. selected nNOS polymorphisms do not significantly contribute to Parkinson's disease risk in north Indian population. PMID: 26081147
    8. Lysophosphatidylcholine induced nNOS uncoupling and nNOS(Ser852) phosphorylation, reduced NO and H2O2 production and improved superoxide production by modulating ERK1/2 activity in endothelial cells. PMID: 28235709
    9. NOS1 (missense variant rs79487279) was associated with bipolar disorder. PMID: 28195573
    10. Data suggest that NOS1 and NOS2 play roles in stress-induced surge in nitric oxide (NO) production; NO serves as mediator for development of secondary neurological disorders associated with stress such as anxiety and anxiety disorders. [REVIEW] PMID: 28061969
    11. nNOS mediates the human coronary vasodilator response to mental stress, predominantly through actions at the level of coronary resistance vessels. PMID: 28646032
    12. These findings highlight a key role of the TLR4-NOS1-AP1 signaling axis in regulating macrophage polarization PMID: 28013342
    13. Association between NOS1 and PTSD severity and stress was found. NOS1 was associated with resilience. PMID: 28465168
    14. Data show that metastasis associated 1 (MTA1) represses neuronal nitric oxide synthase (nNOS) expression upon oxygen glucose deprivation (OGD)-induced oxidative stress. PMID: 27603575
    15. Superoxide generation from NOS1 splice variants and its potential involvement in redox signal regulation has been described. PMID: 28126743
    16. expression of neuronal NOS heterodimers in insect cells, where we take advantage of an exogenous heme-triggered chaperone-assisted assembly process, provides an approximately 43% yield in heterodimeric NOS. PMID: 27487179
    17. NOS1 ex1f-VNTR is associated with anxiety-related traits. PMID: 26746182
    18. The NOS1 exon18 variants might play a role in the risk of Parkinson's disease (Meta-Analysis) PMID: 27749554
    19. nNOS gene variants contribute to OCD pathogenesis. PMID: 27739347
    20. Findings demonstrate that the upregulation of cardiac NOS1 is not accompanied by an increase in NOS activity; it is partially translocated to the sarcolemma in ischemic hearts with direct relationship between its protein levels and systolic ventricular function. These results indicate that NOS1 may be significant in the pathophysiology of human ischemic heart disease. PMID: 27041589
    21. The found of this study suggested that possible roles of NOS1 gene in working memory performance in ADHD patients PMID: 26233433
    22. NOS1 rs7977109 and rs693534 genotypes and allelic variants are not associated with the risk for migraine in Caucasian Spanish people PMID: 26283425
    23. These results of this study suggested that SNAP25 and NOS1 genotypes influence ADHD symptoms only in adults with ADHD. PMID: 26821215
    24. CoCl2 increased nNOS expression and NO production in human neuroblastoma SK-N-SH cells by increasing HIF-1a expression and binding to a a hypoxia response element in the nNos promoter. PMID: 26458913
    25. polymorphisms in the gene may be useful in identifying women at risk for osteoporosis. PMID: 25871004
    26. DLC1 binding to nNOS-calmodulin complex does not affect the electron transport from the reductase to the oxygenase domain. PMID: 26923072
    27. We conclude that DNM2 is a novel negative regulator of NO production in mouse collecting ducts. PMID: 26791826
    28. analysis of the interaction between nNOS and dystrophin repeats 16 and 17 PMID: 26378238
    29. the first structure of human neuronal nitric oxide syntahse PMID: 25286850
    30. This study first-time showed highest Red Blood Cell (RBC)-Nitric Oxide Synthase activation and nitric oxide production in old RBC. PMID: 25902315
    31. Results suggested a link between NOS1 gene polymorphism at rs3782206 and cognitive functions and their neural underpinnings at the right inferior frontal gyrus PMID: 25490993
    32. Tetrahydrobiopterin:dihydrobiopterin ratio is lower in tumor tissues and, as a consequence, NOS activity generates more peroxynitrite and superoxide anion than nitric oxide, resulting in important tumor growth-promoting and antiapoptotic signaling properties. PMID: 25724429
    33. Study found no significant differences either in the frequencies of the NOS1 rs7977109 and rs693534 genotypes or in the frequencies of the allelic variants of these SNPs in restless legs syndrome patients when compared with healthy controls PMID: 25300364
    34. Studied and determined the localisation of neuronal NOS (nNOS) in human airway epithelium. PMID: 25460324
    35. T allele of rs2293050 and A allele of rs2139733 in nNOS gene may contribute to increased susceptibility of LAA-caused ischemic stroke in Han Chinese PMID: 24668187
    36. Results suggest that NOS1 SNPs interact with exposure to economic and psychosocial stressors to alter individual's susceptibility to depression PMID: 24917196
    37. we discuss the idea that post-translational modifications of Ca2 + regulatory proteins via aberrant neuronal nitric oxide synthase (NOS1)-mediated nitroso-redox balance contribute to contractility defects in Heart failure PMID: 24801117
    38. 2-methoxyestradiol specifically affects neuronal nitric oxide synthase and augments 3-nitrotyrosine level leading to osteosarcoma and immortalized hippocampal cell death. PMID: 25170949
    39. Increased cellular expression of nNOS was accompanied by elevated NO generation in brains of heroin addicts and may have contributed to some of the known toxic effects of heroin. PMID: 23953641
    40. Results identified NOS1 as a novel nitric oxide target in human skeletal muscle controlled by activity driven auto-nitrosylation mechanisms. PMID: 24251120
    41. rADI not only reduced NO production but also caused cellular toxicity in nNOS-activated SH-SY5Y cells, suggesting a dual role for rADI in NOS-mediated neurotoxicity. PMID: 25126568
    42. DNA methylation of NOS1 plays role in atherogenesis through regulation of NO production. PMID: 24622112
    43. This review will focus on the role of Nitric Oxide Synthases (NOSes) the role they play in the pathogenesis of subarachnoid hemorrhage. PMID: 25366612
    44. The NOS1 rs41279104 CT genotype was associated with good responders in postoperative ED patients. PMID: 24897285
    45. Studied which NOS gene variants are most strongly associated with coronary heart disease (CHD) and hypertension and confirmed NOS1 as the most important NOS risk gene for CHD. PMID: 24713495
    46. Population genetic variability in Sardinia (Italy) shows a positive and highly significant correlation between mortality determined by malaria infection and alleles (TGGA)7 of NOS2, (AAAAG)2 and (ATTT)10 of adNOS1, and (AAACA)11 of adNOS3 genes. PMID: 24573959
    47. The resukts of this study demonistrated that the risk allele significantly decreases expression of NOS1 in the prefrontal cortex in patient with schizophrenia. PMID: 24220657
    48. Genetic or pharmacologic inhibition of NOS1 reduced the growth of CXCL14-expressing fibroblasts. PMID: 24710408
    49. The results showed that rs7308402 gene polymorphism of nNOS is related to ischemic stroke in Han Chinese of North China. PMID: 24082858
    50. A report on the reduction of NOS1 expression in the anterior cingulate cortex in depressive patients. PMID: 22989585

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  • 亚细胞定位：
    Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine.
  • 蛋白家族：
    NOS family
  • 组织特异性：
    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected
  • 数据库链接：

    HGNC: 7872

    OMIM: 163731

    KEGG: hsa:4842

    STRING: 9606.ENSP00000337459

    UniGene: Hs.654410