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  4. Human Neurogranin(NRGN) ELISA kit

Human Neurogranin(NRGN) ELISA kit

  • 中文名称：
    人神经颗粒素(NRGN)酶联免疫试剂盒
  • 货号：
    CSB-EL016081HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO’s Neurogranin(NRGN) ELISA kit is specifically designed and validated for the quantitative detection of NRGN in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for neuroscience research use in humans. The kit has been validated with excellent sensitivity, high specificity, precision less than 10%, good linearity, high recovery, and tiny inter-batch differences. Refer to the product instructions for more details.

    This assay employs the quantitative sandwich enzyme immunoassay technique, in which NRGN in the samples or standards are sandwiched between pre-coated NRGN antibody and Biotin-conjugated NRGN antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of NRGN bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of NRGN in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

    NRGN is a small neuronal protein present mainly in the cell body and dendrites. It primarily seems to regulate calmodulin (CaM) signal transduction pathways of CaM-dependent enzymes to enhance synaptic plasticity in long-term potentiation (LTP). Keeping NRGN concentrations in a normal range in neurons is essential for proper cognition, while a reduction of NRGN upon aging and degenerative diseases may lead to negative cognitive symptoms. Increased levels of NRGN has been detected in the cerebrospinal fluid (CSF) of patients with Alzheimer's disease (AD).

  • 别名：
    Protein kinase C substrate RC3 ELISA Kit; Calmodulin binding protein ELISA Kit; Hng ELISA Kit; NEUG(55-78) ELISA Kit; NEUG_HUMAN ELISA Kit; Neurogranin (protein kinase C substrate) ELISA Kit; Ng ELISA Kit; NRGN ELISA Kit; Protein kinase C substrate ELISA Kit; RC3 ELISA Kit
  • 缩写：
    NRGN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
  • 检测范围：
  • 灵敏度：
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
  • 线性度：
  • 回收率：
  • 标准曲线：
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NRGN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled NRGN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • 基因功能参考文献：
    1. The dynamics of calmodulin interactions with neurogranin and Ca(2+) /CAMKII alpha proteins has been reported. PMID: 28449373
    2. Plasma neurogranin did not correlate with stroke severity. PMID: 28854881
    3. Study observed an association of rs12807809 with schizophrenia in a South Indian population. This study contributes toward the establishment of neurogranin as a susceptibility gene for schizophrenia South Indian Population. PMID: 28389239
    4. associations of neuromodulin and neurogranin to Alzheimer's disease PMID: 27604409
    5. The results of this results showed that increased CSF neurogranin levels in Alzheimer Disease. PMID: 27018940
    6. Polymorphisms in NRGN are associated with schizophrenia, major depressive disorder and bipolar disorder in the Han Chinese population. PMID: 26828755
    7. Neurogranin and YKL-40 are promising AD biomarkers, independent of and complementary to the established core Alzheimer's disease (AD) biomarkers, reflecting additional pathological changes in the course of AD PMID: 26698298
    8. These results confirm an increase in CSF Ng concentration in patients with AD as previously reported and show that this is specific to AD and not seen in a range of other neurodegenerative diseases. PMID: 26826204
    9. This report provides evidence to support larger and controlled traumatic brain injury clinical studies for NRGN validation and prediction of outcomes. PMID: 26025774
    10. Within-person levels of NGRN increased in cognitively normal participants but not in patients with later stage mild cognitive impairment or Alzhiemer's disease; NGRN may reflect presymptomatic synaptic dysfunction or loss. PMID: 26366630
    11. Cerebrospinal fluid neurogranin was increased in patients with Alzheimer's dementia, progressive mild cognitive impairment (MCI) and stable MCI compared with controls, and in Alzheimer's dementia and progressive MCI compared with stable MCI. PMID: 26373605
    12. neurogranin binds to alpha-synuclein in the human cortex, and this interaction decreases in Parkinson's disease along with the phosphorylation of neurogranin, a molecular process thought to be involved in learning and memory PMID: 25446004
    13. These findings help to clarify underlying NRGN mediated pathophysiological mechanisms involving cortical-subcortical brain networks in schizophrenia. PMID: 24386483
    14. Data indicate that neurogranin makes contacts with both the N- and C-domains of calmodulin that functionally leads to altered calcium binding kinetics. PMID: 24713697
    15. NRGN risk variants contribute to subtle changes in neural functioning and anatomy. PMID: 24098564
    16. Our results support an association between the NRGN gene and schizophrenia and a hypothesis that the NRGN gene may mediate the risk associated with schizophrenia via intellectual dysfunction. PMID: 23903071
    17. The influence of NRGN genotype on the neural correlates of memory encoding and retrieval is manifest in the cingulate cortex and is involved in hippocampal formation. PMID: 21799211
    18. single nucleotide polymorphism located upstream of the neurogranin (NRGN) gene has been identified as a risk variant for schizophrenia. PMID: 22856365
    19. This study demonistrated that multiple rare mutations in schizophrenia, and provides genetic clues that indicate the involvement of NRGN in this disorder. PMID: 22306195
    20. This study provides further evidence of the association of the NRGN gene with schizophrenia. PMID: 22461181
    21. The genome-wide associated genetic risk variant in the NRGN gene may be related to a small gray matter volume in the anterior cingulate cortex in the left hemisphere in patients with schizophrenia. PMID: 22253779
    22. Hippocampal activation diminished during the acquisition of contextual fear in healthy carriers of the genome-wide-supported risk variant for schizophrenia, rs12807809 in neurogranin. PMID: 21647148
    23. our study provides strong evidence that common exonic variation does not account for the genome-wide signi fi cant association between schizophrenia and variation at NRGN PMID: 21538840
    24. Elevated cerebrospinal fluid neurogranin levels together with Alzheimer disease biomarkers may reflect synaptic degeneration. PMID: 20875798
    25. No association between the schizophrenia associated NRGN variant rs12807809 and cognition could be detected in these samples. PMID: 21112188
    26. IL-2 deprivation raises the level of RC3 and other apoptotic factors, which induce apoptosis by increasing the intracellular Ca(2+) concentration PMID: 12808095
    27. Nevertheless, by gel shift assays, Sp1 and Sp3 were not found to be responsible for the protein-DNA complexes formed by the GC-rich sequence. PMID: 16677608
    28. Genotype distribution showed association of the rs7113041 SNP with schizophrenia in males of Portuguese origin, which was confirmed by the analysis of the proband-parent triads. PMID: 17140601
    29. Data suggest that BSX is essential for global cognitive function and that haplo-insufficiency may cause severe mental retardation, and that deletion of Neurogranin contributes to the auditory attention deficit observed in most 11q- patients. PMID: 18855024

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  • 蛋白家族：
    Neurogranin family
  • 组织特异性：
    In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • 数据库链接：

    HGNC: 8000

    OMIM: 602350

    KEGG: hsa:4900

    STRING: 9606.ENSP00000284292

    UniGene: Hs.524116