Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human NGAL/MMP-9 complex ELISA kit

Human NGAL/MMP-9 complex ELISA kit

  • 中文名称：
    人NGAL/MMP-9复合物酶联免疫试剂盒
  • 货号：
    CSB-EQ01026975HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human NGAL/MMP-9 complex ELISA kit is suitable for quantitatively determining human NGAL/MMP-9 complex in multiple samples, including serum, plasma, saliva, cell culture supernates, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human NGAL/MMP-9 complex levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

    NGAL protects MMP-9 from proteolytic degradation and enhances its stability and enzymatic activities by binding and forming the NGAL/MMP-9 complex. The NGAL/MMP-9 complex is involved in the development and progression of cancer. NGAL/MMP-9 complex expression and activities have been detected in tissue and urine samples from patients with vascular anomalies and cancers. NGAL, MMP-9, and their complex NGAL/MMP-9 have been proposed as soluble biomarkers for numerous malignancies.

  • 缩写：
    NGAL/MMP-9 complex
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, saliva, cell culture supernates, tissue homogenates
  • 检测范围：
    0.313 ng/mL-20 ng/mL
  • 灵敏度：
    0.029 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10 Average % 91  
    Range % 87-98  
    1:20 Average % 95  
    Range % 90-102  
    1:40 Average % 93  
    Range % 87-99  
    1:80 Average % 94  
    Range % 86-105  
  • 回收率：
    The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-103  
    EDTA plasma (n=4) 94 87-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 1.955 2.004 1.980 1.857  
    10 1.637 1.629 1.633 1.510  
    5 1.204 1.217 1.211 1.088  
    2.5 0.894 0.907 0.901 0.778  
    1.25 0.573 0.582 0.578 0.455  
    0.625 0.416 0.424 0.420 0.297  
    0.313 0.281 0.270 0.276 0.153  
    0 0.124 0.121 0.123    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价