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Human Mucin-15(MUC15) ELISA kit

  • 中文名称：
    人粘蛋白-15(MUC15)酶联免疫试剂盒
  • 货号：
    CSB-EL015218HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MUC15 ELISA Kit was designed for the quantitative measurement of Human MUC15 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 别名：
    Component II ELISA Kit; Glycoprotein 4 ELISA Kit; Glycoprotein C ELISA Kit; MUC 15 ELISA Kit; MUC-15 ELISA Kit; Muc15 ELISA Kit; MUC15_HUMAN ELISA Kit; Mucin 15 ELISA Kit; Mucin 15 cell surface associated ELISA Kit; Mucin-15 ELISA Kit; PAS3 ELISA Kit; PASIII ELISA Kit
  • 缩写：
    MUC15
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MUC15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 89-103
    1:2 Average % 95
    Range % 90-99
    1:4 Average % 97
    Range % 87-102
    1:8 Average % 103
    Range % 97-108
  • 回收率：
    The recovery of human MUC15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 86 81-90
    EDTA plasma (n=4) 92 88-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 1.972 2.027 2.000 1.863
    4000 1.456 1.436 1.446 1.309
    2000 0.902 0.925 0.914 0.777
    1000 0.576 0.583 0.580 0.443
    500 0.430 0.421 0.426 0.289
    250 0.317 0.309 0.313 0.176
    125 0.250 0.242 0.246 0.109
    0 0.140 0.134 0.137 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May play a role in the cell adhesion to the extracellular matrix.
  • 基因功能参考文献：
    1. Frameshift mutations of MUC15 gene is associated with gastric and colorectal cancers. PMID: 25573589
    2. Multivariate analysis suggested that MUC15 overexpression was an independent factor for prognosis (hazard risk: 3.216; P=0.009). It was concluded that MUC15 is overexpressed in glioma tissues. PMID: 24710941
    3. MUC15 inhibits dimerization of EGFR and PI3K-AKT signaling and is associated with aggressive hepatocellular carcinomas in patients. PMID: 23933603
    4. MUC15 is a strong candidate for eczema. PMID: 22657408
    5. MUC4 and MUC15 were overexpressed in papillary thyroid carcinoma, and high MUC15 expression was associated with high malignant potential. PMID: 21615302
    6. MUC15 was expressed most highly in human placenta. MUC15 mRNA & protein increased with gestational age (P < 0.05, 1st versus 3rd trimester). Differential expression of MUC15 in human placentas may play critical role in regulation of trophoblast invasion. PMID: 17720698
    7. Overexpression of MUC15 activates extracellular signal-regulated kinase 1/2 and promotes the oncogenic potential of colon cancer cells. PMID: 19520792

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
  • 组织特异性：
    Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, bone marrow, lymph node and lung.
  • 数据库链接：

    HGNC: 14956

    OMIM: 608566

    KEGG: hsa:143662

    STRING: 9606.ENSP00000416753

    UniGene: Hs.407152