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  4. Human Mast Cell Carboxypeptidase ELISA Kit

Human Mast Cell Carboxypeptidase ELISA Kit

  • 中文名称：
    人肥大细胞类羧肽酶酶联免疫试剂盒
  • 货号：
    CSB-E13756h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CPA3 ELISA Kit was designed for the quantitative measurement of Human CPA3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 别名：
    carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %100
    Range %94-103
    1:4Average %97
    Range %94-100
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9694-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.477 2.453 2.465 2.366
    101.993 1.924 1.959 1.860
    51.280 1.271 1.276 1.177
    2.50.685 0.678 0.682 0.583
    1.250.433 0.424 0.429 0.330
    0.6250.286 0.269 0.278 0.179
    0.3120.190 0.179 0.185 0.086
    00.101 0.097 0.099 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. Carboxypeptidase A3 was differentially expressed between Fibromyalgia patients and healthy controls. PMID: 27157394
    2. Urine CPA3 was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID: 24726923
    3. The cDNA encoding human colon mast cell carboxypeptidase can be successfully cloned and expressed in E. coli and P. pastoris. PMID: 14760754
    4. Role in regulating innate immunity responses and regulating secretory granule homeostasis PMID: 19643669
  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules.
  • 蛋白家族：
    Peptidase M14 family
  • 数据库链接：

    HGNC: 2298

    OMIM: 114851

    KEGG: hsa:1359

    STRING: 9606.ENSP00000296046

    UniGene: Hs.646