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  4. Human Malate Dehydrogenase 1 (MDH1) ELISA Kit

Human Malate Dehydrogenase 1 (MDH1) ELISA Kit

  • 中文名称：
    人苹果酸脱氢酶1(MDH1)酶联免疫试剂盒
  • 货号：
    CSB-E13662h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MDH1 ELISA Kit was designed for the quantitative measurement of Human MDH1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.35 mU/mL.

  • 别名：
    cytoplasmic ELISA Kit; Cytosolic malate dehydrogenase ELISA Kit; Diiodophenylpyruvate reductase ELISA Kit; Malate dehydrogenase 1, NAD (soluble) ELISA Kit; Malate dehydrogenase ELISA Kit; Malate dehydrogenase cytoplasmic ELISA Kit; MDH s ELISA Kit; mdh1 ELISA Kit; MDHA ELISA Kit; MDHC_HUMAN ELISA Kit; MDHs ELISA Kit; MGC:1375 ELISA Kit; MOR2 ELISA Kit; Soluble malate dehydrogenase ELISA Kit
  • 缩写：
    MDH1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    9.38 mU/mL-600 mU/mL
  • 灵敏度：
    2.35 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MDH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %91-99
    1:2Average %102
    Range %97-107
    1:4Average %110
    Range %107-113
    1:8Average %86
    Range %83-89
  • 回收率：
    The recovery of human MDH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9188-94
    EDTA plasma (n=4)9491-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    6002.157 2.198 2.178 2.053
    3001.766 1.738 1.752 1.627
    1501.255 1.269 1.262 1.137
    750.764 0.798 0.781 0.656
    37.50.512 0.531 0.522 0.397
    18.750.305 0.324 0.315 0.190
    9.380.198 0.188 0.193 0.068
    00.126 0.124 0.125
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
  • 基因功能参考文献：
    1. expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in non-small cell lung carcinoma cell lines. PMID: 29574159
    2. Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific. PMID: 27881739
    3. Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD. PMID: 28263970
    4. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
    5. adipogenic differentiation may be regulated by the acetylation of MDH1 PMID: 22693256
    6. The MDH1 gene is not the cause of RP28-linked autosomal recessive retinitis pigmentosa. PMID: 20011630
    7. expression of MDH1 is maintained in the adult heart but is not present in levels as high as in the fetus PMID: 15565635
    8. Malate Dehydrogenase directly regulates the Tumor Suppressor Protein p53-dependent apoptosis upon glucose deprivation and involved in maintaining cellular metabolic state and further determining cell death. PMID: 19229245

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    LDH/MDH superfamily, MDH type 2 family
  • 数据库链接：

    HGNC: 6970

    OMIM: 154200

    KEGG: hsa:4190

    STRING: 9606.ENSP00000438144

    UniGene: Hs.526521