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  4. Human Lysosomal protective protein(CTSA) ELISA kit

Human Lysosomal protective protein(CTSA) ELISA kit

  • 中文名称：
    人溶酶体保护蛋白(CTSA)酶联免疫试剂盒
  • 货号：
    CSB-EL006184HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CTSA ELISA Kit was designed for the quantitative measurement of Human CTSA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    beta galactosidase 2 ELISA Kit; BETA GALACTOSIDASE PROTECTIVE PROTEIN ELISA Kit; beta-galactosidase 2 ELISA Kit; beta-galactosidase protective protein ELISA Kit; betagalactosidase 2 ELISA Kit; Carboxypeptidase C ELISA Kit; Carboxypeptidase L ELISA Kit; carboxypeptidase Y-like kininase ELISA Kit; Cathepsin A ELISA Kit; Ctsa ELISA Kit; deamidase ELISA Kit; EC 3.4.16.5 ELISA Kit; Glactosialidosis ELISA Kit; GLB2 ELISA Kit; Goldberg Syndrome ELISA Kit; GSL ELISA Kit; lysosomal carboxypeptidase A ELISA Kit; Lysosomal protective protein 20 kDa chain ELISA Kit; Lysosomal protective protein ELISA Kit; Lysosomal protective protein deficiency ELISA Kit; NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION; NGBE ELISA Kit; Neuraminidase deficiency with beta-galactosidase deficiency ELISA Kit; NGBE ELISA Kit; OTTHUMP00000031778 ELISA Kit; OTTHUMP00000031781 ELISA Kit; PPCA ELISA Kit; PPCA deficiency ELISA Kit; PPGB ELISA Kit; PPGB_HUMAN ELISA Kit; Protective protein cathepsin A ELISA Kit; Protective protein for beta galactosidase ELISA Kit; Protective protein for beta-galactosidase ELISA Kit; Protective protein/cathepsin A deficiency ELISA Kit; urinary kininase ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CTSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %89
    Range %85-93
    1:4Average %106
    Range %102-109
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of human CTSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-96
    EDTA plasma (n=4)9692-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.745 2.849 2.797 2.693
    5002.257 2.412 2.335 2.231
    2501.616 1.717 1.667 1.563
    1250.943 0.965 0.954 0.850
    62.50.547 0.524 0.536 0.432
    31.20.354 0.363 0.359 0.255
    15.60.245 0.254 0.250 0.146
    00.103 0.105 0.104
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
  • 基因功能参考文献：
    1. The usefulness of modified U1 snRNA for rescue from exon 7 skipping caused by the IVS7 +3a>g mutation of the CTSA gene. PMID: 30010039
    2. The gene signature of OPA1, CTSA, NDUFA1, STK10 and PRDX1 was able to identify patients post-implant with a sensitivity of 91% and a specificity of 86% in discrimination between post-implant group and healthy controls. PMID: 27177495
    3. Galactosialidosis is a rare lysosomal storage disease caused by a combined deficiency of GM1 beta-galactosidase (beta-gal) and neuraminidase secondary to a defect of a lysosomal enzyme protective protein/cathepsin A (PPCA) and mutation in CTSA gene. PMID: 26259553
    4. Case Report: galactosialidosis with novel mutations of CTSA gene diagnosed using placental pathology. PMID: 25075748
    5. We identified compound heterozygous mutations in the CTSA gene, responsible for causing galactosialidosis PMID: 24769197
    6. correct nomenclature of mutations for this gene is discussed; clinical and mutational analyses of 4 cases with rare infantile form of galactosialidosis; identified 3 novel nucleotide changes, 2 resulting in missense mutations and the third, resulting in the p.Gln406* stop codon; complexity of the clinical phenotypes in GS reflects dual functions of PPCA/CTSA PMID: 23915561
    7. Catalytic function, tissue distribution and substrates of cathepsin A are discussed as well as inhibition of cathepsin A as an emerging strategy for the treatment of heart failure. PMID: 23495688
    8. The Cathepsin C releases the glycosidases from complexes formed with cathepsin A, and reinstates their activity. PMID: 22532132
    9. Our data suggest that CatA is involved in the C-terminal fine-tuning of antigenic T cell epitopes in human APC. PMID: 19954752
    10. mutations in early infantile galactosialidosis in two Dutch patients PMID: 12649068
    11. Increased activity of beta-galactosidase in the peritoneal fluid is associated with gynecologic cancers and pelvic inflammatory disease PMID: 15785934
    12. effects of GLB1, PPCA and NEU1 gene mutations on elastogenesis in skin fibroblasts PMID: 16538002
    13. Results describe the hydrodynamic properties of PPCA, NEU1, and a complex of the two proteins and identified multiple binding sites on both proteins. PMID: 19666471

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  • 相关疾病：
    Galactosialidosis (GSL)
  • 亚细胞定位：
    Lysosome.
  • 蛋白家族：
    Peptidase S10 family
  • 数据库链接：

    HGNC: 9251

    OMIM: 256540

    KEGG: hsa:5476

    STRING: 9606.ENSP00000361562

    UniGene: Hs.609336