Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Lipocalin-1(LCN1) ELISA kit

Human Lipocalin-1(LCN1) ELISA kit

  • 中文名称：
    人载脂运载蛋白-1(LCN1)酶联免疫试剂盒
  • 货号：
    CSB-EL012810HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LCN1 ELISA Kit was designed for the quantitative measurement of Human LCN1 protein in serum, plasma, tissue homogenates, saliva. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    LCN1 ELISA Kit; LCN1_HUMAN ELISA Kit; Lipocalin 1 (protein migrating faster than albumin, tear prealbumin) ELISA Kit; Lipocalin 1 like 2 ELISA Kit; Lipocalin-1 ELISA Kit; PMFA ELISA Kit; Tear lipocalin ELISA Kit; Tear prealbumin ELISA Kit; Tlc ELISA Kit; TP ELISA Kit; VEG protein ELISA Kit; VEGP ELISA Kit; Von Ebner gland protein ELISA Kit
  • 缩写：
    LCN1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, saliva
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LCN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of human LCN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9290-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.714 2.768 2.741 2.654
    501.812 1.801 1.807 1.720
    251.005 1.058 1.032 0.945
    12.50.538 0.568 0.553 0.466
    6.250.301 0.325 0.313 0.226
    3.120.245 0.238 0.242 0.155
    1.560.142 0.156 0.149 0.062
    00.088 0.086 0.087
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
  • 基因功能参考文献：
    1. At the relatively small concentrations in tears, all ceramides were complexed to tear lipocalin. PMID: 29331331
    2. Human neutrophil lipocalin (HNL) concentrations in serum or whole blood activated by formyl-methionine-leucine-phenylalanine (fMLP) were shown to distinguish acute infections of bacterial or viral cause with high accuracy. PMID: 26135974
    3. the possible relationship between ocular symptomatology, tear volume and tear break-up time (TBUT) and lipocalin, lactoferrin and lysozyme concentrations in the tear film were explored in a group of symptomatic dry-eyed postmenopausal (PM) women. PMID: 26366224
    4. Tear lysozyme, lactoferrin and lipocalin concentrations were determined via electrophoresis and the results for patients with or without eyelid tumors were compared. PMID: 25914748
    5. the crosstalk of dendritic cells with lipocalins alone has the potential to direct the type of immune response to these particular antigens. PMID: 26218644
    6. Serum concentrations of LCN1 and LCN2 were both elevated in patients with COPD. PMID: 24920892
    7. Tear lipocalin (TLC) is a major protein in tears and has a large ligand-binding cavity that allows the lipocalin to bind an extensive and diverse set of lipophilic molecules. PMID: 21791187
    8. Study has identified the first protein, lipocalin-1, in the secretome of human blastocysts that is associated with chromosome aneuploidy. PMID: 21324447
    9. The conserved disulfide bond of human tear lipocalin modulates conformation and lipid binding in a ligand selective manner.( PMID: 21466861
    10. Tear lipocalin with the bound artificial ligand 1,4-butanediol has been crystallized in space group P2(1) with four protein molecules in the asymmetric unit and its X-ray structure has been solved at 2.6 A resolution. PMID: 19770509
    11. The N-terminal part of recombinant human tear lipocalin/von Ebner's gland protein confers cysteine proteinase inhibition depending on the presence of the entire cystatin-like sequence motifs. PMID: 11727836
    12. Lcn-1 is specifically produced by corticotrophs PMID: 11850445
    13. LIMR mediating internalization of lipocalin-1 (Lcn-1) in NT2 cells, leading to its degradation. PMID: 12591932
    14. structure, function, and mechanism of action [review] PMID: 12613960
    15. Tear lipocalin is probable protein carrier for vitamin E tears and is candidate to specifically deliver vitamin E as antioxidant to corneal epithelium. PMID: 12613961
    16. Tear surface tension depends partly on presence of tear lipocalin/lipid complex. Tear proteins and lipid-free tear lipocalin lower surface tension. PMID: 12613963
    17. pH-driven ligand release involves ionization changes in several titratable residues associated with CD and EF loop apposition and occlusion of the calyx PMID: 15461462
    18. structural analysis sheds new light on the ligand binding activity of this functionally obscure but abundant human lipocalin PMID: 15489503
    19. Lipocalin is a novel autoantigen target in Sjogren's syndrome. PMID: 16249071
    20. Hydrodynamic and solution properties of lipocalin from human tears show that the protein size and topology are the major determinants of rotational correlation time and a reason for deviation from the Stokes-Einstein relationship. PMID: 17869594
    21. Selective displacement of ANS molecules from the ANS-apoTL complex by stearic acid discriminates the internal and external binding sites. PMID: 17945179
    22. Tear lipocalin is the principal endonuclease in human tear fluid. PMID: 18334931
    23. Results suggest that the excited protein states in tear lipocalin are not unique but consist of many substates. PMID: 19586017

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Mainly expressed in lachrymal and salivary glands. Also expressed in the prostate.
  • 数据库链接：

    HGNC: 6525

    OMIM: 151675

    KEGG: hsa:3933

    STRING: 9606.ENSP00000263598

    UniGene: Hs.530311